FastQCFastQC Report
Mon 28 Nov 2022
S1_Sudafrica.Phlebovirus_segment_M.15-35nt.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS1_Sudafrica.Phlebovirus_segment_M.15-35nt.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161
Sequences flagged as poor quality0
Sequence length15-30
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATTGATGACTCCCCGA127.453416149068323No Hit
ACTCTAGTTAGGCCTCCTTCAAGAC95.590062111801243No Hit
TAACAATTGATGACTCCCCGA74.3478260869565215No Hit
CCTCTGAGGCTCTCCTAAAGAAAATAAAAA74.3478260869565215No Hit
TCAAGGAGAACTGTGCCAACTGTGATGTCA63.7267080745341614No Hit
ACAGTCACTTGCACAACTTCCGAAACAACG63.7267080745341614No Hit
ACAGGGCTAATGTGAAAGGAGAGATCATC53.1055900621118013No Hit
CTCTGAGGCTCTCCTAAAGAAAATAAT42.484472049689441No Hit
ACGTGCATTTGAAGC42.484472049689441No Hit
CTCCATAACAATTGATGACTCCCCGAAGAA42.484472049689441No Hit
TTACTAGTTCTGTGCATTGTA31.8633540372670807No Hit
TGGCCTAGCATCTGTACTGAAGACAC31.8633540372670807No Hit
AAGATGAGACATTCGAGTGCAAGAGCGAC31.8633540372670807No Hit
CAGGGCTAATGTGAAAGGAGAGATCATCA31.8633540372670807No Hit
TAAGGACCCTGAAATGAA31.8633540372670807No Hit
AATTGATGACTCCCCGAAGAA31.8633540372670807No Hit
AGTTAGGCCTCCTTCAAGACAGATTCCT21.2422360248447204No Hit
AGATATGGCTTCACTC21.2422360248447204No Hit
TATTGCTAAAGGCCTCTGAGG21.2422360248447204No Hit
ATACCTGAACCAGCTCATGAG21.2422360248447204No Hit
ACGGAGATTCAGGAACAGTCA21.2422360248447204No Hit
AATAGACTTTCACCGAGAGGCTCACGCAA21.2422360248447204No Hit
GAAAATTGCAGATACGGAGATTCAGGA21.2422360248447204No Hit
CAATCTGGCTGCAAAGGAAATGGATCC21.2422360248447204No Hit
CAGGGCTAATGTAAAAGGAGAGATCATCA21.2422360248447204No Hit
GAATAGACTTTCACCGAGAGGCTCACGCAA21.2422360248447204No Hit
CGAACGTCTACTGCTCCACCA21.2422360248447204No Hit
CTCTGAGGCTCTCCTAAAGAAAATAAAAA21.2422360248447204No Hit
ACGAGACTGGTTGTT21.2422360248447204No Hit
TTGGTCACGCGGATTTTGTGTTT21.2422360248447204No Hit
TGACTCCCCGAAGAACATGAC21.2422360248447204No Hit
TCGAACTGGCGCCAG21.2422360248447204No Hit
ATGGTAGAAGACAAA10.6211180124223602No Hit
ACTTCCGAAACAACGAGACTG10.6211180124223602No Hit
TATAAGGACCCTGAAATGAAA10.6211180124223602No Hit
ATATAAGGACCCTGAAATGAAAGATGAGAA10.6211180124223602No Hit
ATTGATGACTCCCCGAAGAAC10.6211180124223602No Hit
ATCTGGCTGCAAAGGAAATG10.6211180124223602No Hit
TAGGCCTCCTTCAAG10.6211180124223602No Hit
TTGATGACTCCCCGAAGAACA10.6211180124223602No Hit
TATACCTGAACCAGCTC10.6211180124223602No Hit
ATATACCTGAACCAGCTCATGAG10.6211180124223602No Hit
AAAACAATTGATGACTCCCCGAAGAA10.6211180124223602No Hit
AAGGGGAAGGAGGGGTTACCAACGGC10.6211180124223602No Hit
AACAATTGATGACTCCCCGAA10.6211180124223602No Hit
AGCCTGTACCTAGATTCAACCACTCAGAA10.6211180124223602No Hit
TGGTAGAAGACAAAGTGAT10.6211180124223602No Hit
GCCTGTACCTAGATTCAACCACTCAGAA10.6211180124223602No Hit
TGACTCCCCGAAGAACATGACTTTCCTTGA10.6211180124223602No Hit
AGATGAGGAAAAGAA10.6211180124223602No Hit
ATAACAATTGATGACTCCCCGAAGAA10.6211180124223602No Hit
TGAGACATTCGAGTGC10.6211180124223602No Hit
GCTGAGGAAAAGAAA10.6211180124223602No Hit
TATACCTGAACCAGCTCATGAG10.6211180124223602No Hit
ATAGTAAGGCAGCCATCGAAC10.6211180124223602No Hit
CGGATCATGCTACCT10.6211180124223602No Hit
AAAGGAGAGATCATCACG10.6211180124223602No Hit
CAATTGATGACTCCCCGAAG10.6211180124223602No Hit
ATACGGAGATTCAGGAACAGTCA10.6211180124223602No Hit
TTAGATCTGACTTACTCAGGCATGATTCCC10.6211180124223602No Hit
AATCTGGCTGCAAAG10.6211180124223602No Hit
CTATAGATCTGACTTACTCAGGCATGATT10.6211180124223602No Hit
TGTGAAATGCTCAGGAGGAAAGAC10.6211180124223602No Hit
AAGAGACAGTCACCACT10.6211180124223602No Hit
ACAATTGATGACTCCCCGAAG10.6211180124223602No Hit
ATTGATGACTCCCCG10.6211180124223602No Hit
TGAGAATAGACTTTCACCG10.6211180124223602No Hit
AATTACCTGTAAATAATGTAAA10.6211180124223602No Hit
TGAGAATAGACTTTCACCGAG10.6211180124223602No Hit
AATGGGTCATGGTAGAAGACA10.6211180124223602No Hit
TAGGCCTCCTTCAAGACA10.6211180124223602No Hit
ATGATCAATTCCGCAGATTCTTGC10.6211180124223602No Hit
TTACTATATACCTGAACCAGC10.6211180124223602No Hit
CTGAAACCCTCATGATGAACACCA10.6211180124223602No Hit
AGTCACTTGCACAACTTCCGAAACAACG10.6211180124223602No Hit
ATAAGGACCCTGAAATGAAA10.6211180124223602No Hit
TGGTAGAAGACAAAG10.6211180124223602No Hit
AAATATAAGGACCCTGAAATGAAAG10.6211180124223602No Hit
AACCTGCCTTAAAGT10.6211180124223602No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)