FastQCFastQC Report
Mon 28 Nov 2022
S1_Sudafrica.Phlebovirus_segment_L.15-35nt.new.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS1_Sudafrica.Phlebovirus_segment_L.15-35nt.new.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1289
Sequences flagged as poor quality0
Sequence length20-35
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACATTGACTGGCCAAAGAATAATAAGACTCC211.6291698991466252No Hit
AAACATTGACTGGCCAAAGAATAATAAGACTCCA181.3964313421256789No Hit
TCTAAACATTGACTGGCCAAAGAATAATAAGACTC151.1636927851047323No Hit
TTGTGAAGGAGGGTATAGCCAGTCGAGGACTTGC141.0861132660977502No Hit
GTGTATTTACAAGGAACAGTCAGACGAAATTCAAC131.008533747090768No Hit
TGTCGAATCACCTTCATCCATCATGCATTCTTCC120.9309542280837859No Hit
CAGGGTCCAGAATCTTCTTTAACAATTTACTTGAG110.8533747090768037No Hit
CCTGTAACCTCCCGATATCACTAACATTCTTGAAA110.8533747090768037No Hit
AACATTGACTGGCCAAAGAATAATAAGACTCCA110.8533747090768037No Hit
AGAGAGATGATCTTGGAAGGGACACTGAGCT110.8533747090768037No Hit
TCTTTCACCTTGGCTGTCTGCAAATTTTTCCT110.8533747090768037No Hit
TTCTTCAACAGTTTTTTGCAGTAGGCTCGGCACA100.7757951900698216No Hit
TAAACATTGACTGGCCAAAGAATAATAAGACTCCA100.7757951900698216No Hit
CTGGATGAGTCATGATTTCTGATTGAAATTGC100.7757951900698216No Hit
TTTGCAGCTCATTCTAACTCTTTCACCTTGGC100.7757951900698216No Hit
ATTGGCATCACTCTTCTATTATCTACGCATCCTC100.7757951900698216No Hit
TTTCCTGTAACCTCCCGATATCACTAAC100.7757951900698216No Hit
CCAGAGTCTGGTTTTTCTGTACTCATTCATTGT100.7757951900698216No Hit
AGACAAGTAATTTTCAACGTGATCAGGCAGGTCGA90.6982156710628394No Hit
CACTTAGAACCATTCTCCTAGTTTTGTATTCCACT90.6982156710628394No Hit
TCCAAGGACTGATAGAAGCCCGAGCTTGCTTTC90.6982156710628394No Hit
TTGTTGGGAGGTTTTCTGCTGGTCTTGGA90.6982156710628394No Hit
GCTAATCTGTAAGGCACCTGATTCGGAACGCTGG90.6982156710628394No Hit
TGAACACCGTAGAGACAGTGGAGCCCAAAAGTCT90.6982156710628394No Hit
TCCCTGGCAGCTGACTCTTTCCTGTAACCTCCCG90.6982156710628394No Hit
TTCCGGAAGCAGACCACACAACATGATGGCAAA80.6206361520558572No Hit
TGATCTTGGAAGGGACACTGAGCTAGAGTCAAAT80.6206361520558572No Hit
TGTATTCGTATAGCTTGGGTGACTCCATCCTTCTC80.6206361520558572No Hit
AATGCAGCTTTCACTACAGGAATGGGAAGGGGA80.6206361520558572No Hit
ACATTGACTGGCCAAAGAATAATAAGACTCCA80.6206361520558572No Hit
CTGGCAGCTGACTCTTTCCTGTAACCTCCCGA80.6206361520558572No Hit
TCTCATAGACTTGTTCATCCTCGAAGACCATCAC80.6206361520558572No Hit
TTCCGGAAGCAGACCACACAACATGATGGCAAAC80.6206361520558572No Hit
TTCCTGTAACCTCCCGATATCACTAACATTC80.6206361520558572No Hit
CGGGAGAACTAGTATCCAGATGAGGCCTAAAG80.6206361520558572No Hit
GTTTTTTGCAGTAGGCTCGGCACAGGTTGT70.5430566330488751No Hit
TGTCACTGATTGAAATTGTGAAGGAGGGTATAGC70.5430566330488751No Hit
CTTCATAAAACCAATCATGATTGTCCAGAACCTC70.5430566330488751No Hit
CGGAAGCAGACCACACAACATGATGGCAAAC70.5430566330488751No Hit
CCAGAACCCAAAAGTGAAATCATGTATGAATG70.5430566330488751No Hit
ATCTCATAGACTTGTTCATCCTCGAAGACCATCAC70.5430566330488751No Hit
TGCATACCTGTACCTGGCAACCAGCTCATCCA70.5430566330488751No Hit
CATTGACTGGCCAAAGAATAATAAGACTCC60.46547711404189296No Hit
CGTGCTCCTTCCTCTGATAGCTCTTATTGG60.46547711404189296No Hit
GCAAGATCATCATCTGAGACACCACAAT60.46547711404189296No Hit
ATTAGCTGTTTTACCACCCATACTTCTAGATTG60.46547711404189296No Hit
AGAGACAGTGGAGCCCAAAAGTCTGTAAAA60.46547711404189296No Hit
TGAGACTGCGCATGCATACCTGTACCTGGCAACC60.46547711404189296No Hit
TTTGCAGCTCATTCTAACTCTTTCACCAG60.46547711404189296No Hit
CCAGAGATTACTAGCCCACACGTCGGCAGG60.46547711404189296No Hit
AGTAATTTTCAACGTGATCAGGCAGGT60.46547711404189296No Hit
CACCCCTCTTCATCCATGGCTCTCCCCTAACT60.46547711404189296No Hit
TTCCTGTAACCTCCCGATATCACTAACATTCT60.46547711404189296No Hit
TACTTCAGTTTCTTTGCAATCCATTGGCCTTTCT60.46547711404189296No Hit
AGAGATGATCTTGGAAGGGACACTGAGCTAGAG60.46547711404189296No Hit
TCACTTAGAACCATTCTCCTAGTTTTGTATTCC60.46547711404189296No Hit
TGAGACTGCGCATGCATACCTGTACCTGGCAACCA60.46547711404189296No Hit
CCATTTTTGTCTCACTAGGAAAATTCCATGCACTC60.46547711404189296No Hit
TTTTGTCTCACTAGGAAAATTCCATGCACTCCAAA60.46547711404189296No Hit
TTCTAACTCTTTCACCTTGGC60.46547711404189296No Hit
ATTCTCAGTTTGTCCATCATGCCAGCCCACTCAA60.46547711404189296No Hit
TTCTAACTCTTTCACCTTGGCTGTCTGCAAATT60.46547711404189296No Hit
TTTTATGTCACTCTCTTTCGCCCATCTTTTTAGCA60.46547711404189296No Hit
AATCTGTAAGGCACCTGATTCGGAACGCTGGCTC60.46547711404189296No Hit
TCATTTAAAGGATTCCCGTAGATCAT60.46547711404189296No Hit
TTAAGCACTGTTTCACCGCCCACGACCTGAACACC50.3878975950349108No Hit
CCTGAACACCGTAGAGACAGTGGAGCCCAA50.3878975950349108No Hit
TTCCTGTAACCTCCCGATATCACTAAC50.3878975950349108No Hit
CAGCTGACTCTTTCCTGTAACCTCCCGATATCAC50.3878975950349108No Hit
ACTGGCCAAAGAATAATAAGACTCCAA50.3878975950349108No Hit
ATGAGAGAGCCGTCGATGAAG50.3878975950349108No Hit
CCTGTAACCTCCCGATATCACTAACATTCT50.3878975950349108No Hit
AGGAACAGTCAGACGAAATTCAACAGTCTACCAG50.3878975950349108No Hit
GTTTTGCTGAGAACGACAGGTACTCCTA50.3878975950349108No Hit
CTTGGAAGGGACACTGAGCTAGAGTCAAA50.3878975950349108No Hit
TTCAGTTTCTTTGCAATCCATTGGCCTTTCT50.3878975950349108No Hit
GTTCATAGATGTAAACCGCCGATTCACCA50.3878975950349108No Hit
CTTTCACCTTGGCTGTCTGCAAATTTTTCC50.3878975950349108No Hit
TGACTACTGGCTCGGGAGAAC50.3878975950349108No Hit
GCTTGTTTGAGACTGCGCATGCATACCTGTAC50.3878975950349108No Hit
CCTCATTTAAAGGATTCCCGTAGATCAT50.3878975950349108No Hit
CCCTAGCAGCTGACTCTTTCCTGTAACCTCCCGA50.3878975950349108No Hit
TAATTCTGTCGAATCACCTTCATCC50.3878975950349108No Hit
ATGGCTCTCCCCTAACTCCTCTGGTGGCTTCGT50.3878975950349108No Hit
CTTTCCTGTAACCTCCCGATATCACTAACA50.3878975950349108No Hit
GTACACTTTGGATGACTCTAGCTTCAGCAC50.3878975950349108No Hit
ATTAGCTGTTTTACCACCCATACTTCTAG50.3878975950349108No Hit
TTCATCAGTCGCATTGAAACTGGAAGGCAGGAGT50.3878975950349108No Hit
AAGGAGGGTATAGCCAGTCGAGGA50.3878975950349108No Hit
TCTTTCCTGTAACCTCCCGATATCACTA50.3878975950349108No Hit
TGACTGGCCAAAGAATAATAAGACTCCA50.3878975950349108No Hit
TTTCACCTTGGCTGTCTGCAAATTTTTCCTCA50.3878975950349108No Hit
AACAGTTTTTTGCAGTAGGCTCGGCACAGGTTG40.3103180760279286No Hit
AATTCTGTCGAATCACCTTCATCCA40.3103180760279286No Hit
AGCCCACACGTCGGCAGGAACGGGACTGGTCC40.3103180760279286No Hit
TGGATGACTCTAGCTTCAGCACTGAGGACGAA40.3103180760279286No Hit
TGAACACCGTAGAGACAGTGGAGCCCAAA40.3103180760279286No Hit
AAACATTGACTGGCCAAAGAATAATAAGACTCC40.3103180760279286No Hit
TACAAGGAACAGTCAGACGAAATTCAA40.3103180760279286No Hit
CTTGGCTGTCTGCAAATTTTTCCTCATAATC40.3103180760279286No Hit
CCGATCTGTGCCTTCACTCCAGAACCC40.3103180760279286No Hit
CCGTAGAGACAGTGGAGCCCAAAAGT40.3103180760279286No Hit
TCATTTAAAGGATTCCCGTAGATCATAGAG40.3103180760279286No Hit
TCTTTCCTGTAACCTCCCGATATCACTAA40.3103180760279286No Hit
ATCAATATGTGCGCTGGATCATCTGAAA40.3103180760279286No Hit
TTGAATCATTGTATGGAACATGGAGCTGGTGTAG40.3103180760279286No Hit
CAACAGTTTTTTGCAGTAGGCTCGGCACA40.3103180760279286No Hit
AAAACTTAATTAGCTGTTTTACCACCCATAC40.3103180760279286No Hit
CCAGTCACCCTCTAGATATCTATCAATCGAG40.3103180760279286No Hit
CTTGTCTCGATAACTCGTGATAGATTTGCCTAGC40.3103180760279286No Hit
TTAGCTCATCCACTCTGAACCCAGC40.3103180760279286No Hit
CATAGACTTGTTCATCCTCGAAGACCATCACATC40.3103180760279286No Hit
CAGTCGCATTGAAACTGGAAGGCAGGA40.3103180760279286No Hit
ACTCTTTCCTGTAACCTCCCGA40.3103180760279286No Hit
CATTGACTGGCCAAAGAATAATA40.3103180760279286No Hit
AAAACCTAATTCTGTCGAATCACCTTCATCCA40.3103180760279286No Hit
CTCCATAAAATCTGGAGTCCGGATCGTAC40.3103180760279286No Hit
TGTGCCTTCACTCCAGAACCC30.23273855702094648No Hit
TATGAGAGAGCCGTCGATGAAG30.23273855702094648No Hit
CCTGTAACCTCCCGATATCACT30.23273855702094648No Hit
AGCTCTGTTGCGACGTCTGCC30.23273855702094648No Hit
GACAAGTAATTTTCAACGTGATCAGGCAGGTC30.23273855702094648No Hit
CTCAGGGTCCAGAATCTTCTTTAACAA30.23273855702094648No Hit
TCTGTCCTGTCTCCGACTCCGCTAAGT30.23273855702094648No Hit
TAATTCTGTCGAATCACCTTCATCCATC30.23273855702094648No Hit
CACCGTAGAGACAGTGGAGCCCAAAAGT30.23273855702094648No Hit
ACAGTTTTTTGCAGTAGGCTCGGCACAG30.23273855702094648No Hit
TCCTGTAACCTCCCGATATCACTAACATTCT30.23273855702094648No Hit
AGGATTCCCGTAGATCATAGAGTCATTGTGTA30.23273855702094648No Hit
TTGGCATCACTCTTCTATTATCTACGCATCC30.23273855702094648No Hit
TTCTTTGCAATCCATTGGCCTTTCTTGCA30.23273855702094648No Hit
CCTGAACACCGTAGAGACAGTGGAGCCCAAAA30.23273855702094648No Hit
ATTTTTCCTCATAATCGTTCAGTTCTGCCAAC30.23273855702094648No Hit
TTTTGCAGTAGGCTCGGCACAGGTTGTT30.23273855702094648No Hit
TTCCTGTAACCTCCCGATATCACTAACA30.23273855702094648No Hit
ACAGGTTTAGTAGTGCTGAGCAGACTCGAGGC30.23273855702094648No Hit
TAGCAAAGTCATCACTTAGAACCATTCTCCTA30.23273855702094648No Hit
AGTTTCATCTCTGGATGAGTCATGATTTCTGATTG30.23273855702094648No Hit
CCTGTAACCTCCCGATATCAC30.23273855702094648No Hit
GAGATGATCTTGGAAGGGACACTGAGCT30.23273855702094648No Hit
CACAGGTTTAGTAGTGCTGAGCAGACTCGAGGC30.23273855702094648No Hit
TGTACCAACTTCAGCCTCTAA30.23273855702094648No Hit
CGTCTGCCATGAGTCTGCTCCACCACC30.23273855702094648No Hit
TCCTGTAACCTCCCGATATCACTAACAT30.23273855702094648No Hit
ATTGACTGGCCAAAGAATAATAAGACTCCA30.23273855702094648No Hit
AACACCGTAGAGACAGTGGAGCCCAAA30.23273855702094648No Hit
TTCCTGTAACCTCCCGATATC30.23273855702094648No Hit
TTGACCCAGAGAGTCTCGCAG30.23273855702094648No Hit
ACTCTTTCACCTTGGCTGTCT30.23273855702094648No Hit
GAATGACTGTCTTCTTCCCATTGGAT30.23273855702094648No Hit
CAACAGGCAGGCACCTCCCTCTAAACATTGACTGG30.23273855702094648No Hit
TTGACTGGCCAAAGAATAATAAGACTCCA30.23273855702094648No Hit
CGACCTGAACACCGTAGAGAC30.23273855702094648No Hit
ATATATTGACTGATACCTTTCCCATATTGA30.23273855702094648No Hit
CGTAGAGACAGTGGAGCCCAA30.23273855702094648No Hit
GGCAAGATCATCATCTGAGACACCACAATC30.23273855702094648No Hit
TAAACATTGACTGGCCAAAGAATAATAAG30.23273855702094648No Hit
AAAGGATTCCCGTAGATCATAGAGTC30.23273855702094648No Hit
GTGGTGTCATTATGGATAGAAAAAGAC30.23273855702094648No Hit
GTATCCCATTAATTCGTCGCCTTGTCTAAAGC30.23273855702094648No Hit
TGCAGTAGGCTCGGCACAGG30.23273855702094648No Hit
CGTAACCAGCACTTTGTACAAC30.23273855702094648No Hit
ATCCGGCAACTTTATTTTCTT20.1551590380139643No Hit
GTAAAACATCCAAGATTGAGAC20.1551590380139643No Hit
AACAGTCAGACGAAATTCAACAGT20.1551590380139643No Hit
CCTCAGGGTCCAGAATCTTCTTTAACAATTTACT20.1551590380139643No Hit
TTCATTAAGCACTGTTTCACCGCCC20.1551590380139643No Hit
ATCTGCACTGGTGACTGCTGATTTGCAAAAGT20.1551590380139643No Hit
CTAAACATTGACTGGCCAAAGAATAAT20.1551590380139643No Hit
CCGGAAGCAGACCACACAACATGATG20.1551590380139643No Hit
CACCCCTCTTCATCCATGGCTCTCCCCTAAC20.1551590380139643No Hit
AATTCTGTCGAATCACCTTCATCCATCATGCA20.1551590380139643No Hit
ATTCGCACGTAGGAGCCACCAGTCA20.1551590380139643No Hit
GAGAGAGCCGTCGATGAAGAA20.1551590380139643No Hit
ACATTGACTGGCCAAAGAATAATAAGACTCCAA20.1551590380139643No Hit
TATGTATTCTCCTTTGTCACTGATTGA20.1551590380139643No Hit
CTGGCAGGTTCATGGTTCTCAACAA20.1551590380139643No Hit
TTTTTGCAGTAGGCTCGGCACC20.1551590380139643No Hit
TCTCATAGACTTGTTCATCCTCGAAGACC20.1551590380139643No Hit
TTGAGACTGCGCATGCATACCTGTACCTGGCAACC20.1551590380139643No Hit
CAAGTCTAGTAACTCATCCGG20.1551590380139643No Hit
CTCTTTCCTGTAACCTCCCGA20.1551590380139643No Hit
TGCAGCTTTCACTACAGGAATGGGAAGGGGA20.1551590380139643No Hit
TCCCATATTGAATCTCTCACT20.1551590380139643No Hit
GTAGAGACAGTGGAGCCCAA20.1551590380139643No Hit
GTTGATTTGTATTCGTATAGCTTGGGTGACTCCA20.1551590380139643No Hit
CATTGACTGGCCAAAGAATAATAAGACTCCAA20.1551590380139643No Hit
TGCCTGAGACTGATGGATAGC20.1551590380139643No Hit
TATGGTGCATTTAACTGAGGGGTATATTC20.1551590380139643No Hit
CAAGTCTAGTAACTCATCCGGC20.1551590380139643No Hit
GCGCCCTAGCTTGATATGTCAGTATC20.1551590380139643No Hit
ATGTAAACCGCCGATTCACCA20.1551590380139643No Hit
TCTTTCCTGTAACCTCCCGATATCACTAACAT20.1551590380139643No Hit
CTGTAACCTCCCGATATCAC20.1551590380139643No Hit
ATTTACAAGGAACAGTCAGACGAAATTCAACAGT20.1551590380139643No Hit
ATAGACTTGTTCATCCTCGAAGACC20.1551590380139643No Hit
CTGTAACCTCCCGATATCACTAACATTCTTGAAA20.1551590380139643No Hit
TCTACGCATCCTCTTACCCATGTTTCATTCAATGT20.1551590380139643No Hit
CTCTAAACATTGACTGGCCAAAGAAT20.1551590380139643No Hit
CTCTAAACATTGACTGGCCAAAGAATAATAAG20.1551590380139643No Hit
TCCTTCGAGATGGTTACTGAA20.1551590380139643No Hit
ACTATGTCAAAGTCTGCAGCGCA20.1551590380139643No Hit
TGGGAGGACAGATGAGAATTCAACT20.1551590380139643No Hit
TATGAGAGAGCCGTCGATGA20.1551590380139643No Hit
CGCATGCATACCTGTACCTGGCAACCAGC20.1551590380139643No Hit
AAGCACTGTTTCACCGCCCACGAC20.1551590380139643No Hit
CGTCTGCCATGAGTCTGCTCCACC20.1551590380139643No Hit
TCTAACTCTTTCACCTTGGCTGTCTGC20.1551590380139643No Hit
AAACCTAATTCTGTCGAATCACC20.1551590380139643No Hit
TTTCATAGCTGCCTCGGAAAC20.1551590380139643No Hit
ACCACCCATACTTCTAGATTG20.1551590380139643No Hit
TTATGAGAGAGCCGTCGATGAAGAACT20.1551590380139643No Hit
CATTCTCAGTTTGTCCATCATGCCAGCCCA20.1551590380139643No Hit
TTTAGTAGTGCTGAGCAGACTCGAGG20.1551590380139643No Hit
GAAGGAGGGTATAGCCAGTCGAGGA20.1551590380139643No Hit
CTATGTCAAAGTCTGCAGCGCCA20.1551590380139643No Hit
CAAGTCTAGTAACTCATCCGGCAC20.1551590380139643No Hit
AACGCTGGGAGGACAGATGAGAATTCAACT20.1551590380139643No Hit
TTTCAAAACCTAATTCTGTCGAATCACCT20.1551590380139643No Hit
TTTTACCACCCATACTTCTAGATTGC20.1551590380139643No Hit
ACAGGTTTAGTAGTGCTGAGCAGACTCGAG20.1551590380139643No Hit
CGACGTCTGCCATGAGTCTGCTCCACCA20.1551590380139643No Hit
GTATAACCTTCTGTCACTGCTC20.1551590380139643No Hit
CATTTTTATAGCTAATCTGTAAGGCACC20.1551590380139643No Hit
AGTGCTGAGCAGACTCGAGGC20.1551590380139643No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)