FastQCFastQC Report
Mon 28 Nov 2022
S1_Sudafrica.EVE_segment_M.15-35nt.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS1_Sudafrica.EVE_segment_M.15-35nt.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2721
Sequences flagged as poor quality0
Sequence length15-30
%GC1

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA1876.87247335538405No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA1666.100698272693863No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA1495.475927967658949No Hit
AAAAAAAAAAAAAAAAAAAAAAA1475.402425578831313No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA1425.218669606762219No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA1375.034913634693128No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1344.9246600514516725No Hit
AAAAAAAAAAAAAAAAAAAAA1294.740904079382579No Hit
AAAAAAAAAAAAAAAAAAAAAAAA1274.667401690554943No Hit
AAAAAAAAAAAAAAAAAAA1224.4836457184858505No Hit
AAAAAAAAAAAAAAAAAAAAAA1214.446894524072032No Hit
AAAAAAAAAAAAAAAAAAAA1114.0793825799338475No Hit
AAAAAAAAAAAAAAAAA1053.8588754134509373No Hit
AAAAAAAAAAAAAAAA1043.8221242190371187No Hit
AAAAAAAAAAAAAAAAAA1043.8221242190371187No Hit
AAAAAAAAAAAAAAA963.528114663726571No Hit
GGAAAAAAAAAAAAAAAAAAAAAA230.8452774715178243No Hit
GGAAAAAAAAAAAAAAAAAAAAAAA180.6615214994487321No Hit
GGAAAAAAAAAAAAAAAAAAAA110.40426313855200297No Hit
GTTAAAAAAAAAAAAAAAAAAAAAAA110.40426313855200297No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA100.3675119441381845No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA90.33076074972436603No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAA90.33076074972436603No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.2940095553105476No Hit
TAAAAAAAAAAAAAAA70.25725836089672915No Hit
GTTAAAAAAAAAAAAAAAAAA70.25725836089672915No Hit
GGGAAAAAAAAAAAAAAAAAAAAA70.25725836089672915No Hit
GTTAAAAAAAAAAAAAAAAA70.25725836089672915No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAA70.25725836089672915No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAA60.2205071664829107No Hit
CAAAAAAAAAAAAAAAAAAAAAAA60.2205071664829107No Hit
GTTAAAAAAAAAAAAAAAAAAAAAAAA60.2205071664829107No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAA60.2205071664829107No Hit
CAAAAAAAAAAAAAA60.2205071664829107No Hit
GGAAAAAAAAAAAAAAAA60.2205071664829107No Hit
GTTTAAAAAAAAAAAAAAAAAAAAAA50.18375597206909225No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAA50.18375597206909225No Hit
TAAAAAAAAAAAAAAAAAAAAAA50.18375597206909225No Hit
GTTGAAAAAAAAAAAAAAAAAAAAAA50.18375597206909225No Hit
CAAAAAAAAAAAAAAAAAAAA50.18375597206909225No Hit
GAAAAAAAAAAAAAAAAAAAAAAA50.18375597206909225No Hit
GTTTAAAAAAAAAAAAAAAAAAAAAAA50.18375597206909225No Hit
GTTAAAAAAAAAAAA40.1470047776552738No Hit
GAAAAAAAAAAAAAAAA40.1470047776552738No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1470047776552738No Hit
GGAAAAAAAAAAAAAAAAAAAAA40.1470047776552738No Hit
TAAAAAAAAAAAAAAAAAAAA40.1470047776552738No Hit
GGAAAAAAAAAAAAAAAAAA40.1470047776552738No Hit
GAAAAAAAAAAAAAAAAA40.1470047776552738No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1470047776552738No Hit
GAAAAAAAAAAAAAAAAAAAA40.1470047776552738No Hit
AAACAAAAAAAAAAAAAAAAAAAAAA40.1470047776552738No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAA40.1470047776552738No Hit
GAAAAAAAAAAAAAAAAAAAAA30.11025358324145534No Hit
AAAAAAAAAAAAACAAAAAAAAAAAA30.11025358324145534No Hit
CAAAAAAAAAAAAAAAAAA30.11025358324145534No Hit
GGGAAAAAAAAAAAAAAAAAAAAAA30.11025358324145534No Hit
AAAAAAAAAAAAAAAAAAAAAAT30.11025358324145534No Hit
TGTAAAAAAAAAAAAAAAAAAAAAAA30.11025358324145534No Hit
GAAAAAAAAAAAAAAAAAA30.11025358324145534No Hit
GGAAAAAAAAAAAAAA30.11025358324145534No Hit
GTTAAAAAAAAAAAAAAAA30.11025358324145534No Hit
TAAAAAAAAAAAAAAAAA30.11025358324145534No Hit
GTAAAAAAAAAAAAA30.11025358324145534No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA30.11025358324145534No Hit
TGTAAAAAAAAAAAAAAAAAAAAAAAAAA30.11025358324145534No Hit
AGTAAAAAAAAAAAAAAAAAAAAA30.11025358324145534No Hit
GTTTAAAAAAAAAAAAAAAA30.11025358324145534No Hit
GAAAAAAAAAAAAAA30.11025358324145534No Hit
GTTAAAAAAAAAAAAAAAAAAA30.11025358324145534No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAA30.11025358324145534No Hit
CAAAAAAAAAAAAAAA30.11025358324145534No Hit
CAAAAAAAAAAAAAAAAAAAAAAAA30.11025358324145534No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAA30.11025358324145534No Hit
GAAAAAAAAAAAAAAAAAAAAAA30.11025358324145534No Hit
GTAAAAAAAAAAAAAAAAA30.11025358324145534No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)