##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	S1_Sudafrica.Phlebovirus_segment_M.15-35nt.new.forward-mapped.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	426
Sequences flagged as poor quality	0
Sequence length	20-35
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.0	37.0	37.0	37.0	37.0	37.0
2	36.18544600938967	37.0	37.0	37.0	37.0	37.0
3	35.76995305164319	37.0	37.0	37.0	37.0	37.0
4	36.028169014084504	37.0	37.0	37.0	37.0	37.0
5	36.262910798122064	37.0	37.0	37.0	37.0	37.0
6	36.286384976525824	37.0	37.0	37.0	37.0	37.0
7	36.352112676056336	37.0	37.0	37.0	37.0	37.0
8	36.59154929577465	37.0	37.0	37.0	37.0	37.0
9	36.08920187793427	37.0	37.0	37.0	37.0	37.0
10	36.563380281690144	37.0	37.0	37.0	37.0	37.0
11	36.34272300469483	37.0	37.0	37.0	37.0	37.0
12	36.35680751173709	37.0	37.0	37.0	37.0	37.0
13	36.497652582159624	37.0	37.0	37.0	37.0	37.0
14	36.629107981220656	37.0	37.0	37.0	37.0	37.0
15	36.36619718309859	37.0	37.0	37.0	37.0	37.0
16	36.478873239436616	37.0	37.0	37.0	37.0	37.0
17	36.056338028169016	37.0	37.0	37.0	37.0	37.0
18	36.74178403755869	37.0	37.0	37.0	37.0	37.0
19	36.46948356807512	37.0	37.0	37.0	37.0	37.0
20	36.32394366197183	37.0	37.0	37.0	37.0	37.0
21	36.47016706443914	37.0	37.0	37.0	37.0	37.0
22	36.17258883248731	37.0	37.0	37.0	37.0	37.0
23	36.675257731958766	37.0	37.0	37.0	37.0	37.0
24	36.67204301075269	37.0	37.0	37.0	37.0	37.0
25	36.12737127371274	37.0	37.0	37.0	37.0	37.0
26	36.61666666666667	37.0	37.0	37.0	37.0	37.0
27	36.56896551724138	37.0	37.0	37.0	37.0	37.0
28	36.67065868263473	37.0	37.0	37.0	37.0	37.0
29	36.22875816993464	37.0	37.0	37.0	37.0	37.0
30	36.51449275362319	37.0	37.0	37.0	37.0	37.0
31	36.75100401606426	37.0	37.0	37.0	37.0	37.0
32	36.39366515837104	37.0	37.0	37.0	37.0	37.0
33	36.57225433526011	37.0	37.0	37.0	37.0	37.0
34	36.68852459016394	37.0	37.0	37.0	37.0	37.0
35	37.0	NaN	NaN	NaN	NaN	NaN
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
2104	1	0.0
2104	2	1.026315789473685
2104	3	0.9473684210526301
2104	4	0.0
2104	5	0.0
2104	6	0.0
2104	7	1.0
2104	8	0.3157894736842124
2104	9	1.0
2104	10	-11.684210526315788
2104	11	0.3157894736842124
2104	12	0.3157894736842124
2104	13	0.0
2104	14	0.6315789473684177
2104	15	0.6315789473684177
2104	16	-11.526315789473685
2104	17	0.0
2104	18	-11.684210526315788
2104	19	0.0
2104	20	0.6842105263157876
2104	21	1.6052631578947398
2104	22	-20.42105263157895
2104	23	4.868421052631582
2104	24	6.815789473684209
2104	25	6.815789473684209
2104	26	7.131578947368421
2104	27	8.105263157894736
2104	28	9.736842105263158
2104	29	15.605263157894736
2104	30	15.236842105263158
2104	31	17.18421052631579
2104	32	18.5
2104	33	23.684210526315788
2104	34	28.236842105263158
2104	35	32.131578947368425
2110	1	0.0
2110	2	1.026315789473685
2110	3	0.9473684210526301
2110	4	0.0
2110	5	0.0
2110	6	0.0
2110	7	1.0
2110	8	0.3157894736842124
2110	9	1.0
2110	10	0.3157894736842124
2110	11	0.3157894736842124
2110	12	0.3157894736842124
2110	13	0.0
2110	14	0.6315789473684177
2110	15	0.6315789473684177
2110	16	0.47368421052631504
2110	17	0.0
2110	18	0.3157894736842124
2110	19	0.0
2110	20	0.6842105263157876
2110	21	1.6052631578947398
2110	22	5.578947368421051
2110	23	4.868421052631582
2110	24	6.815789473684209
2110	25	6.815789473684209
2110	26	7.131578947368421
2110	27	8.105263157894736
2110	28	9.736842105263158
2110	29	-21.394736842105264
2110	30	-21.763157894736842
2110	31	-19.81578947368421
2110	32	-18.5
2110	33	-13.31578947368421
2110	34	-8.763157894736842
2110	35	-4.868421052631579
2111	1	0.0
2111	2	1.026315789473685
2111	3	0.9473684210526301
2111	4	0.0
2111	5	0.0
2111	6	0.0
2111	7	1.0
2111	8	0.3157894736842124
2111	9	1.0
2111	10	0.3157894736842124
2111	11	0.3157894736842124
2111	12	0.3157894736842124
2111	13	0.0
2111	14	0.6315789473684177
2111	15	0.6315789473684177
2111	16	0.47368421052631504
2111	17	0.0
2111	18	0.3157894736842124
2111	19	0.0
2111	20	0.6842105263157876
2111	21	1.6052631578947398
2111	22	5.578947368421051
2111	23	4.868421052631582
2111	24	6.815789473684209
2111	25	6.815789473684209
2111	26	7.131578947368421
2111	27	8.105263157894736
2111	28	9.736842105263158
2111	29	15.605263157894736
2111	30	15.236842105263158
2111	31	17.18421052631579
2111	32	18.5
2111	33	-13.31578947368421
2111	34	-8.763157894736842
2111	35	-4.868421052631579
2118	1	0.0
2118	2	1.026315789473685
2118	3	0.9473684210526301
2118	4	0.0
2118	5	0.0
2118	6	0.0
2118	7	1.0
2118	8	0.3157894736842124
2118	9	1.0
2118	10	0.3157894736842124
2118	11	0.3157894736842124
2118	12	0.3157894736842124
2118	13	0.0
2118	14	0.6315789473684177
2118	15	0.6315789473684177
2118	16	0.47368421052631504
2118	17	0.0
2118	18	0.3157894736842124
2118	19	0.0
2118	20	0.6842105263157876
2118	21	1.6052631578947398
2118	22	5.578947368421051
2118	23	4.868421052631582
2118	24	6.815789473684209
2118	25	6.815789473684209
2118	26	7.131578947368421
2118	27	8.105263157894736
2118	28	9.736842105263158
2118	29	15.605263157894736
2118	30	15.236842105263158
2118	31	5.184210526315791
2118	32	18.5
2118	33	23.684210526315788
2118	34	28.236842105263158
2118	35	32.131578947368425
2125	1	0.0
2125	2	1.026315789473685
2125	3	0.9473684210526301
2125	4	0.0
2125	5	0.0
2125	6	0.0
2125	7	1.0
2125	8	0.3157894736842124
2125	9	1.0
2125	10	0.3157894736842124
2125	11	0.3157894736842124
2125	12	0.3157894736842124
2125	13	0.0
2125	14	0.6315789473684177
2125	15	0.6315789473684177
2125	16	0.47368421052631504
2125	17	0.0
2125	18	0.3157894736842124
2125	19	0.0
2125	20	0.6842105263157876
2125	21	1.6052631578947398
2125	22	-31.42105263157895
2125	23	-32.13157894736842
2125	24	-30.18421052631579
2125	25	-30.18421052631579
2125	26	-29.86842105263158
2125	27	-28.894736842105264
2125	28	-27.263157894736842
2125	29	-21.394736842105264
2125	30	-21.763157894736842
2125	31	-19.81578947368421
2125	32	-18.5
2125	33	-13.31578947368421
2125	34	-8.763157894736842
2125	35	-4.868421052631579
2126	1	0.0
2126	2	1.026315789473685
2126	3	-11.05263157894737
2126	4	0.0
2126	5	0.0
2126	6	0.0
2126	7	-25.0
2126	8	0.3157894736842124
2126	9	1.0
2126	10	0.3157894736842124
2126	11	0.3157894736842124
2126	12	0.3157894736842124
2126	13	0.0
2126	14	0.6315789473684177
2126	15	-11.368421052631582
2126	16	0.47368421052631504
2126	17	0.0
2126	18	0.3157894736842124
2126	19	0.0
2126	20	0.6842105263157876
2126	21	1.6052631578947398
2126	22	-31.42105263157895
2126	23	-32.13157894736842
2126	24	-30.18421052631579
2126	25	-30.18421052631579
2126	26	-29.86842105263158
2126	27	-28.894736842105264
2126	28	-27.263157894736842
2126	29	-21.394736842105264
2126	30	-21.763157894736842
2126	31	-19.81578947368421
2126	32	-18.5
2126	33	-13.31578947368421
2126	34	-8.763157894736842
2126	35	-4.868421052631579
2133	1	0.0
2133	2	1.026315789473685
2133	3	0.9473684210526301
2133	4	0.0
2133	5	0.0
2133	6	0.0
2133	7	1.0
2133	8	0.3157894736842124
2133	9	1.0
2133	10	0.3157894736842124
2133	11	0.3157894736842124
2133	12	0.3157894736842124
2133	13	0.0
2133	14	0.6315789473684177
2133	15	0.6315789473684177
2133	16	0.47368421052631504
2133	17	0.0
2133	18	0.3157894736842124
2133	19	0.0
2133	20	0.6842105263157876
2133	21	1.6052631578947398
2133	22	5.578947368421051
2133	23	4.868421052631582
2133	24	-30.18421052631579
2133	25	-30.18421052631579
2133	26	-29.86842105263158
2133	27	-28.894736842105264
2133	28	-27.263157894736842
2133	29	-21.394736842105264
2133	30	-21.763157894736842
2133	31	-19.81578947368421
2133	32	-18.5
2133	33	-13.31578947368421
2133	34	-8.763157894736842
2133	35	-4.868421052631579
2135	1	0.0
2135	2	1.026315789473685
2135	3	0.9473684210526301
2135	4	0.0
2135	5	0.0
2135	6	0.0
2135	7	1.0
2135	8	0.3157894736842124
2135	9	1.0
2135	10	0.3157894736842124
2135	11	-11.684210526315788
2135	12	-11.684210526315788
2135	13	0.0
2135	14	-11.368421052631582
2135	15	0.6315789473684177
2135	16	0.47368421052631504
2135	17	0.0
2135	18	0.3157894736842124
2135	19	0.0
2135	20	0.6842105263157876
2135	21	-10.39473684210526
2135	22	5.578947368421051
2135	23	4.868421052631582
2135	24	6.815789473684209
2135	25	6.815789473684209
2135	26	7.131578947368421
2135	27	-28.894736842105264
2135	28	-27.263157894736842
2135	29	-21.394736842105264
2135	30	-21.763157894736842
2135	31	-19.81578947368421
2135	32	-18.5
2135	33	-13.31578947368421
2135	34	-8.763157894736842
2135	35	-4.868421052631579
2141	1	0.0
2141	2	1.026315789473685
2141	3	0.9473684210526301
2141	4	0.0
2141	5	0.0
2141	6	0.0
2141	7	1.0
2141	8	0.3157894736842124
2141	9	1.0
2141	10	0.3157894736842124
2141	11	0.3157894736842124
2141	12	0.3157894736842124
2141	13	0.0
2141	14	0.6315789473684177
2141	15	0.6315789473684177
2141	16	0.47368421052631504
2141	17	0.0
2141	18	0.3157894736842124
2141	19	0.0
2141	20	0.6842105263157876
2141	21	1.6052631578947398
2141	22	5.578947368421051
2141	23	4.868421052631582
2141	24	6.815789473684209
2141	25	6.815789473684209
2141	26	7.131578947368421
2141	27	8.105263157894736
2141	28	9.736842105263158
2141	29	3.6052631578947363
2141	30	15.236842105263158
2141	31	17.18421052631579
2141	32	18.5
2141	33	-13.31578947368421
2141	34	-8.763157894736842
2141	35	-4.868421052631579
2145	1	0.0
2145	2	1.026315789473685
2145	3	0.9473684210526301
2145	4	0.0
2145	5	0.0
2145	6	0.0
2145	7	1.0
2145	8	0.3157894736842124
2145	9	1.0
2145	10	0.3157894736842124
2145	11	0.3157894736842124
2145	12	0.3157894736842124
2145	13	0.0
2145	14	0.6315789473684177
2145	15	-11.368421052631582
2145	16	0.47368421052631504
2145	17	0.0
2145	18	0.3157894736842124
2145	19	0.0
2145	20	0.6842105263157876
2145	21	1.6052631578947398
2145	22	5.578947368421051
2145	23	4.868421052631582
2145	24	6.815789473684209
2145	25	6.815789473684209
2145	26	7.131578947368421
2145	27	8.105263157894736
2145	28	9.736842105263158
2145	29	15.605263157894736
2145	30	15.236842105263158
2145	31	17.18421052631579
2145	32	18.5
2145	33	23.684210526315788
2145	34	28.236842105263158
2145	35	-4.868421052631579
2149	1	0.0
2149	2	1.026315789473685
2149	3	0.9473684210526301
2149	4	0.0
2149	5	0.0
2149	6	0.0
2149	7	1.0
2149	8	0.3157894736842124
2149	9	1.0
2149	10	0.3157894736842124
2149	11	0.3157894736842124
2149	12	0.3157894736842124
2149	13	0.0
2149	14	0.6315789473684177
2149	15	0.6315789473684177
2149	16	0.47368421052631504
2149	17	0.0
2149	18	0.3157894736842124
2149	19	0.0
2149	20	-25.315789473684212
2149	21	1.6052631578947398
2149	22	5.578947368421051
2149	23	4.868421052631582
2149	24	6.815789473684209
2149	25	6.815789473684209
2149	26	7.131578947368421
2149	27	8.105263157894736
2149	28	9.736842105263158
2149	29	15.605263157894736
2149	30	15.236842105263158
2149	31	17.18421052631579
2149	32	18.5
2149	33	23.684210526315788
2149	34	-8.763157894736842
2149	35	-4.868421052631579
2155	1	0.0
2155	2	-11.973684210526315
2155	3	0.9473684210526301
2155	4	0.0
2155	5	0.0
2155	6	0.0
2155	7	1.0
2155	8	0.3157894736842124
2155	9	1.0
2155	10	0.3157894736842124
2155	11	0.3157894736842124
2155	12	0.3157894736842124
2155	13	0.0
2155	14	0.6315789473684177
2155	15	0.6315789473684177
2155	16	0.47368421052631504
2155	17	0.0
2155	18	0.3157894736842124
2155	19	0.0
2155	20	0.6842105263157876
2155	21	1.6052631578947398
2155	22	5.578947368421051
2155	23	4.868421052631582
2155	24	6.815789473684209
2155	25	6.815789473684209
2155	26	7.131578947368421
2155	27	8.105263157894736
2155	28	9.736842105263158
2155	29	15.605263157894736
2155	30	15.236842105263158
2155	31	17.18421052631579
2155	32	18.5
2155	33	23.684210526315788
2155	34	28.236842105263158
2155	35	-4.868421052631579
2161	1	0.0
2161	2	1.026315789473685
2161	3	0.9473684210526301
2161	4	0.0
2161	5	0.0
2161	6	0.0
2161	7	1.0
2161	8	0.3157894736842124
2161	9	1.0
2161	10	0.3157894736842124
2161	11	0.3157894736842124
2161	12	0.3157894736842124
2161	13	0.0
2161	14	0.6315789473684177
2161	15	0.6315789473684177
2161	16	0.47368421052631504
2161	17	0.0
2161	18	0.3157894736842124
2161	19	0.0
2161	20	0.6842105263157876
2161	21	1.6052631578947398
2161	22	-6.421052631578949
2161	23	4.868421052631582
2161	24	6.815789473684209
2161	25	6.815789473684209
2161	26	7.131578947368421
2161	27	8.105263157894736
2161	28	-27.263157894736842
2161	29	-21.394736842105264
2161	30	-21.763157894736842
2161	31	-19.81578947368421
2161	32	-18.5
2161	33	-13.31578947368421
2161	34	-8.763157894736842
2161	35	-4.868421052631579
2164	1	0.0
2164	2	1.026315789473685
2164	3	0.9473684210526301
2164	4	0.0
2164	5	0.0
2164	6	0.0
2164	7	1.0
2164	8	0.3157894736842124
2164	9	1.0
2164	10	0.3157894736842124
2164	11	0.3157894736842124
2164	12	0.3157894736842124
2164	13	0.0
2164	14	0.6315789473684177
2164	15	0.6315789473684177
2164	16	0.47368421052631504
2164	17	0.0
2164	18	0.3157894736842124
2164	19	0.0
2164	20	0.6842105263157876
2164	21	1.6052631578947398
2164	22	5.578947368421051
2164	23	4.868421052631582
2164	24	6.815789473684209
2164	25	6.815789473684209
2164	26	7.131578947368421
2164	27	8.105263157894736
2164	28	9.736842105263158
2164	29	15.605263157894736
2164	30	15.236842105263158
2164	31	-19.81578947368421
2164	32	-18.5
2164	33	-13.31578947368421
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2256	12	0.3157894736842124
2256	13	0.0
2256	14	0.6315789473684177
2256	15	0.6315789473684177
2256	16	0.47368421052631504
2256	17	0.0
2256	18	0.3157894736842124
2256	19	0.0
2256	20	0.6842105263157876
2256	21	1.6052631578947398
2256	22	5.578947368421051
2256	23	4.868421052631582
2256	24	6.815789473684209
2256	25	6.815789473684209
2256	26	7.131578947368421
2256	27	8.105263157894736
2256	28	9.736842105263158
2256	29	15.605263157894736
2256	30	15.236842105263158
2256	31	17.18421052631579
2256	32	18.5
2256	33	23.684210526315788
2256	34	28.236842105263158
2256	35	-4.868421052631579
2262	1	0.0
2262	2	1.026315789473685
2262	3	0.9473684210526301
2262	4	0.0
2262	5	0.0
2262	6	0.0
2262	7	1.0
2262	8	-11.684210526315788
2262	9	1.0
2262	10	0.3157894736842124
2262	11	0.3157894736842124
2262	12	0.3157894736842124
2262	13	0.0
2262	14	0.6315789473684177
2262	15	0.6315789473684177
2262	16	0.47368421052631504
2262	17	0.0
2262	18	0.3157894736842124
2262	19	0.0
2262	20	0.6842105263157876
2262	21	-35.39473684210526
2262	22	-31.42105263157895
2262	23	-32.13157894736842
2262	24	-30.18421052631579
2262	25	-30.18421052631579
2262	26	-29.86842105263158
2262	27	-28.894736842105264
2262	28	-27.263157894736842
2262	29	-21.394736842105264
2262	30	-21.763157894736842
2262	31	-19.81578947368421
2262	32	-18.5
2262	33	-13.31578947368421
2262	34	-8.763157894736842
2262	35	-4.868421052631579
2263	1	0.0
2263	2	1.026315789473685
2263	3	0.9473684210526301
2263	4	0.0
2263	5	0.0
2263	6	0.0
2263	7	1.0
2263	8	0.3157894736842124
2263	9	1.0
2263	10	0.3157894736842124
2263	11	0.3157894736842124
2263	12	0.3157894736842124
2263	13	0.0
2263	14	0.6315789473684177
2263	15	0.6315789473684177
2263	16	0.47368421052631504
2263	17	0.0
2263	18	0.3157894736842124
2263	19	0.0
2263	20	0.6842105263157876
2263	21	1.6052631578947398
2263	22	5.578947368421051
2263	23	-32.13157894736842
2263	24	-30.18421052631579
2263	25	-30.18421052631579
2263	26	-29.86842105263158
2263	27	-28.894736842105264
2263	28	-27.263157894736842
2263	29	-21.394736842105264
2263	30	-21.763157894736842
2263	31	-19.81578947368421
2263	32	-18.5
2263	33	-13.31578947368421
2263	34	-8.763157894736842
2263	35	-4.868421052631579
2267	1	0.0
2267	2	1.026315789473685
2267	3	0.9473684210526301
2267	4	0.0
2267	5	0.0
2267	6	0.0
2267	7	1.0
2267	8	0.3157894736842124
2267	9	1.0
2267	10	0.3157894736842124
2267	11	0.3157894736842124
2267	12	0.3157894736842124
2267	13	0.0
2267	14	0.6315789473684177
2267	15	0.6315789473684177
2267	16	0.47368421052631504
2267	17	0.0
2267	18	0.3157894736842124
2267	19	0.0
2267	20	0.6842105263157876
2267	21	1.6052631578947398
2267	22	5.578947368421051
2267	23	4.868421052631582
2267	24	6.815789473684209
2267	25	6.815789473684209
2267	26	7.131578947368421
2267	27	8.105263157894736
2267	28	9.736842105263158
2267	29	15.605263157894736
2267	30	15.236842105263158
2267	31	17.18421052631579
2267	32	18.5
2267	33	23.684210526315788
2267	34	-8.763157894736842
2267	35	-4.868421052631579
2270	1	0.0
2270	2	1.026315789473685
2270	3	0.9473684210526301
2270	4	0.0
2270	5	0.0
2270	6	0.0
2270	7	1.0
2270	8	0.3157894736842124
2270	9	1.0
2270	10	0.3157894736842124
2270	11	0.3157894736842124
2270	12	0.3157894736842124
2270	13	0.0
2270	14	0.6315789473684177
2270	15	0.6315789473684177
2270	16	0.47368421052631504
2270	17	0.0
2270	18	0.3157894736842124
2270	19	0.0
2270	20	0.6842105263157876
2270	21	1.6052631578947398
2270	22	5.578947368421051
2270	23	4.868421052631582
2270	24	6.815789473684209
2270	25	6.815789473684209
2270	26	7.131578947368421
2270	27	8.105263157894736
2270	28	9.736842105263158
2270	29	15.605263157894736
2270	30	15.236842105263158
2270	31	17.18421052631579
2270	32	18.5
2270	33	23.684210526315788
2270	34	-8.763157894736842
2270	35	-4.868421052631579
2273	1	0.0
2273	2	1.026315789473685
2273	3	0.9473684210526301
2273	4	0.0
2273	5	0.0
2273	6	0.0
2273	7	1.0
2273	8	0.3157894736842124
2273	9	1.0
2273	10	0.3157894736842124
2273	11	0.3157894736842124
2273	12	0.3157894736842124
2273	13	0.0
2273	14	0.6315789473684177
2273	15	0.6315789473684177
2273	16	0.47368421052631504
2273	17	0.0
2273	18	0.3157894736842124
2273	19	0.0
2273	20	0.6842105263157876
2273	21	1.6052631578947398
2273	22	5.578947368421051
2273	23	4.868421052631582
2273	24	6.815789473684209
2273	25	6.815789473684209
2273	26	7.131578947368421
2273	27	8.105263157894736
2273	28	9.736842105263158
2273	29	15.605263157894736
2273	30	3.2368421052631575
2273	31	5.184210526315791
2273	32	18.5
2273	33	-13.31578947368421
2273	34	-8.763157894736842
2273	35	-4.868421052631579
2274	1	0.0
2274	2	1.026315789473685
2274	3	0.9473684210526301
2274	4	0.0
2274	5	0.0
2274	6	0.0
2274	7	1.0
2274	8	0.3157894736842124
2274	9	1.0
2274	10	0.3157894736842124
2274	11	0.3157894736842124
2274	12	0.3157894736842124
2274	13	0.0
2274	14	0.6315789473684177
2274	15	0.6315789473684177
2274	16	0.47368421052631504
2274	17	0.0
2274	18	0.3157894736842124
2274	19	0.0
2274	20	0.6842105263157876
2274	21	1.6052631578947398
2274	22	5.578947368421051
2274	23	4.868421052631582
2274	24	-30.18421052631579
2274	25	-30.18421052631579
2274	26	-29.86842105263158
2274	27	-28.894736842105264
2274	28	-27.263157894736842
2274	29	-21.394736842105264
2274	30	-21.763157894736842
2274	31	-19.81578947368421
2274	32	-18.5
2274	33	-13.31578947368421
2274	34	-8.763157894736842
2274	35	-4.868421052631579
2277	1	0.0
2277	2	1.026315789473685
2277	3	0.9473684210526301
2277	4	0.0
2277	5	0.0
2277	6	0.0
2277	7	1.0
2277	8	0.3157894736842124
2277	9	1.0
2277	10	0.3157894736842124
2277	11	0.3157894736842124
2277	12	0.3157894736842124
2277	13	0.0
2277	14	0.6315789473684177
2277	15	0.6315789473684177
2277	16	0.47368421052631504
2277	17	0.0
2277	18	0.3157894736842124
2277	19	0.0
2277	20	0.6842105263157876
2277	21	1.6052631578947398
2277	22	5.578947368421051
2277	23	4.868421052631582
2277	24	6.815789473684209
2277	25	6.815789473684209
2277	26	7.131578947368421
2277	27	8.105263157894736
2277	28	9.736842105263158
2277	29	-21.394736842105264
2277	30	-21.763157894736842
2277	31	-19.81578947368421
2277	32	-18.5
2277	33	-13.31578947368421
2277	34	-8.763157894736842
2277	35	-4.868421052631579
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	1.0
29	2.0
30	1.0
31	5.0
32	7.0
33	6.0
34	20.0
35	36.0
36	80.0
37	268.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	6.103286384976526	30.751173708920188	34.27230046948357	28.87323943661972
2	13.411764705882353	35.05882352941177	30.352941176470587	21.176470588235293
3	19.483568075117372	30.751173708920188	30.28169014084507	19.483568075117372
4	22.535211267605636	24.88262910798122	26.995305164319248	25.586854460093893
5	15.96244131455399	31.455399061032864	26.76056338028169	25.821596244131456
6	28.638497652582164	27.464788732394368	22.065727699530516	21.830985915492956
7	31.220657276995308	19.014084507042252	22.065727699530516	27.699530516431924
8	25.352112676056336	30.51643192488263	13.849765258215962	30.28169014084507
9	25.352112676056336	32.3943661971831	25.11737089201878	17.136150234741784
10	25.821596244131456	27.699530516431924	26.76056338028169	19.718309859154928
11	29.107981220657276	32.3943661971831	23.708920187793428	14.788732394366196
12	22.535211267605636	26.291079812206576	21.36150234741784	29.812206572769952
13	15.492957746478872	28.638497652582164	30.751173708920188	25.11737089201878
14	23.474178403755868	29.577464788732392	13.849765258215962	33.098591549295776
15	19.248826291079812	40.61032863849765	16.666666666666664	23.474178403755868
16	24.88262910798122	30.046948356807512	21.36150234741784	23.708920187793428
17	17.370892018779344	26.76056338028169	27.230046948356808	28.638497652582164
18	16.666666666666664	36.61971830985916	25.352112676056336	21.36150234741784
19	19.248826291079812	27.464788732394368	24.178403755868544	29.107981220657276
20	19.248826291079812	31.690140845070424	20.89201877934272	28.169014084507044
21	14.319809069212411	31.980906921241047	19.09307875894988	34.60620525059666
22	13.451776649746192	41.11675126903553	14.720812182741117	30.710659898477154
23	16.49484536082474	21.649484536082475	26.54639175257732	35.30927835051546
24	18.548387096774192	28.49462365591398	28.49462365591398	24.462365591397848
25	15.176151761517614	31.978319783197833	28.455284552845526	24.390243902439025
26	16.666666666666664	29.72222222222222	20.27777777777778	33.33333333333333
27	14.942528735632186	35.63218390804598	15.517241379310345	33.90804597701149
28	7.18562874251497	35.62874251497006	16.16766467065868	41.01796407185629
29	16.013071895424837	42.810457516339866	21.895424836601308	19.28104575163399
30	15.942028985507244	47.10144927536232	12.681159420289855	24.27536231884058
31	12.851405622489958	22.08835341365462	43.77510040160642	21.285140562248998
32	11.312217194570136	23.52941176470588	21.266968325791854	43.89140271493213
33	21.38728323699422	28.32369942196532	23.699421965317917	26.589595375722542
34	12.295081967213115	43.44262295081967	13.934426229508196	30.327868852459016
35	2.0408163265306123	40.816326530612244	16.3265306122449	40.816326530612244
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.5
19	1.0
20	1.0
21	1.5
22	1.5
23	1.0
24	1.0
25	1.0
26	1.5
27	1.5
28	1.5
29	2.0
30	3.5
31	4.5
32	5.0
33	10.5
34	14.0
35	13.5
36	22.5
37	45.0
38	60.5
39	59.0
40	48.0
41	50.5
42	58.0
43	80.5
44	106.5
45	118.0
46	115.0
47	82.0
48	60.5
49	61.5
50	63.0
51	56.5
52	43.5
53	39.0
54	39.0
55	29.0
56	17.0
57	9.5
58	10.0
59	20.0
60	27.5
61	22.5
62	14.5
63	7.0
64	2.0
65	0.5
66	0.0
67	0.0
68	0.5
69	1.0
70	1.0
71	1.0
72	1.0
73	0.5
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.2347417840375587
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
20	7.0
21	25.0
22	6.0
23	16.0
24	3.0
25	9.0
26	12.0
27	14.0
28	28.0
29	30.0
30	27.0
31	28.0
32	48.0
33	51.0
34	73.0
35	49.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.74178403755869
#Duplication Level	Percentage of deduplicated	Percentage of total
1	48.64864864864865	16.901408450704224
2	13.513513513513514	9.389671361502346
3	13.513513513513514	14.084507042253522
4	4.72972972972973	6.572769953051644
5	5.405405405405405	9.389671361502346
6	1.3513513513513513	2.8169014084507045
7	2.027027027027027	4.929577464788732
8	4.72972972972973	13.145539906103288
9	1.3513513513513513	4.225352112676056
>10	4.72972972972973	18.544600938967136
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGTCTGGATGAATCCATATTCCCTACCTCATCTG	13	3.051643192488263	No Hit
TAAGTTTGAAGCACAGGTAGCATGATCCG	12	2.8169014084507045	No Hit
AAATCCGCGTGACCAAAAGAAACGAACAAAAT	12	2.8169014084507045	No Hit
ATGTAGCCTGAAGAAGAGCCATCTTGACAATGGA	11	2.5821596244131455	No Hit
TAAGTTTGAAGCACAGGTAGCATGATCCGAGAG	11	2.5821596244131455	No Hit
CTCAGAGGCCTTTAGCAATATAAACACCACCGCC	10	2.3474178403755865	No Hit
TAACCAACATGCTGATCAGCCCCTTAGATATCCA	10	2.3474178403755865	No Hit
CATGACCCATTGCACATTCTCAACCTCCTTTTTTC	9	2.112676056338028	No Hit
ACTTTGTAGGTCGCGTTGTCGCTCTTGCACTCG	9	2.112676056338028	No Hit
TTAGCAATATAAACACCACCGCCAGAACAATCAA	8	1.8779342723004695	No Hit
CAACATGCTGATCAGCCCCTTAGATATCCA	8	1.8779342723004695	No Hit
TTCAAATGCACGTACTCTAATCTTCCATTCAA	8	1.8779342723004695	No Hit
CTTTAAGTAAACAGCACAGCCGTTGGTAACCC	8	1.8779342723004695	No Hit
CCTCCACAACAGGATGATCCCATCTCAAAACAAAA	8	1.8779342723004695	No Hit
AATCCGCGTGACCAAAAGAAACGAACAAAATTTCT	8	1.8779342723004695	No Hit
TGCAAGTGACTGTTCCTGAATCTCCGTATCT	8	1.8779342723004695	No Hit
TTGCAGGTGCAGCCTCTCCAGAGCCGATGGTCA	7	1.643192488262911	No Hit
AAATCCGCGTGACCAAAAGAAACGAACAAAA	7	1.643192488262911	No Hit
AGTTTGCATAAGAGTAGCACTTCAGGTTGATCC	7	1.643192488262911	No Hit
TAGGTCGCGTTGTCGCTCTTGCACTCGAAT	6	1.4084507042253522	No Hit
CCCAATGATGGTTGAGGCGGCGCTGTGATGGACAC	6	1.4084507042253522	No Hit
CCTGAGTAAGTCAGATCTAAAGATTCAACCACACA	5	1.1737089201877933	No Hit
TTCACACAAGCTTTCTTCTGAACTCCATCAAC	5	1.1737089201877933	No Hit
TGAAGCACAGGTAGCATGATCCGAGAG	5	1.1737089201877933	No Hit
GCGGCGCTGTGATGGACACCAAGCTAAC	5	1.1737089201877933	No Hit
CCGCGTGACCAAAAGAAACGAACAAAATTTCAAA	5	1.1737089201877933	No Hit
AGGTCGCGTTGTCGCTCTTGCACTCGAATGTCTC	5	1.1737089201877933	No Hit
TAACATCCTGATAGACTAACAACCTCTGCATAAC	5	1.1737089201877933	No Hit
TCTTAGCCGTGATGATCTCTCCTTTCAC	5	1.1737089201877933	No Hit
GTTCTGTAGGTTTCTGAGTGGCTTGG	4	0.9389671361502347	No Hit
CTGATTCAATGCCATCTCTTTCCACC	4	0.9389671361502347	No Hit
ACACAAAATCCGCGTGACCAAAAGAAACAC	4	0.9389671361502347	No Hit
GTGATGGACACCAAGCTAACAGAAACCC	4	0.9389671361502347	No Hit
TTCTCAACCTCCTTTTTCCCATTCACACAAG	4	0.9389671361502347	No Hit
ATCCGCGTGACCAAAAGAAACGAACAAAATTTC	4	0.9389671361502347	No Hit
CAGAGGCCTTTAGCAATATAAACACCACCGCC	4	0.9389671361502347	No Hit
AATATAAACACCACCGCCAGAACAATCAAGAC	3	0.7042253521126761	No Hit
TATCAGCATGGATTCTGGCGCCA	3	0.7042253521126761	No Hit
CAGAGAAAGATCATGGCCCCA	3	0.7042253521126761	No Hit
CTCCTCATAGACCGATGACT	3	0.7042253521126761	No Hit
AAATCCGCGTGACCAAAAGAAAC	3	0.7042253521126761	No Hit
ACCAGATTCATGTATCCTCTAGACACTCC	3	0.7042253521126761	No Hit
TTTGAAGCACAGGTAGCATGATCCGAGAG	3	0.7042253521126761	No Hit
AACTTCACTAAGTCCCAGAGAAAGATCATGGCC	3	0.7042253521126761	No Hit
TGATATTCCATCTGAGCTGAGCCTG	3	0.7042253521126761	No Hit
CAGCACAGCCGTTGGTAACC	3	0.7042253521126761	No Hit
TAAGTAAACAGCACAGCCGTTGGTAACCCC	3	0.7042253521126761	No Hit
ACTTGACATCACAGTTGGCACAGTTCTC	3	0.7042253521126761	No Hit
CTCAGAGGCCTTTAGCAATATAAACACCACC	3	0.7042253521126761	No Hit
TCAAGACAGTAACCAACATGCTGATCAGCCCCTT	3	0.7042253521126761	No Hit
CTAAGTCCCAGAGAAAGATCATGGCCCCAGATA	3	0.7042253521126761	No Hit
CCTCAGAGGCCTTTAGCAATATAAACACC	3	0.7042253521126761	No Hit
TTAGCCGTGATGATCTCTCCT	3	0.7042253521126761	No Hit
ATAGTCGCAATCATCATGAGTATAAAGAAG	3	0.7042253521126761	No Hit
ACTATGTAGCCTGAAGAAGAG	3	0.7042253521126761	No Hit
TTGAAGCACAGGTAGCATGATCCGAGAG	3	0.7042253521126761	No Hit
GACTTTCCTAGCCAGATCTTGATTGTCGCCACT	2	0.4694835680751174	No Hit
TATGGATACAATGCACAGAACTAGTAAGTGTGA	2	0.4694835680751174	No Hit
ATCAACTGAGCAAACATCTAAGC	2	0.4694835680751174	No Hit
CTTTGTCTTAGCCGTGATGATCTCTCCTTTC	2	0.4694835680751174	No Hit
GTTGTCGCTCTTGCACTCGAATGTCTC	2	0.4694835680751174	No Hit
AAGTTTGAAGCACAGGTAGCATGATCCG	2	0.4694835680751174	No Hit
CCTTAGTGGCTGGCTTCAAATGCAC	2	0.4694835680751174	No Hit
CAAGAAACTTCACTAAGTCCC	2	0.4694835680751174	No Hit
TCAAAGAGATTCTGAGTGGTTGAAAAAA	2	0.4694835680751174	No Hit
CGCACTTAGAAACTCGGTATGCCCTTCCA	2	0.4694835680751174	No Hit
GCGTGACCAAAAGAAACGAACAAAATTTC	2	0.4694835680751174	No Hit
AGATCTTGATTGTCGCTACTGAG	2	0.4694835680751174	No Hit
ATAGACCGATGACTTTCCTAGCC	2	0.4694835680751174	No Hit
CGTTGTCGCTCTTGCACTCGA	2	0.4694835680751174	No Hit
TTTCCCAATGATGGTTGAGGCGGCGCTGTGATGGA	2	0.4694835680751174	No Hit
TAAGTTTGAAGCACAGGTAGCATGATACGAGAG	2	0.4694835680751174	No Hit
CCTCATAGACCGATGACTTTCC	2	0.4694835680751174	No Hit
CTTGTATTGGCAAAACAACTCATCTCC	2	0.4694835680751174	No Hit
TAGGTCGCGTTGTCGCTCTTGCACTCGAATGTCTC	2	0.4694835680751174	No Hit
CACAGTTGGCACAGTTCTCCTTGATCA	2	0.4694835680751174	No Hit
TCAGCATGGATTCTGGCGCCAG	1	0.2347417840375587	No Hit
ACATCCTGATAGACTAACAACCTCTGCATAAC	1	0.2347417840375587	No Hit
CCAATGATGGTTGAGGCGGCGCTGTGATGGACAC	1	0.2347417840375587	No Hit
ATGAATCCATATTCCCTACCTC	1	0.2347417840375587	No Hit
TAGACCGATGACTTTCCTAGC	1	0.2347417840375587	No Hit
AAAACAACTCATCTCCATTGCAGTCTGAGACTCCC	1	0.2347417840375587	No Hit
CCCAATGATGGTTGAGGCGGCGCTGTGATGGA	1	0.2347417840375587	No Hit
TTGTATTGGCAAAACAACTCATCTCC	1	0.2347417840375587	No Hit
ATCGAAGGTCGTCTTTCCTCC	1	0.2347417840375587	No Hit
TAAGTTTGAAGCACAGGTAGCATGATCCGAG	1	0.2347417840375587	No Hit
AACCCTGTTTGGAGGCAAGAGATG	1	0.2347417840375587	No Hit
TTCTCAACCTCCTTTTTCCCATTCACACAA	1	0.2347417840375587	No Hit
TTCTGTGTTTCCCTTTAGATTCTGAGTGTTA	1	0.2347417840375587	No Hit
CCTCAGAGGCCTTTAGCAATATAAACAC	1	0.2347417840375587	No Hit
TAGGTCGCGTTGTCGCTCTTGCACTCGAATGTCT	1	0.2347417840375587	No Hit
TCCCAGAGAAAGATCATGGCC	1	0.2347417840375587	No Hit
ACTTCACTAAGTCCCAGAGAAAGATCATGGCC	1	0.2347417840375587	No Hit
TTGTCGCTCTTGCACTCGAATGTCTC	1	0.2347417840375587	No Hit
CAGAACAATCAAGACAGTAACCAACAT	1	0.2347417840375587	No Hit
TGTATTGGCAAAACAACTCATCTCC	1	0.2347417840375587	No Hit
GAATCTCCGTATCTGCAATTTTCTGTGTTT	1	0.2347417840375587	No Hit
ATCCGCGTGACCAAAAGAAACGAACAAAATTT	1	0.2347417840375587	No Hit
AAAGAAACGAACAAAATTTCAAAAT	1	0.2347417840375587	No Hit
ATGCCTGAGTAAGTCAGATCTAA	1	0.2347417840375587	No Hit
CGCGTTGTCGCTCTTGCACTC	1	0.2347417840375587	No Hit
GGCGGCGCTGTGATGGACAC	1	0.2347417840375587	No Hit
CTTCACTAAGTCCCAGAGAAAGATCATGGCC	1	0.2347417840375587	No Hit
CATCTCAAAACAAAAGTCTGTT	1	0.2347417840375587	No Hit
TTTAAGTAAACAGCACAGCCGTT	1	0.2347417840375587	No Hit
CTCAGAGGCCTTTAGCAATATAAACACCACCGCCA	1	0.2347417840375587	No Hit
TCCTCTAGACACTCCTGATCC	1	0.2347417840375587	No Hit
ACTTTCCTAGCCAGATCTTGATTGTCGCCACT	1	0.2347417840375587	No Hit
TGGTTGAGGCGGCGCTGTGATGGACAC	1	0.2347417840375587	No Hit
AATTTCAAAATTCAATTCCTATTCAGTCATCTATA	1	0.2347417840375587	No Hit
GTGACCAAAAGAAACGAACAAAATTTCAAA	1	0.2347417840375587	No Hit
TCACACAAGCTTTCTTCTGAACTCCATCAAC	1	0.2347417840375587	No Hit
TCCTAGCCAGATCTTGATTGTCGCCACT	1	0.2347417840375587	No Hit
CATAGACCGATGACTTTCCTAGCC	1	0.2347417840375587	No Hit
ACACCAAGCTAACAGAAACCCCA	1	0.2347417840375587	No Hit
GAAGCACAGGTAGCATGATCCGAG	1	0.2347417840375587	No Hit
ACAAAATCCGCGTGACCAAAAGAAACGAACAAAAA	1	0.2347417840375587	No Hit
AGGTGCAGCCTCTCCAGAGCCGATGGTCA	1	0.2347417840375587	No Hit
GTTGTCGCTCTTGCACTCGAATGTCT	1	0.2347417840375587	No Hit
AACCCTGTTTGGAGGCAAGAGATGGATCC	1	0.2347417840375587	No Hit
CACAGGCTCTTCCTATTACTCTA	1	0.2347417840375587	No Hit
CNTCCACAACAGGATGATCCCATCTCAAAACAAAA	1	0.2347417840375587	No Hit
ATTTCAAAATTCAATTCCTATTCAGTCATCTATAG	1	0.2347417840375587	No Hit
TGACCCTATGACAATTGCCCA	1	0.2347417840375587	No Hit
CGGCGCTGTGATGGACACCAAGCTAAC	1	0.2347417840375587	No Hit
TAGGTCGCGTTGTCGCTCTTGCACTCGAA	1	0.2347417840375587	No Hit
TTGAAGGTGCAGCCTCTCCAGAGCCGATGGTCA	1	0.2347417840375587	No Hit
CACAAAATCCGCGTGACCAAAAGAAACAC	1	0.2347417840375587	No Hit
AGCTAACAGAAACCCCATCAA	1	0.2347417840375587	No Hit
CCTCCACAACAGGATGATCCCATCTCAAAACAAA	1	0.2347417840375587	No Hit
TGATATTCCATCAGAGCTGAGCCTG	1	0.2347417840375587	No Hit
ACAGCCGTTGGTAACCCCTCCT	1	0.2347417840375587	No Hit
CCCAATGATGGTTGAGGCGGCGCTGTGATGGACAA	1	0.2347417840375587	No Hit
CCTGATAATTTAGCCACTAAATAACC	1	0.2347417840375587	No Hit
CAGTCTGAGACTCCTTCAATC	1	0.2347417840375587	No Hit
CTAAGTCCCAGAGAAAGATCATGGCCCC	1	0.2347417840375587	No Hit
AAGACCAAGAGGCGCACTTAGAAACTCGGTATGCC	1	0.2347417840375587	No Hit
TCCGCGTGACCAAAAGAAACGAACAAAA	1	0.2347417840375587	No Hit
GTTTGCATAAGAGTAGCACTTCAGGTTGATCC	1	0.2347417840375587	No Hit
CCACAACAGGATGATCCCATCTCAAAACAAAAGTC	1	0.2347417840375587	No Hit
TTTGGAGGCAAGAGATGGATC	1	0.2347417840375587	No Hit
CATCTATAGAACCACATTAAA	1	0.2347417840375587	No Hit
GCACTTAGAAACTCGGTATGCCCTT	1	0.2347417840375587	No Hit
AAAACCGCGTGACCAAAAGAAACGAACAAAAT	1	0.2347417840375587	No Hit
CATTCACACACTTGACATCAC	1	0.2347417840375587	No Hit
AGGTCGCGTTGTCGCTCTTGCACTCGAAT	1	0.2347417840375587	No Hit
CTTAGCCGTGATGATCTCTCC	1	0.2347417840375587	No Hit
AAGTTTGAAGCACAGGTAGCATGATCCGAGAG	1	0.2347417840375587	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
