FastQCFastQC Report
Fri 14 Oct 2022
S1.Phlebovirus_segment_M.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS1.Phlebovirus_segment_M.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3479
Sequences flagged as poor quality0
Sequence length20-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAAGTGATTGTTATGGGAGACATGACCACCAAATATAAGGACCCTGAA1083.104340327680368No Hit
GTGATTGTTATGGGAGACATGACCACCAAATATAAGGACCCTGAAATGAA671.925840758838747No Hit
AAGAAATGTAATGAGACATCGAACGTCTACTGCTCCACCAAGAAGACAGC551.5809140557631502No Hit
TTCCATTGACTCAACCTGCCTTAAAGTGTTAAAACAAGAGACAGTCACCA511.465938488071285No Hit
AGTGATCAAGGAGAACTGTGCCAACTGTGATGTCAAGTGTGTGAATGGAG501.4371945961483186No Hit
AGTGCAAGATTGCGTGATTGAGGGAGTCTCAGACTGCAATGGAGATGAGT471.3509629203794193No Hit
ACCAAATATAAGGACCCTGAAATGAAAGATGAGACATTCGAGTGCAAGAG461.322219028456453No Hit
GAAAATTGCAGATACGGAGATTCAGGAACAGTCACTTGCACAACTTCCGA451.2934751365334867No Hit
AGACAAAGTGATTGTTATGGGAGACATGACCACCAAATATAAGGACCCTG451.2934751365334867No Hit
TAGAAGACAAAGTGATTGTTATGGGAGACATGACCACCAAATATAAGGAC451.2934751365334867No Hit
TCGGCTCTGGAGAGGCTGCACCTGCAAGAAGACATTCAGCAGAATGGGTT441.2647312446105201No Hit
ATGGACTGAATGTTCCCAAGTAGAAGGAGGGGTTACCAACGGCTGTGCTG431.2359873526875538No Hit
ATCGGTCTATGAGGAGAAGAAAGTGGAGTGCAATGCCATCAGTGTCTGCA431.2359873526875538No Hit
TACTAGTTCTGTGCATTGTATCCATAATGATCAATTCCGCAGATTCTTGC421.2072434607645874No Hit
CTCAAACTAACAATATTATCAGTGGATCTGGTCTGTAAGCCATCTGCCAT411.178499568841621No Hit
GATACGGAGATTCAGGAACAGTCACTTGCACAACTTCCGAAACAACGAGA401.1497556769186548No Hit
CAGGGCTAATGTGAAAGGAGAGATCATCACGGCTAAGACAAAGAAAGAGG391.1210117849956884No Hit
GAAGAAAGCTTGTGTGAATGGAAAAAAGGAGGTTGAGAATGTGCAATGGG391.1210117849956884No Hit
CTTTCGTGTTGTGATTAGAACAACTCATGGTGAGTTCACACCTCAGGCAT391.1210117849956884No Hit
CACGGCTAAGACAAAGAAAGAGGCAAGCCTGTACCTAGATTCAACCACTC391.1210117849956884No Hit
ACAAAGTGCAAGATTGCGTGATTGAGGGAGTCTCAGACTGCAATGGAGAT391.1210117849956884No Hit
TGTTTACTTAAAGAGCTCATGCCTGTGGGTTAGGAAAGGAATTTCCAATC381.092267893072722No Hit
TGTTATGGGAGACATGACCACCAAATATAAGGACCCTGAAATGAAAGATG371.0635240011497558No Hit
CCACCAAATATAAGGACCCTGAAATGAAAGATGAGACATTCGAGTGCAAG371.0635240011497558No Hit
AAGTGCAAGATTGCGTGATTGAAGGAGTCTCAGACTGCAATGGAGATGAG361.0347801092267894No Hit
GATTGTTATGGGAGACATGACCACCAAATATAAGGACCCTGAAATGAAAG361.0347801092267894No Hit
GAAAGTGGAGTGCAATGCCATCAGTGTCTGCAACATGATAACTTGCACCT361.0347801092267894No Hit
TGTGCTGTTTACTTAAAGAGCTCATGCCTGTGGGTTAGGAAAGGAATTTC351.0060362173038229No Hit
TAAAGAGCTCATGCCTGTGGGTTAGGAAAGGAATTTCCAATCCTACTGGA351.0060362173038229No Hit
TGGGTCATGGTAGAAGACAAAGTGATTGTTATGGGAGACATGACCACCAA351.0060362173038229No Hit
CCATCAGTGTCTGCAACATGATAACTTGCACCTGGTGCTTTGACAACTGGATCAACCTGAAGTGCTACTC340.9772923253808565No Hit
ATGATCAATTCCGCAGATTCTTGCACTAACACTCAGAATCTAAAGGGAAA340.9772923253808565No Hit
AGTGATTGTTATGGGAGACATGACCACCAAATATAAGGACCCTGAAATGA330.9485484334578902No Hit
TTTGAGATGGGATCATCCTGTTGTGGAGGATGTTTGCACTGTGAAATGCT330.9485484334578902No Hit
AGAGTACGTGCATTTGAAGCCAGCCACTAAGGACGTCAGCTCCATAACAA330.9485484334578902No Hit
ATAATGATCAATTCCGCAGATTCTTGCACTAACACTCAGAATCTAAAGGG320.9198045415349237No Hit
AGGAAAGTCATCGGTCTATGAGGAAAAGAAAGTGGAGTGCAATGCCATCA320.9198045415349237No Hit
AGAAGACAAAGTGATTGTTATGGGAGACATGACCACCAAATATAAGGACCCTGAAATGAAAGATGAG320.9198045415349237No Hit
TGGGATGGACTGAATGTTCCCAAGTAGAAGGAGGGGTTACCAACGGCTGT320.9198045415349237No Hit
TGAAACTGAAACCCTCATGATGAACAATGAAGGTTATTTAGTGGCTAAAT310.8910606496119574No Hit
TTATACTCATGATGATTGCGACTATTGAAGTTGACTCTTCCATTGACTCA300.862316757688991No Hit
TCACGGCTAAGACAAAGAAAGAGGCAAGCCTGTACCTAGATTCAACCACT300.862316757688991No Hit
AGCCAAGGAAAGCAGCTTAGATGTTTGCTCAGTTGATGGAGTTCAGAAGAAAGCTTGTGTGAATGGAAA300.862316757688991No Hit
AAGTGATTGTTATGGGAGACATGACCACCAAATATAAGGACCCTGAAATGAAAGATGAGACATTCGA290.8335728657660249No Hit
TATAGATCAAGATATGGCTTCACTCAAACTAACAATATTATCAGTGGATC290.8335728657660249No Hit
TGTTTACTTAAAGAGCTCATGCCTGTGGGTTAGGAAAGGAATTTCCAATCCTACTGGAAGGGCATACCGA290.8335728657660249No Hit
TAGAGTAATAGGAAGAGCCTGTGGATCAGGAGTGTCTAGAGGATACATGA290.8335728657660249No Hit
TTTGAATGGAAGATTAGAGTACGTGCATTTGAAGCCAGCCACTAAGGACG290.8335728657660249No Hit
ACGTGCATTTGAAGCCAGCCACTAAGGACGTCAGCTCCATAACAATTGATGACTCCCCGAAGAA280.8048289738430584No Hit
TTCTTTATACTCATGATGATTGCGACTATTGAAGTTGACTCTTCCATTGA280.8048289738430584No Hit
ATGGATCCAAAACAACTGACATCCATTGTCAAGATGGCTCTTCTTCAGGC280.8048289738430584No Hit
TTCGAGTGCAAGAGCGACAACGCGACCTACAAAGTGCAAGATTGCGTGATTGAAGGAGTCTCAGACTGCA280.8048289738430584No Hit
ATTGATGACTCCCCGAAGAACATGACTTTCCTTGATCAGCTAAAAGACAT270.776085081920092No Hit
GTAGAAGACAAAGTGATTGTTATGGGAGACATGACCACCAAATATAAGGACCCTGAAATGAAAGATGA260.7473411899971256No Hit
CAACGAGACTGGTTGTTTCACCTCTGGGACAAACGTCTTGTTTGAGGTTG260.7473411899971256No Hit
GAGACAGTCACCACTTTTGACCATCGGCTCTGGAGAGGCTGCACCTGCAAGAAGACATTCAGCAGAATGGGTTT250.7185972980741593No Hit
AAGTGGAAGGAGGGGTTACCAACGGCTGTGCTGTTTACTTAAAGAGCTCA250.7185972980741593No Hit
GGATGTTTGCACTGTGAAATGCTCAGGAGGAAAGACGACCTTCGATTTGAATGGAAGATTAGAGTACGTGC240.6898534061511928No Hit
ATCCCCAAATGTGTCTAGTAGAGCAGACTCTAGAGCAGAAGCCAGAGTTTCAAGAGCTTTGCTAAAC240.6898534061511928No Hit
TCATCACGGCTAAGACAAAGAAAGAGGCAAGCCTGTACCTAGATTCAACC230.6611095142282265No Hit
TTAGTGAGTCCCTATGAAGTTACTTCTAGGGATCCTTTCCCCATCACAACTGAAACTGAAACCCTCA230.6611095142282265No Hit
CCATCGGCTCTGGAGAGGCTGCACCTGCAAGAAGACATTCAGCAGAATGGGTTTTGAGAAA220.6323656223052601No Hit
GTTCACACCTCAGGCATGGGGCAATCAGCTTGCCTTGGTGGAAAGAGATGGCATTG220.6323656223052601No Hit
CAATTGATGACTCCCCGAAGAACATGACTTTCCTTGATCAGCTAAAAGACATATGTA220.6323656223052601No Hit
ACCATCGGCTCTGGAGAGGCTGCACCTGCAAGAAGAC200.5748778384593274No Hit
GTGCAAGATTGCGTGATTGAAGGAGTCTCAGACTGCAATGGAGATGAGTTGTTTTGCCAATACAAGAA200.5748778384593274No Hit
ATAACAATTGATGACTCCCCGAAGAACATGACTTTCCTTGATCAGCTAAAAGAC200.5748778384593274No Hit
AAGTGATTGTTATGGGAGACATGACCACCAAATATAAGGACCCTGAAATGAAAGATGAGACATTCGAGTGC190.546133946536361No Hit
TCAAGGAGAACTGTGCCAACTGTGATGTCAAGTGTGTGAATGGAGTAGTGAGAATAGACTTTCACCG190.546133946536361No Hit
CACCAAATATAAGGACCCTGAAATGAAAGATGAGACATTCGAGTGCAAGAGCGACAACGCGAC190.546133946536361No Hit
AAGTGTTAAAACAAGAGACAGTCACCACTTTTGACCATCGGCTCTGGAGA190.546133946536361No Hit
GTGCCAACTGTGATGTCAAGTGTGTGAATGGAGTAGTGAGAATAGACTTTCACCGAGAG170.48864616269042827No Hit
GTGATGTCAAGTGTGTGAATGGAGTAGTGAGAATAGACTTTCACCGAGAA170.48864616269042827No Hit
CACCACTTTTGACCATCGGCTCTGGAGAGGCTGCACCTGCAAGAAGAC170.48864616269042827No Hit
ATCATCACGGCTAAGACAAAGAAAGAGGCAAGCCTGTACCTAGATTCAACC170.48864616269042827No Hit
AGACATGACCACCAAATATAAGGACCCTGAAATGAAAGATGAGACATTCGAG170.48864616269042827No Hit
TAAGGACCCTGAAATGAAAGATGAGACATTCGAGTGCAAGAGCGACAACGCGACCTAC160.4599022707674619No Hit
ACGCGACCTACAAAGTGCAAGATTGCGTGATTGAAGGAGTCTCAGACTGC160.4599022707674619No Hit
GAGATCATCACGGCTAAGACAAAGAAAGAGGCAAGCCTGTACCTAGATTCAACCACTCAGAA160.4599022707674619No Hit
AGACAAAGTGATTGTTATGGGAGACATGACCACCAAATATAAGGACCCTGAAATGAAAGATGA160.4599022707674619No Hit
CACGGCTAAGACAAAGAAAGAGGCAAGCCTGTACCTAGATTCAACCACT150.4311583788444955No Hit
AGTGATTGTTATGGGAGACATGACCACCAAATATAAGGACCCTGAAATGAAAGATGAGACAT150.4311583788444955No Hit
CATCGGCTCTGGAGAGGCTGCACCTGCAAGAAGAC150.4311583788444955No Hit
AAAGTGATTGTTATGGGAGACATGACCACCAAATATAAGGACCCTGAAATGAAAG140.4024144869215292No Hit
GATTGCGTGATTGAAGGAGTCTCAGACTGCAATGGAGATGAGTTGTTTTGCCAATA140.4024144869215292No Hit
ACAGTGATCAAGGAGAACTGTGCCAACTGTGATGTCAAGTGTGTGAATGGAGTAGTGAGAAT140.4024144869215292No Hit
AGACAGATTCCTGGAATAGCTAGAGCATCCAACAGATT130.3736705949985628No Hit
AACAATTGATGACTCCCCGAAGAACATGACTTTCCTTGATCAGCTAAAAGAC130.3736705949985628No Hit
TAACAATTGATGACTCCCCGAAGAACATGACTTTCCTTGATCAGCA130.3736705949985628No Hit
CTGTAAATAATGTAAATATTAGATTTTAACAAACTTGTACATATGTAAAAAA130.3736705949985628No Hit
TAACAATTGATGACTCCCCGAAGAACATGACTTTC120.3449267030755964No Hit
ATCAGGATGTTATAGCTGTCTGACTGGAGCCATAGTGG120.3449267030755964No Hit
CTGTTGGATTGGATATCTAAGGGGCTGATCAGCATGTTGGTTACTG120.3449267030755964No Hit
CTGTTGTGGAGGATGTTTGCACTGTGAAATGCTCAGGAGGAAAGACGAC110.31618281115263003No Hit
CACCACTTTTGACCATCGGCTCTGGAGAGGCTGCACCTGCAAGAAGACATTCAGC110.31618281115263003No Hit
TTTTGACCATCGGCTCTGGAGAGGCTGCACCTGCAAGAAGAC110.31618281115263003No Hit
CGGCTAAGACAAAGAAAGAGGCAAGCCTGTACCTAGATTCAA110.31618281115263003No Hit
GCAATTGTCATAGGGTCATTCTACCTCACAGTCTCCTGTCTAATGTGGTT110.31618281115263003No Hit
ATGTGTCTAGTAGAGCAGACTCTAGAGCAGAAGCCAGAGTTTCA100.2874389192296637No Hit
AATTGATGACTCCCCGAAGAACATGACTTTCCTTGATCAGCTAAAAGACA100.2874389192296637No Hit
ACAAATTCTATAGATCAAGATATGGCTTCACTCAAACTAACAAT100.2874389192296637No Hit
TAAAGGCCTCTGAGGCTCTCCCAAAGAAAATAATATAATC100.2874389192296637No Hit
AAAGTGATTGTTATGGGAGACATGACCACCAAATATAAGGACC100.2874389192296637No Hit
ATTGATGACTCCCCGAAGAACATGACTTTCCTTGATCAGCTAAAAG100.2874389192296637No Hit
CAATTGGAGTCAAATTACCTGTAAATAATGTAAAAAA90.25869502730669736No Hit
TGCAAGATTGCGTGATTGAAGGAGTCTCAGACTGCAATGGAGATGAGTTGT90.25869502730669736No Hit
TTGACCATCGGCTCTGGAGAGGCTGCACCTGCAAGA90.25869502730669736No Hit
CATAACAATTGATGACTCCCCGAAGAACATGACT90.25869502730669736No Hit
CCACCAAATATAAGGACCCTGAAATGAAAGATGAGACATTCGAGT90.25869502730669736No Hit
ATATAAGGACCCTGAAATGAAAGATGAGACATTCGAGTGCAAGAGCGACAACGCG90.25869502730669736No Hit
GACTCCCCGAAGAACATGACTTTCCTTGATCAGCTAAAAGACATATGTAGATTTAAC80.22995113538373094No Hit
TGACCACCAAATATAAGGACCCTGAAATGAA80.22995113538373094No Hit
GAAAATTGCAGATACGGAGATTCAGGAACAGTCACTTGCACAAC80.22995113538373094No Hit
ATAAGGACCCTGAAATGAAAGATGAGACATTCGAGTGCAAGAGCGACAACGCGACCT80.22995113538373094No Hit
CAAACAGGGTTAGACTTCATGTTCCAAGCCACTCAGAAACCTACAG80.22995113538373094No Hit
CCAAAGATAGTAAGGCAGCCATCGAACTGGCGCCAGAATCCATG80.22995113538373094No Hit
TCAGCTCCATAACAATTGATGACTCCCCGAAGAACATGACTTTCC80.22995113538373094No Hit
TTTGACCATCGGCTCTGGAGAGGCTGCACCTGCAA80.22995113538373094No Hit
AGGAGAACTGTGCCAACTGTGATGTCAAGTGTGTGAATGGAGTAGTGA70.2012072434607646No Hit
GGCTAGGAAAGTCATCGGTCTATGAGGAGAAGAAA70.2012072434607646No Hit
CCTCTGAGGCTCTCCTAAAGAAAATAAAAA70.2012072434607646No Hit
ACTCTAGTTAGGCCTCCTTCAAGAC70.2012072434607646No Hit
CCGAAGAACATGACTTTCCTTGATCAGCTAAAAGAA70.2012072434607646No Hit
TAACAATTGATGACTCCCCGA70.2012072434607646No Hit
AACAATTGATGACTCCCCGAAGAACATGACTTTCCTTGATCAGCTAAAAGACATATG60.1724633515377982No Hit
ACATTGGGAGAAATTCAGTGTCCGACAGTTGG60.1724633515377982No Hit
ACAGTCACTTGCACAACTTCCGAAACAACG60.1724633515377982No Hit
TAACAATTGATGACTCCCCGAAGAACATGACTTTCCTTGATCAGCT60.1724633515377982No Hit
AATATAAGGACCCTGAAATGAAAGATGAGAA60.1724633515377982No Hit
AAGACGATGCAGTTGGATCCATCTCTTGCCTCCAAACA60.1724633515377982No Hit
ATACGGAGATTCAGGAACAGTCACTTGCACAACTTCCGAAACAACGAGAC60.1724633515377982No Hit
TCAAGGAGAACTGTGCCAACTGTGATGTCA60.1724633515377982No Hit
AAAGTGGAGTGCAATGCCATCAGTGTCTGCAACATGATAACTTGCACCTG50.14371945961483185No Hit
GCCAAGGAAAGCAGCTTAGATGTTTGCTCAGTTGATGGAGTTCAGAAGAAAGCTTGTGTGAATGGAAA50.14371945961483185No Hit
TAACAATTGATGACTCCCCGAAGAACATGACTT50.14371945961483185No Hit
CTCTGAGGCTCTCCTAAAGAAAATAAAAGAA50.14371945961483185No Hit
ACAGGGCTAATGTGAAAGGAGAGATCATC50.14371945961483185No Hit
CTTTAGATCTGACTTACTCAGGCATGATTCCC50.14371945961483185No Hit
CTCTGAGGCTCTCCTAAAGAAAATAAT40.11497556769186547No Hit
CTCCATAACAATTGATGACTCCCCGAAGAA40.11497556769186547No Hit
CCATAACAATTGATGACTCCCCGAAGAACATGACTTTCC40.11497556769186547No Hit
CAATTGATGACTCCCCGAAGAACATGACTTTCCTTGATCAGCTAAAAG40.11497556769186547No Hit
TAGATCAAGATATGGCTTCACTCAAACTAACAA40.11497556769186547No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)