FastQCFastQC Report
Mon 28 Nov 2022
S1_Sudafrica.Phlebovirus_segment_S.15-35nt.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS1_Sudafrica.Phlebovirus_segment_S.15-35nt.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences571
Sequences flagged as poor quality0
Sequence length15-30
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCTGAACGCCTCTAA183.1523642732049035No Hit
CTGAAGATCCTGAGAAGAGAG132.276707530647986No Hit
TTCGGACATTTGGTCGAGCACTCGCT111.926444833625219No Hit
ATCGACGAAATGGCTGCCTTCACCTGGGAA111.926444833625219No Hit
TATAAGTCGAAGACAAAGCTTGAGAAGAG91.5761821366024518No Hit
CGCCTGAACGCCTCTAAG81.4010507880910683No Hit
GCCTGAACGCCTCTAA81.4010507880910683No Hit
CGCCTGAACGCCTCTA81.4010507880910683No Hit
GTTGAAGACAAAGCTTGAGAAGAGAGAGGA71.2259194395796849No Hit
ACATTTTTGGGACCATTTAAGAAGATACA71.2259194395796849No Hit
CCGCGGGATTACGCCTGAACGCCTCTAA71.2259194395796849No Hit
CCTCTAAGGCCGTATCCGTTCTAATCGT61.0507880910683012No Hit
TCCGCGGGATTACGCCTGAACGCCTCTAA61.0507880910683012No Hit
TCCTCAGCCAAGATGTCCACA61.0507880910683012No Hit
AAATTATCGACGAAATGGCTGCCTTCAC50.8756567425569177No Hit
GGAGGTTCTGAAGATCCTGAGAAGAGAG50.8756567425569177No Hit
TCCGCGGGATTACGCCTGAACGCCTCT50.8756567425569177No Hit
GACGCCTCCTCAGCCAAGATGTCCAC50.8756567425569177No Hit
CAGGTTCGGACATTTGGTCGAGCACTCGC50.8756567425569177No Hit
TGAAGATCCTGAGAAGAGAGAGTGGCAA50.8756567425569177No Hit
GCTCTGCTGGATGCCTATAAGGACTAATC50.8756567425569177No Hit
CGCCTGAACGCCTCT50.8756567425569177No Hit
AGAGTGGCAAAGAAAGATTGAAAACAAT50.8756567425569177No Hit
CGGGATTACGCCTGAACGCCTCTAAG50.8756567425569177No Hit
GAGACTTGCTGGTATGCGAACAAACACA50.8756567425569177No Hit
ATCCGCGGGATTACGCCTGAACGCCTC50.8756567425569177No Hit
TTACGCCTGAACGCCTCTAA40.7005253940455342No Hit
TTACGCCTGAACGCCTCTAAG40.7005253940455342No Hit
CCATCCGCGGGATTACGCCTGAACGC40.7005253940455342No Hit
GATTACGCCTGAACGCCTCT40.7005253940455342No Hit
ACGCCTGAACGCCTCTAA40.7005253940455342No Hit
CTCCTCAGCCAAGATGTCCAC40.7005253940455342No Hit
CCTGAACGCCTCTAA40.7005253940455342No Hit
ACGCTACCATCCGCGGGATTACGCCTGAA40.7005253940455342No Hit
TTCGGACATTTGGTCGAGCACTCGC40.7005253940455342No Hit
GGGATTACGCCTGAACGCCTCTAAGG40.7005253940455342No Hit
GTTCGGACATTTGGTCGAGCACTCGCT40.7005253940455342No Hit
CGGACATTTGGTCGAGCACTCG40.7005253940455342No Hit
ATCCGTTCTAATCGTAGTGGTGTAC40.7005253940455342No Hit
CCAAAGAGACTTGCTGGTATGCGAACAAAC40.7005253940455342No Hit
ACGCCTGAACGCCTCTAAG40.7005253940455342No Hit
GATTACGCCTGAACGCCTCTAA40.7005253940455342No Hit
ATTATCGACGAAATGGCTGCCTTCAC40.7005253940455342No Hit
TTTGGTCGAGCACTCGC30.5253940455341506No Hit
GAGAGTGGCAAAGAAAGATTGAAAACA30.5253940455341506No Hit
CGGGATTACGCCTGAACGCCTCT30.5253940455341506No Hit
ACGCCTCCTCAGCCAAGATGTCCACAGATG30.5253940455341506No Hit
GCGACGCTACCATCCGCGGGATTACGCCTG30.5253940455341506No Hit
ACCGCAGGTTCGGACATTTG30.5253940455341506No Hit
AAATGGCTGCCTTCACCTGGG30.5253940455341506No Hit
GACAGCTCTGGCTAAGGAGGTTCTGA30.5253940455341506No Hit
ATTATCGACGAAATGGCTGCCTTCACCTG30.5253940455341506No Hit
GAGAGTGGCAAAGAAAGATTGAAAAAAAA30.5253940455341506No Hit
ATCCGTTCTAATCGT30.5253940455341506No Hit
AGTGGCAAAGAAAGATTGAAAACAATAATG30.5253940455341506No Hit
TATCGACGAAATGGCTGCCTTCACCTG30.5253940455341506No Hit
AAATTATCGACGAAATGGCTGCCTTCACCT30.5253940455341506No Hit
ACCTGGGAAGAATTTAGAGATGAGTTG30.5253940455341506No Hit
TCGGACATTTGGTCGAGCACTCGCT30.5253940455341506No Hit
GAATTTAGAGATGAGTTGATGGGATCCAC30.5253940455341506No Hit
ACCGCAGGTTCGGACATTTGGTCGA30.5253940455341506No Hit
ACATGACAGCTCTGGCTAAGGAGGTTCT30.5253940455341506No Hit
CCGCAGGTTCGGACATTTGGTCGAGCA30.5253940455341506No Hit
TTCGGACATTTGGTCGAGCACTCGCA30.5253940455341506No Hit
AAGAGAGAGTGGCAAAGAAAGATTG30.5253940455341506No Hit
TCCGCGGGATTACGCCTGAACGCCTCTA30.5253940455341506No Hit
TTTGGTCGAGCACTCG30.5253940455341506No Hit
AAATAAAATCATGACCAAAGCAGACGCCT30.5253940455341506No Hit
CGCTACCATCCGCGGGATTACGCCTGAAC30.5253940455341506No Hit
CCCAAAGAGACTTGCTGGTATGCGAACAAA20.3502626970227671No Hit
CACATTTTTGGGACCATTTAAGA20.3502626970227671No Hit
CAGAAGACATTTCCCCATTGAAGAC20.3502626970227671No Hit
GCCTGAACGCCTCTAAG20.3502626970227671No Hit
CTAAGGCCGTATCCGTTCTAATCGT20.3502626970227671No Hit
CGCCTGAACGCCTCTAAGGCCGTATCCGT20.3502626970227671No Hit
GCAAAGAAAGATTGAAAACAATAATGAC20.3502626970227671No Hit
CGCGGGATTACGCCTGAACGCCTCTAA20.3502626970227671No Hit
CCGCGGGATTACGCCTGAACGCCTCTAAGG20.3502626970227671No Hit
GACGCTACCATCCGCGGGATTACG20.3502626970227671No Hit
CGAAATGGCTGCCTTC20.3502626970227671No Hit
CCGCGGGATTACGCCTGAACGCCT20.3502626970227671No Hit
GGGATTACGCCTGAACGCCTCTAAGGC20.3502626970227671No Hit
CATTTGGTCGAGCACTC20.3502626970227671No Hit
TTTGGGACCATTTAAGAAGATACATAA20.3502626970227671No Hit
TAAAATTATCGACGAAATGGC20.3502626970227671No Hit
AAGGAGGTTCTGAAGATCCTGAGAAGAG20.3502626970227671No Hit
CAGTTGTAGAAGAAGATGCCAGAGC20.3502626970227671No Hit
GCGGGATTACGCCTGAACGCCTC20.3502626970227671No Hit
TTGGTCGAGCACTCGCT20.3502626970227671No Hit
CCTGAGAAGAGAGAGTGGCAAAGAA20.3502626970227671No Hit
ATTTGGTCGAGCACTCG20.3502626970227671No Hit
GCCTGAACGCCTCTA20.3502626970227671No Hit
CCGCGGGATTACGCCTGAACGCCTCTAAG20.3502626970227671No Hit
TTATCGACGAAATGGCTGCCTTCAC20.3502626970227671No Hit
CTGAGAAGAGAGAGTG20.3502626970227671No Hit
ACATTTGGTCGAGCACT20.3502626970227671No Hit
CCTCAGCCAAGATGTCCACAG20.3502626970227671No Hit
AAGGCCGTATCCGTTCTAATCGT20.3502626970227671No Hit
CGGACATTTGGTCGAGCACTCGC20.3502626970227671No Hit
GGGATTACGCCTGAACGCCTCTAAG20.3502626970227671No Hit
TGCCTGGTGTTCCCC20.3502626970227671No Hit
ATTACGCCTGAACGCCTCTAA20.3502626970227671No Hit
GCAGGTTCGGACATTTGGTCGAGCACTC20.3502626970227671No Hit
CTGGTATGCGAACAAAC20.3502626970227671No Hit
ACGCCTGAACGCCTCT20.3502626970227671No Hit
TCTAAGGCCGTATCCGTTCTAATC20.3502626970227671No Hit
GGTCGAGCACTCGCTCGAGCGGGC20.3502626970227671No Hit
ACGCCTGAACGCCTC20.3502626970227671No Hit
CGGGATTACGCCTGAACGCCTCTAAGGC20.3502626970227671No Hit
CGCCTCCTCAGCCAAGATGTCCACA20.3502626970227671No Hit
TTACGCCTGAACGCCTCTA20.3502626970227671No Hit
ATGACCAAAGCAGAC20.3502626970227671No Hit
GATTACGCCTGAACGCCTCTAAG20.3502626970227671No Hit
ATCCGCGGGATTACGCCTGAACGCCT20.3502626970227671No Hit
CAGTTGTAGAAGAAGATGCCAGAGCAGC20.3502626970227671No Hit
CCTGGGAAGAATTTAG10.17513134851138354No Hit
CCGCAGGTTCGGACATTTG10.17513134851138354No Hit
AATGGCTGCCTTCAC10.17513134851138354No Hit
CTCGCTCGAGCGGGCGGTGGTGCGAC10.17513134851138354No Hit
ACCATCCGCGGGATTACCCCTGAACGCCT10.17513134851138354No Hit
ACCGCAGGTTCGGACATTTGGTCGAG10.17513134851138354No Hit
AATAAAATCATGACCAAAGC10.17513134851138354No Hit
TTTGGTCGAGCACTC10.17513134851138354No Hit
CGAGTTGGGGGTGGT10.17513134851138354No Hit
CGCAGGTTCGGACATTTGGTCGA10.17513134851138354No Hit
ATTACGCCTGAACGCCTCTA10.17513134851138354No Hit
CTGGATGCCTATAAG10.17513134851138354No Hit
GGATTACGCCTGAACGCCTCT10.17513134851138354No Hit
AACGCCTCTAAGGCCGTATCCGGTCTAATC10.17513134851138354No Hit
ATCCGTTCTAATCGTAGTGG10.17513134851138354No Hit
GAATTTAGAGATGATTTGATGGGATCCAC10.17513134851138354No Hit
AAGGCCGTATCCGTTCTAAT10.17513134851138354No Hit
TGAAGAAACGGAATGGCCAAC10.17513134851138354No Hit
CCGCGGGATTACGCCTG10.17513134851138354No Hit
AAGGCCGTATCCGTTCTAATCGTAG10.17513134851138354No Hit
ATTTGGTCGAGCACTC10.17513134851138354No Hit
GTTGATGGGATCCAC10.17513134851138354No Hit
CTGAACGCCTCTAAGGCCGTATCCGTTCTA10.17513134851138354No Hit
CGCTACCATCCGCGGGA10.17513134851138354No Hit
GCAGGTTCGGACATTTGGTCGAGCACTCG10.17513134851138354No Hit
AATTATCGACGAAATGGCTGCCTTCA10.17513134851138354No Hit
AAGGCCGTATCCGTTCTA10.17513134851138354No Hit
CCGCGGGATTACGCCTGAACGCC10.17513134851138354No Hit
CGCCAGTTGTAGAAGAAGATGCC10.17513134851138354No Hit
CGCCTGAACGCCTCTAAGGCCGTATCC10.17513134851138354No Hit
GAGAGAGGAGGCAAT10.17513134851138354No Hit
ACGCCTGAACGCCTCTA10.17513134851138354No Hit
ACTAATCTATAGACCAACTCCTAATATTA10.17513134851138354No Hit
ATGGGATCCACGGAT10.17513134851138354No Hit
CGTATCCGTTCTAATCGTAGT10.17513134851138354No Hit
TGGGACCATTTAAGAAG10.17513134851138354No Hit
AGAGGAACCGCAGGTTCGGACATTTGGTCG10.17513134851138354No Hit
TTACGCCTGAACGCCTCT10.17513134851138354No Hit
ACGCCTCCTCAGCCAAGATGTCCACA10.17513134851138354No Hit
ACCGCAGGTTCGGACATTTGGT10.17513134851138354No Hit
GTAGGTACAGCAAAGGGTGGAAAGAGTATC10.17513134851138354No Hit
ATGGCTGCCTTCACC10.17513134851138354No Hit
ACATTTGGTCGAGCA10.17513134851138354No Hit
ATCCGCGGGATTACGCCTGAACGCCTCTAA10.17513134851138354No Hit
CTCTAAGGCCGTATC10.17513134851138354No Hit
TCTGAAGATCCTGAGAAGAGAGAGTGGCAA10.17513134851138354No Hit
GATTACGCCTGAACGCCTCTAAGGCC10.17513134851138354No Hit
GCCTGAACGCCTCTAAGGCCGTATCCGT10.17513134851138354No Hit
GGCCGTATCCGTTCTAA10.17513134851138354No Hit
GTGGCAAAGAAAGATTG10.17513134851138354No Hit
GGGATTACGCCTGAACGCCT10.17513134851138354No Hit
TGCTGCGCTTACTATCAAA10.17513134851138354No Hit
ACACACACACACACACA10.17513134851138354No Hit
TCTGAAGATCCTGAGAAG10.17513134851138354No Hit
CGGACATTTGGTCGAGCACTCGCA10.17513134851138354No Hit
ATCGACGAAATGGCTGCCTTCAC10.17513134851138354No Hit
GCCTCTAAGGCCGTATCCGTTCTAAT10.17513134851138354No Hit
ATTACGCCTGAACGCCTCT10.17513134851138354No Hit
CGGACATTTGGTCGAGCACTCGCT10.17513134851138354No Hit
GGCCGTATCCGTTCTAATCGTAGTGGT10.17513134851138354No Hit
TCGAGCGGGCGGTGGTGCGACGCTACCA10.17513134851138354No Hit
TGAAATGGCTGCCTTC10.17513134851138354No Hit
TGGTCGAGCACTCGCT10.17513134851138354No Hit
CCGCAGGTTCGGACATTTGGTCGAGC10.17513134851138354No Hit
GGGATTACGCCTGAACGCCTCTAA10.17513134851138354No Hit
TCCGCGGGATTACGCCTGAACGCCT10.17513134851138354No Hit
GTGCGACGCTACCATCCGCGGGATTAC10.17513134851138354No Hit
TGGCCGCTGCAGCTCTGCTGG10.17513134851138354No Hit
AATTATCGACGAAATGGCTGCCTTCACCTG10.17513134851138354No Hit
AAGGCCGTATCCGTACTAATCG10.17513134851138354No Hit
TGTTCCCCAAAGAGAC10.17513134851138354No Hit
GTTCGGACATTTGGTCGAGCACTCGAA10.17513134851138354No Hit
TATCGACGAAATGGCTGCCTTCACCTGGGA10.17513134851138354No Hit
TTATCGACGAAATGGCTGCCTTC10.17513134851138354No Hit
TCTGGCTCGTTGGTGCCTGGTGTTCCCCAA10.17513134851138354No Hit
TCAAATCCAGACTTCG10.17513134851138354No Hit
CGACGCTACCATCCGCGGGATTACGCCTG10.17513134851138354No Hit
TGCGACGCTACCATCC10.17513134851138354No Hit
CGCCTGAACGCCTCTAACGCCGTATCCGT10.17513134851138354No Hit
GTATCACTCTGGCTC10.17513134851138354No Hit
AATAATGACTGGAATC10.17513134851138354No Hit
TTCGGACATTTGGTCGAGCACTCGAA10.17513134851138354No Hit
TCCGCGGGATTACGCCTGAACGCCTCTAAG10.17513134851138354No Hit
TAGGTACAGCAAAGGGTGGAAAGAGTATCA10.17513134851138354No Hit
CACCTGGGAAGAATTTAG10.17513134851138354No Hit
AGGTTCGGACAGTTGGTCGAGCACTCGC10.17513134851138354No Hit
GCCTGAACGCCTCTAAGGCCGTATCCG10.17513134851138354No Hit
GGCAAAGAAAGATTGAAAACAATAATGAC10.17513134851138354No Hit
CTGAAGATCCTGAGAAGAGAGAGTGGCAA10.17513134851138354No Hit
CCTGAGAAGAGAGAGTGGCAAAGAAA10.17513134851138354No Hit
ATTTGGTCGAGCACTCGCT10.17513134851138354No Hit
ATCGACGAAATGGCTGCCTTCACCTG10.17513134851138354No Hit
CTCGAGCGGGCGGTG10.17513134851138354No Hit
ACGCCTCCTCAGCCAAGATGTCCAC10.17513134851138354No Hit
CTGAAGATCCTGAGAA10.17513134851138354No Hit
CATTTGGTCGAGCACTCGCT10.17513134851138354No Hit
GAAGATCCTGAGAAGAG10.17513134851138354No Hit
TTGGTCGAGCACTCG10.17513134851138354No Hit
CTGAAGATCCTGAGAAGAGAGAGTGGCAAA10.17513134851138354No Hit
CTGAACGCCTCTAAGGCCGT10.17513134851138354No Hit
GACGCTACCATCCGCGGGATTACGCCTGA10.17513134851138354No Hit
CTCAAAGGCCGTATCCGTTCTAATC10.17513134851138354No Hit
CGTATCCGTTCTAATCGTA10.17513134851138354No Hit
TAGAGATGAGTTGATGGGATCCAC10.17513134851138354No Hit
ACCATCCGCGGGATTACGCCTGAACGC10.17513134851138354No Hit
CTAAGGCCGTATCCGTTCTAATCGTAG10.17513134851138354No Hit
TGCGACGCTACCATCCG10.17513134851138354No Hit
TGCTGGTATGCGAACAAACAAAGAAGACA10.17513134851138354No Hit
CTCTAAGGCCGTATCC10.17513134851138354No Hit
TCCTCAGCCAAGATGTCCACAG10.17513134851138354No Hit
CCCAAAGAGACTTGC10.17513134851138354No Hit
CCTCAGCCAAGATGTCCAC10.17513134851138354No Hit
GAGGAAATAAAATCATGACCAAAGCAC10.17513134851138354No Hit
TGAAGATCCTGAGAAGAG10.17513134851138354No Hit
CAAAGAAAGATTGAAAACAATAATGACTGG10.17513134851138354No Hit
CGACGAAATGGCTGCCTTCACCTG10.17513134851138354No Hit
AGGATTTATTGTTTAAGTT10.17513134851138354No Hit
GCACTCGCTCGAGCGG10.17513134851138354No Hit
GAGGTTCTGAAGATCCTGAGAAGAGAG10.17513134851138354No Hit
AAAGAGACTTGCTGGTATGCGAA10.17513134851138354No Hit
CGCCTCTAAGGCCGTATCCGTTCTA10.17513134851138354No Hit
ACACATTTTTGGGACCA10.17513134851138354No Hit
AGAGAGTGGCAAAGAAAGATTG10.17513134851138354No Hit
GTCGAGCACTCGCTCGAGCGGGC10.17513134851138354No Hit
CTCTGCTGGATGCCTATAAGGACTAATC10.17513134851138354No Hit
GAGAGTGGCAAAGAAAGATTG10.17513134851138354No Hit
CTCGAGCGGGCGGTGGTGC10.17513134851138354No Hit
ACACATTTTTGGGACCATTTAAGA10.17513134851138354No Hit
AGGTACAGCAAAGGGTGGAAAGAGTATC10.17513134851138354No Hit
AATTATCGACGAAATGGCTGCCTTCACCT10.17513134851138354No Hit
ATCGACGAAATGGCT10.17513134851138354No Hit
GGCCGTATCCGTTCTAATC10.17513134851138354No Hit
CCTGAACGCCTCTAAGG10.17513134851138354No Hit
TACGCCTGAACGCCTCTAA10.17513134851138354No Hit
TGAAGATCCTGAGAAGAGAGAGTGGCA10.17513134851138354No Hit
AATGAAAACCTTTAAGAACAA10.17513134851138354No Hit
ATCTAGAATAATAATCACAAACTAA10.17513134851138354No Hit
TGCTGGTATGCGAACAAACACAGAAGAC10.17513134851138354No Hit
AAAGATTGAAAACAATAATGAC10.17513134851138354No Hit
GGATTACGCCTGAACGCCTCTAAGGC10.17513134851138354No Hit
GATTACGCCTGAACGCCT10.17513134851138354No Hit
TGAGAAGAGAGAGTGGCAA10.17513134851138354No Hit
CGCGGGATTACGCCTGAACGCCTC10.17513134851138354No Hit
AGAGAGAGTGGCAAAGAAAGATTG10.17513134851138354No Hit
GCGAACAAACACAGAAGAC10.17513134851138354No Hit
AAGGCCGTATCCGTTCTAATCGTAGTG10.17513134851138354No Hit
ATTTGGTCGAGCACTCGCTC10.17513134851138354No Hit
CGCGGGATTACGCCTGAACGCCTCTA10.17513134851138354No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)