FastQCFastQC Report
Mon 28 Nov 2022
S1_Sudafrica.Phlebovirus_segment_M.15-35nt.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS1_Sudafrica.Phlebovirus_segment_M.15-35nt.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206
Sequences flagged as poor quality0
Sequence length15-30
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTTTGAAGCACAGGTAGCATGATCCG125.825242718446602No Hit
CAACATGCTGATCAGCCCCTTAGATATCCA83.8834951456310676No Hit
TAGGTCGCGTTGTCGCTCTTGCACTCGAAT62.912621359223301No Hit
TGAAGCACAGGTAGCATGATCCGAGAG52.4271844660194173No Hit
GCGGCGCTGTGATGGACACCAAGCTAAC52.4271844660194173No Hit
TCTTAGCCGTGATGATCTCTCCTTTCAC52.4271844660194173No Hit
GTTCTGTAGGTTTCTGAGTGGCTTGG41.9417475728155338No Hit
CTGATTCAATGCCATCTCTTTCCACC41.9417475728155338No Hit
TTTGAAGCACAGGTAGCATGATCCGAGAG41.9417475728155338No Hit
ACACAAAATCCGCGTGACCAAAAGAAACAC41.9417475728155338No Hit
GTGATGGACACCAAGCTAACAGAAACCC41.9417475728155338No Hit
TATCAGCATGGATTCTGGCGCCA31.4563106796116505No Hit
CAGAGAAAGATCATGGCCCCA31.4563106796116505No Hit
CTCCTCATAGACCGATGACT31.4563106796116505No Hit
AAATCCGCGTGACCAAAAGAAAC31.4563106796116505No Hit
ACCAGATTCATGTATCCTCTAGACACTCC31.4563106796116505No Hit
TGATATTCCATCTGAGCTGAGCCTG31.4563106796116505No Hit
CAGCACAGCCGTTGGTAACC31.4563106796116505No Hit
TAAGTAAACAGCACAGCCGTTGGTAACCCC31.4563106796116505No Hit
ACTTGACATCACAGTTGGCACAGTTCTC31.4563106796116505No Hit
CCTCAGAGGCCTTTAGCAATATAAACACC31.4563106796116505No Hit
TTAGCCGTGATGATCTCTCCT31.4563106796116505No Hit
ATAGTCGCAATCATCATGAGTATAAAGAAG31.4563106796116505No Hit
ACTATGTAGCCTGAAGAAGAG31.4563106796116505No Hit
TTGAAGCACAGGTAGCATGATCCGAGAG31.4563106796116505No Hit
CTTAGCCGTGATGATCT20.9708737864077669No Hit
ATCAACTGAGCAAACATCTAAGC20.9708737864077669No Hit
GTTGTCGCTCTTGCACTCGAATGTCTC20.9708737864077669No Hit
AAGTTTGAAGCACAGGTAGCATGATCCG20.9708737864077669No Hit
CCTTAGTGGCTGGCTTCAAATGCAC20.9708737864077669No Hit
CAAGAAACTTCACTAAGTCCC20.9708737864077669No Hit
TGTATTGGCAAAACAACTCATCTCC20.9708737864077669No Hit
TCAAAGAGATTCTGAGTGGTTGAAAAAA20.9708737864077669No Hit
CGCACTTAGAAACTCGGTATGCCCTTCCA20.9708737864077669No Hit
TCCTCTAGACACTCCTGATCC20.9708737864077669No Hit
TAATTTGACTCCAATTG20.9708737864077669No Hit
GCGTGACCAAAAGAAACGAACAAAATTTC20.9708737864077669No Hit
AGATCTTGATTGTCGCTACTGAG20.9708737864077669No Hit
ATAGACCGATGACTTTCCTAGCC20.9708737864077669No Hit
CGTTGTCGCTCTTGCACTCGA20.9708737864077669No Hit
CCTCATAGACCGATGACTTTCC20.9708737864077669No Hit
CTTGTATTGGCAAAACAACTCATCTCC20.9708737864077669No Hit
CACAGTTGGCACAGTTCTCCTTGATCA20.9708737864077669No Hit
TAACAACCTCTGCATAACA10.48543689320388345No Hit
TCAGCATGGATTCTGGCGCCAG10.48543689320388345No Hit
GATTCATGTATCCTCTAGACACTC10.48543689320388345No Hit
ATGAATCCATATTCCCTACCTC10.48543689320388345No Hit
TTTCTGGCATCTCCAACTG10.48543689320388345No Hit
TAGACCGATGACTTTCCTAGC10.48543689320388345No Hit
TTGTATTGGCAAAACAACTCATCTCC10.48543689320388345No Hit
TCATAGACCGATGACTTTCCT10.48543689320388345No Hit
AGGCGGCGCTGTGATGGACACC10.48543689320388345No Hit
ATCGAAGGTCGTCTTTCCTCC10.48543689320388345No Hit
AACCCTGTTTGGAGGCAAGAGATG10.48543689320388345No Hit
TTCTCAACCTCCTTTTTCCCATTCACACAA10.48543689320388345No Hit
CCTCAGAGGCCTTTAGCAATATAAACAC10.48543689320388345No Hit
TCCCAGAGAAAGATCATGGCC10.48543689320388345No Hit
TTGTCGCTCTTGCACTCGAATGTCTC10.48543689320388345No Hit
CAGAACAATCAAGACAGTAACCAACAT10.48543689320388345No Hit
TTCGATGGCTGCCTTA10.48543689320388345No Hit
GAATCTCCGTATCTGCAATTTTCTGTGTTT10.48543689320388345No Hit
GTACAGATGCTAGGCCAC10.48543689320388345No Hit
TTGACATCACAGTTGGCAC10.48543689320388345No Hit
AACCCTGTTTGGAGGCAAG10.48543689320388345No Hit
AAAGAAACGAACAAAATTTCAAAAT10.48543689320388345No Hit
ATGCCTGAGTAAGTCAGATCTAA10.48543689320388345No Hit
CGCGTTGTCGCTCTTGCACTC10.48543689320388345No Hit
AGATTCATGTATCCTCTAGACACTCC10.48543689320388345No Hit
GGCGGCGCTGTGATGGACAC10.48543689320388345No Hit
ATTTGTCAACCTCAAACA10.48543689320388345No Hit
CATCTCAAAACAAAAGTCTGTT10.48543689320388345No Hit
TTTAAGTAAACAGCACAGCCGTT10.48543689320388345No Hit
TCCATTGCAGTCTGAGAC10.48543689320388345No Hit
TGGTTGAGGCGGCGCTGTGATGGACAC10.48543689320388345No Hit
CTCCTTTCACATTAG10.48543689320388345No Hit
ATCACGCAATCTTGC10.48543689320388345No Hit
ATGCCATCTCTTTCCACC10.48543689320388345No Hit
GTGACCAAAAGAAACGAACAAAATTTCAAA10.48543689320388345No Hit
TCCTAGCCAGATCTTGATTGTCGCCACT10.48543689320388345No Hit
CATAGACCGATGACTTTCCTAGCC10.48543689320388345No Hit
ACACCAAGCTAACAGAAACCCCA10.48543689320388345No Hit
GAAGCACAGGTAGCATGATCCGAG10.48543689320388345No Hit
AGGTACAGGCTTGCCTCT10.48543689320388345No Hit
AGGTGCAGCCTCTCCAGAGCCGATGGTCA10.48543689320388345No Hit
GTTGTCGCTCTTGCACTCGAATGTCT10.48543689320388345No Hit
AACCCTGTTTGGAGGCAAGAGATGGATCC10.48543689320388345No Hit
CACAGGCTCTTCCTATTACTCTA10.48543689320388345No Hit
CATTAGCCCTGTAAGACC10.48543689320388345No Hit
CAAGCCGTGATGATC10.48543689320388345No Hit
TAAAAATTTCAAAAT10.48543689320388345No Hit
TGACCCTATGACAATTGCCCA10.48543689320388345No Hit
CGGCGCTGTGATGGACACCAAGCTAAC10.48543689320388345No Hit
TAGGTCGCGTTGTCGCTCTTGCACTCGAA10.48543689320388345No Hit
CACAAAATCCGCGTGACCAAAAGAAACAC10.48543689320388345No Hit
AGCTAACAGAAACCCCATCAA10.48543689320388345No Hit
TGATATTCCATCAGAGCTGAGCCTG10.48543689320388345No Hit
ACAGCCGTTGGTAACCCCTCCT10.48543689320388345No Hit
CCTGATAATTTAGCCACTAAATAACC10.48543689320388345No Hit
CAGTCTGAGACTCCTTCAATC10.48543689320388345No Hit
CTAAGTCCCAGAGAAAGATCATGGCCCC10.48543689320388345No Hit
TCCAACTGCATCGTCTTT10.48543689320388345No Hit
TTAGAAACTCGGTATGCCC10.48543689320388345No Hit
TCCGCGTGACCAAAAGAAACGAACAAAA10.48543689320388345No Hit
TTTGGAGGCAAGAGATGGATC10.48543689320388345No Hit
CATCTATAGAACCACATTAAA10.48543689320388345No Hit
GCACTTAGAAACTCGGTATGCCCTT10.48543689320388345No Hit
CAATGGATCCAACTG10.48543689320388345No Hit
CATTCACACACTTGACATCAC10.48543689320388345No Hit
AGGTCGCGTTGTCGCTCTTGCACTCGAAT10.48543689320388345No Hit
CTTAGCCGTGATGATCTCTCC10.48543689320388345No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)