FastQCFastQC Report
Mon 28 Nov 2022
S1_Sudafrica.Phlebovirus_segment_M.15-35nt.new.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS1_Sudafrica.Phlebovirus_segment_M.15-35nt.new.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences226
Sequences flagged as poor quality0
Sequence length20-35
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCGGCTCTGGAGAGGCTGCACCTGCAAGAAGAC156.637168141592921No Hit
TAACAATTGATGACTCCCCGAAGAACATGACTTTC125.3097345132743365No Hit
CATAACAATTGATGACTCCCCGAAGAACATGACT93.982300884955752No Hit
TGACCACCAAATATAAGGACCCTGAAATGAA83.5398230088495577No Hit
TTTGACCATCGGCTCTGGAGAGGCTGCACCTGCAA83.5398230088495577No Hit
GGCTAGGAAAGTCATCGGTCTATGAGGAGAAGAAA73.0973451327433628No Hit
ACTCTAGTTAGGCCTCCTTCAAGAC73.0973451327433628No Hit
TAACAATTGATGACTCCCCGA73.0973451327433628No Hit
CCTCTGAGGCTCTCCTAAAGAAAATAAAAA73.0973451327433628No Hit
ACATTGGGAGAAATTCAGTGTCCGACAGTTGG62.6548672566371683No Hit
TCAAGGAGAACTGTGCCAACTGTGATGTCA62.6548672566371683No Hit
ACAGTCACTTGCACAACTTCCGAAACAACG62.6548672566371683No Hit
AATATAAGGACCCTGAAATGAAAGATGAGAA62.6548672566371683No Hit
ACAGGGCTAATGTGAAAGGAGAGATCATC52.2123893805309733No Hit
TAACAATTGATGACTCCCCGAAGAACATGACTT52.2123893805309733No Hit
CTCTGAGGCTCTCCTAAAGAAAATAAAAGAA52.2123893805309733No Hit
CTTTAGATCTGACTTACTCAGGCATGATTCCC52.2123893805309733No Hit
CTCTGAGGCTCTCCTAAAGAAAATAAT41.7699115044247788No Hit
CTCCATAACAATTGATGACTCCCCGAAGAA41.7699115044247788No Hit
TAGATCAAGATATGGCTTCACTCAAACTAACAA41.7699115044247788No Hit
TTACTAGTTCTGTGCATTGTA31.3274336283185841No Hit
TACGGAGATTCAGGAACAGTCACTTGCACTAAA31.3274336283185841No Hit
CTCTGAGGCTCTCCTAAAGAAAATAATATAATC31.3274336283185841No Hit
TGGCCTAGCATCTGTACTGAAGACAC31.3274336283185841No Hit
CAGGGCTAATGTGAAAGGAGAGATCATCA31.3274336283185841No Hit
ATTGATGACTCCCCGAAGAACATGACTTTCCT31.3274336283185841No Hit
AATTGATGACTCCCCGAAGAA31.3274336283185841No Hit
GATGACTCCCCGAAGAACATGACTTTCCTTGA31.3274336283185841No Hit
AGTTAGGCCTCCTTCAAGACAGATTCCT20.8849557522123894No Hit
AAGATGAGACATTCGAGTGCAAGAGCGAC20.8849557522123894No Hit
ATACCTGAACCAGCTCATGAG20.8849557522123894No Hit
ACTTAGCTCATGACTGTATAACACCACAATC20.8849557522123894No Hit
ACGGAGATTCAGGAACAGTCA20.8849557522123894No Hit
AATAGACTTTCACCGAGAGGCTCACGCAA20.8849557522123894No Hit
GAAAATTGCAGATACGGAGATTCAGGA20.8849557522123894No Hit
CAATCTGGCTGCAAAGGAAATGGATCC20.8849557522123894No Hit
CAGGGCTAATGTAAAAGGAGAGATCATCA20.8849557522123894No Hit
GAATAGACTTTCACCGAGAGGCTCACGCAA20.8849557522123894No Hit
CGAACGTCTACTGCTCCACCA20.8849557522123894No Hit
CTCTGAGGCTCTCCTAAAGAAAATAAAAA20.8849557522123894No Hit
TTGGTCACGCGGATTTTGTGTTT20.8849557522123894No Hit
TTGACCATCGGCTCTGGAGAGGCTGCACCTGCAA20.8849557522123894No Hit
TGACTCCCCGAAGAACATGAC20.8849557522123894No Hit
ACTTCCGAAACAACGAGACTG10.4424778761061947No Hit
TATAAGGACCCTGAAATGAAA10.4424778761061947No Hit
ATATAAGGACCCTGAAATGAAAGATGAGAA10.4424778761061947No Hit
ATTGATGACTCCCCGAAGAAC10.4424778761061947No Hit
CGAAGAACATGACTTTCCTTGATCAGCTAAA10.4424778761061947No Hit
TTGATGACTCCCCGAAGAACA10.4424778761061947No Hit
TATTGCTAAAGGCCTCTGAGG10.4424778761061947No Hit
ATATACCTGAACCAGCTCATGAG10.4424778761061947No Hit
AAAACAATTGATGACTCCCCGAAGAA10.4424778761061947No Hit
AAGGGGAAGGAGGGGTTACCAACGGC10.4424778761061947No Hit
AACAATTGATGACTCCCCGAA10.4424778761061947No Hit
AGCCTGTACCTAGATTCAACCACTCAGAA10.4424778761061947No Hit
GCCTGTACCTAGATTCAACCACTCAGAA10.4424778761061947No Hit
TGACTCCCCGAAGAACATGACTTTCCTTGA10.4424778761061947No Hit
ATAACAATTGATGACTCCCCGAAGAA10.4424778761061947No Hit
TATACCTGAACCAGCTCATGAG10.4424778761061947No Hit
ATAGTAAGGCAGCCATCGAAC10.4424778761061947No Hit
ATACGGAGATTCAGGAACAGTCA10.4424778761061947No Hit
TTAGATCTGACTTACTCAGGCATGATTCCC10.4424778761061947No Hit
ATGACTCCCCGAAGAACATGACTTTCCTTGA10.4424778761061947No Hit
TGTGAAATGCTCAGGAGGAAAGAC10.4424778761061947No Hit
TCAAGATATGGCTTCACTCAAACTAACAAAAA10.4424778761061947No Hit
ACAATTGATGACTCCCCGAAG10.4424778761061947No Hit
AATTACCTGTAAATAATGTAAA10.4424778761061947No Hit
CCTCTGAGGCTCTCCTAAAGAAAATAATATAATC10.4424778761061947No Hit
TGAGAATAGACTTTCACCGAG10.4424778761061947No Hit
AATGGGTCATGGTAGAAGACA10.4424778761061947No Hit
TGACCATCGGCTCTGGAGAGGCTGCACCTGCAA10.4424778761061947No Hit
CATCGGCTCTGGAGAGGCTGCATCTGCAAGAAGAC10.4424778761061947No Hit
ATAACAATTGATGACTCCCCGAAGAACATGACT10.4424778761061947No Hit
TTACTATATACCTGAACCAGC10.4424778761061947No Hit
CTGAAACCCTCATGATGAACACCA10.4424778761061947No Hit
AGTCACTTGCACAACTTCCGAAACAACG10.4424778761061947No Hit
ATAAGGACCCTGAAATGAAA10.4424778761061947No Hit
AACAATTGATGACTCCCCGAAGAACATGACTTTC10.4424778761061947No Hit
AAATATAAGGACCCTGAAATGAAAG10.4424778761061947No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)