FastQCFastQC Report
Mon 28 Nov 2022
S1_Sudafrica.Phlebovirus_segment_S.15-35nt.new.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS1_Sudafrica.Phlebovirus_segment_S.15-35nt.new.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences453
Sequences flagged as poor quality0
Sequence length20-35
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCAGTCAAATCCGTGGATCCCATCAACTC163.532008830022075No Hit
TAATATGTATCTTCTTAAATGGTCCCAAAAATGT112.4282560706401766No Hit
TTCTTCTACAACTGGCGAACTTGATCCCAAGGAG81.7660044150110374No Hit
TACAACTGGCGAACTTGATCCCAAGGAGACAAATC81.7660044150110374No Hit
TCCGAAGTCTGGATTTGATAGTAAGCGCAGCATT81.7660044150110374No Hit
AATATGTATCTTCTTAAATGGTCCCAAAAATGTGT81.7660044150110374No Hit
CCATCTGTGGACATCTTGGCTGAGGAGGCGTC71.545253863134658No Hit
CAGGATCTTCAGAACCTCCTTAGCCAGAGCTGTC71.545253863134658No Hit
AGTCAAATCCGTGGATCCCATCAACTC71.545253863134658No Hit
ACAACTGGCGAACTTGATCCCAAGGAGACA71.545253863134658No Hit
TTTAACTTAATATGTATCTTCTTAAATGGTCCCA71.545253863134658No Hit
CTTTTCAGTCAAATCCGTGGATCCCATCAACTC71.545253863134658No Hit
AGTGATACTCTTTCCACCCTTTGCTGTACC71.545253863134658No Hit
TACAACTGGCGAACTTGATCCCAAGGAGACA71.545253863134658No Hit
CCTCCGAAGTCTGGATTTGATAGTAAGCGCAGC61.3245033112582782No Hit
GCTTTGTCTTCGACTTATAAGCCTGCCCTCCAATC61.3245033112582782No Hit
GTCAAATCCGTGGATCCCATCAACTCATCTCT61.3245033112582782No Hit
TTCCCAGGTGAAGGCAGCCATTTCGTCGATA61.3245033112582782No Hit
TCTGCTTTGGTCATGATTTTATTTCCTCTTTCCG61.3245033112582782No Hit
CTCCGAAGTCTGGATTTGATAGTAAGCGCAGC61.3245033112582782No Hit
CCCAGGTGAAGGCAGCCATTTCGTCGATAA61.3245033112582782No Hit
TCAGTCAAATCCGTGGATCCCATCAACTCATC61.3245033112582782No Hit
GAACTTGATCCCAAGGAGACAAATCTAATTGCC61.3245033112582782No Hit
CCCAGGTGAAGGCAGCCATTTCGTCGATA61.3245033112582782No Hit
AATCCGTGGATCCCATCAACTCATC61.3245033112582782No Hit
CTACAACTGGCGAACTTGATCCCAAGGAGACAAA51.1037527593818985No Hit
CCAGGTGAAGGCAGCCATTTCGTCGATAA51.1037527593818985No Hit
CCTTTTCAGTCAAATCCGTGGATCCCATCAACTC51.1037527593818985No Hit
TTTTAACTTAATATGTATCTCCTTAAATGGTCCC51.1037527593818985No Hit
TACAACTGGCGAACTTGATCCCAAGGAGACAAAT51.1037527593818985No Hit
CTACAACTGGCGAACTTGATCCCAAGGA51.1037527593818985No Hit
GGCAGCCATTTCGTCGATAATTTTAACTTAATATG51.1037527593818985No Hit
CCAGGTGAAGGCAGCCATTTCGTCGATAAT51.1037527593818985No Hit
TTCAGTCAAATCCGTGGATCCCATCAACTCA51.1037527593818985No Hit
TCAATCTTTCTTTGCCACTCTCTCTTCTCAG51.1037527593818985No Hit
TTTGTCTTCGACTTATAAGCCTGCCCTCCAATC51.1037527593818985No Hit
TTTGGGGAACACCAGGCACCAACGAGCCAGAGT40.8830022075055187No Hit
TTTGTCTTCGACTTATAAGCCTGCCCTC40.8830022075055187No Hit
TACAACTGGCGAACTTGATCCCAAGG40.8830022075055187No Hit
GTTGATAGTAGTTTCAATTATTTCTATTAGGAAC40.8830022075055187No Hit
TTTGTCTTCGACTTATAAGCCTGCCCTCCA40.8830022075055187No Hit
AACTCGACCAGAAATGCTGCTCT30.6622516556291391No Hit
TCTCTCTTCTCAGGATCTTCAGAACC30.6622516556291391No Hit
CTTTCCGAGAAGAGAATGATTCCAGTCA30.6622516556291391No Hit
CTGGCGAACTTGATCCCAAGGAGACAAATCTA30.6622516556291391No Hit
ACAACTGGCGAACTTGATCCCAAGGAGACAAA30.6622516556291391No Hit
AGAGTGGAAACCATCAGACATCTGGGTGTTGGCC30.6622516556291391No Hit
TCTGTGGACATCTTGGCTGAG30.6622516556291391No Hit
TTCTTCTACAACTGGCGAACTTGATCCCA30.6622516556291391No Hit
TTCTTAAATGGTCCCAAAAATGTG30.6622516556291391No Hit
TTTGTCTTCGACTTATAAGCCTGCCCTCC30.6622516556291391No Hit
TCAAATCCGTGGATCCCATCAACTCATCTC30.6622516556291391No Hit
TCGACTTATAAGCCTGCCCTCCC30.6622516556291391No Hit
CAGGTGAAGGCAGCCATTTCGTCGATA30.6622516556291391No Hit
TTCTACAACTGGCGAACTTGATC30.6622516556291391No Hit
ACACAACGCCCCTTCCACAACCACCCCC30.6622516556291391No Hit
GTCAAATCCGTGGATCCCATCAACTCATCTC30.6622516556291391No Hit
AACTGGCGAACTTGATCCCAAGGAGAC30.6622516556291391No Hit
TTCCTTTTCAGTCAAATCCGTGGATCCC20.44150110375275936No Hit
CAGAGTGATACTCTTTCCACCCTTTGCTG20.44150110375275936No Hit
CACAACCACCCCCAACTCGAC20.44150110375275936No Hit
GGAGGCGTCTGCTTTGGTCATGA20.44150110375275936No Hit
ACAACTGGCGAACTTGATCCC20.44150110375275936No Hit
CATCTGTGGACATCTTGGCTGAGGAGGCG20.44150110375275936No Hit
TCTACAACTGGCGAACTTGATCCC20.44150110375275936No Hit
AATCCGTGGATCCCATCAACTCATCTC20.44150110375275936No Hit
TGTGGACATCTTGGCTGAGGA20.44150110375275936No Hit
CTTCTACAACTGGCGAACTTGATCCC20.44150110375275936No Hit
GTGGACATCTTGGCTGAGGAG20.44150110375275936No Hit
TATCTTCTTAAATGGTCCCAAAA20.44150110375275936No Hit
ATTTCCTTTTCAGTCAAATCCGTGGATCCC20.44150110375275936No Hit
CCATCTGTGGACATCTTGGCTGAGGAGGAGTC20.44150110375275936No Hit
ACTCGACCAGAAATGCTGCTC20.44150110375275936No Hit
CAACTGGCGAACTTGATCCCAAGGAGACAAA20.44150110375275936No Hit
AACGCCCCTTCCACAACCAC20.44150110375275936No Hit
TCTTCTTCTACAACTGGCGAACTTGATCCC20.44150110375275936No Hit
CATCTGGGTGTTGGCCATTC20.44150110375275936No Hit
ACAACTGGCGAACTTGATCCCAAGG20.44150110375275936No Hit
CGTGGATCCCATCAACTCATCT20.44150110375275936No Hit
TAAGCCTGCCCTCCAATCACC20.44150110375275936No Hit
TTGGCAGGTATCAGCGCTGATAGAGTGGAA20.44150110375275936No Hit
CCCAGGTGAAGGCAGCCATTTCGTCGATAAAAAAA20.44150110375275936No Hit
CCGTGGATCCCATCAACTCATCTC20.44150110375275936No Hit
TTTCCGAGAAGAGAATGATTCCAGTC20.44150110375275936No Hit
CTACAACTGGCGAACTTGATCCCAAGG20.44150110375275936No Hit
CAACGCCCCTTCCACAACCACCC20.44150110375275936No Hit
AATATGTATCTTCTTAAATGGTCCCAAAAATGTGG20.44150110375275936No Hit
CAGTCAAATCCGTGGATCCCATCAACTC20.44150110375275936No Hit
TGTCTTCGACTTATAAGCCTGCCCTCC20.44150110375275936No Hit
TCGACTTATAAGCCTGCCCTCCAATCACC20.44150110375275936No Hit
TTCAGTCAAATCCGTGGATCCCATCAA20.44150110375275936No Hit
TGAAGGCAGCCATTTCGTCGATAATTTT20.44150110375275936No Hit
TTTCAGTCAAATCCGTGGATCCCATCAACT20.44150110375275936No Hit
CTTTTCAGTCAAATCCGTGGATCCCATCAAC10.22075055187637968No Hit
TTTCCGAGACGAGAATGATTCCAGTC10.22075055187637968No Hit
CTCTGGCATCTTCTTCTACAACTG10.22075055187637968No Hit
GGCGTAATCCCGCGGATGGTAGC10.22075055187637968No Hit
ATAATTAAGTTGATAGTAGTTTCAATTAT10.22075055187637968No Hit
CATCTCTAAATTCTTCCCAGGAGAAGGCAG10.22075055187637968No Hit
TAGTAGTTTCAATTATTTATATTAGGAAC10.22075055187637968No Hit
ACAACGCCCCTTCCACAACCACCC10.22075055187637968No Hit
AGCTTTGTCTTCGACTTATAAGCC10.22075055187637968No Hit
CAGTCAAATCCGTGGATCCCATCAACTCA10.22075055187637968No Hit
CAACTGGCGAACTTGATCCCAAGGAGACAA10.22075055187637968No Hit
TTCAGTCAAATCCGTGGATCCC10.22075055187637968No Hit
CAGGCGTAATCCCGCGGCTGGTAGCGTCG10.22075055187637968No Hit
AAGTCTGGATTTGATAGTAAGC10.22075055187637968No Hit
CAGGTGAAGGCAGCCATTTCGTCGATAAT10.22075055187637968No Hit
GGCGTTCAGGCGTAATCCCGCGGAGG10.22075055187637968No Hit
ACCACCCCCAACTCGACCAGAAATGCTGCT10.22075055187637968No Hit
CGACTTATAAGCCTGCCCTCCC10.22075055187637968No Hit
TCCGTGGATCCCATCAACTCATCT10.22075055187637968No Hit
TNTAACTTAATATGTATCTTCTTAAATGGTCCCA10.22075055187637968No Hit
CTCTTCTCAGGATCTTCAGAA10.22075055187637968No Hit
GCCTTAGAGGCGTTCAGGCGTAAT10.22075055187637968No Hit
ACACAACGCCCCTTCCACAACCA10.22075055187637968No Hit
CTCTCTTCTCAGGATCTTCAGAACC10.22075055187637968No Hit
CATCTGTGGACATCTAGGCTGAGGAGGC10.22075055187637968No Hit
AGTGCTCGACCAAATGTCCGAACC10.22075055187637968No Hit
TNTTAACTTAATATGTATCTCCTTAAATGGTCCC10.22075055187637968No Hit
AATTGAAGCCATCTGTGGACATCTTGGCTGA10.22075055187637968No Hit
CAGCCATTTCGTCGATAATTTTAA10.22075055187637968No Hit
TCTTATTCTAGATGTTAGAGTACATAAA10.22075055187637968No Hit
GTAATCCCGCGGATGGTAGCGTCG10.22075055187637968No Hit
AGTGATACTCTTTCCACCCTTTG10.22075055187637968No Hit
ACTACGATTAGAACGGATACGGCCTTAGAGGCGTT10.22075055187637968No Hit
GAAGTCTGGATTTGATAGTAAGC10.22075055187637968No Hit
TACAACTGGCGAACTTGATCCCAAGGAGAC10.22075055187637968No Hit
CAAATCCGTGGATCCCATCAACTCATCT10.22075055187637968No Hit
ATAAGCCTGCCCTCCAATCACCTCCGA10.22075055187637968No Hit
CAAATCCGTGGATCCCATCAACTCATCTC10.22075055187637968No Hit
CACAACGCCCCTGCCACAACCACCCCC10.22075055187637968No Hit
GCTCGACCAAATGTCCGAACC10.22075055187637968No Hit
AGCGAGTGCTCGACCAAATGTCCGAAC10.22075055187637968No Hit
TTCTACAACTGGCGAACTTGATCCC10.22075055187637968No Hit
CGCTCGAGCGAGTGCTCGACCAAAT10.22075055187637968No Hit
AATCCGTGGATCCCATCAACTCA10.22075055187637968No Hit
ATGGTAGCGTCGCACCACCGCCCGCTCGAGCGAG10.22075055187637968No Hit
CTTATAAGCCTGCCCTCCAATCACCTCCGA10.22075055187637968No Hit
AGTCTGGATTTGATAGTAAGC10.22075055187637968No Hit
CTTAAATGGTCCCAAAAATGTGT10.22075055187637968No Hit
AACCTCCTTAGCCCGAGCTGTCATGTCAAA10.22075055187637968No Hit
CCTAGGGCTTAATACTAATGTTAG10.22075055187637968No Hit
TAGGCGTTCAGGCGTAATCCCGCGGATG10.22075055187637968No Hit
CTCCGAAGTCTGGATTTGATAGTAAGCGCAGCATT10.22075055187637968No Hit
ATCAGACATCTGGGTGTTGGCCAT10.22075055187637968No Hit
CCTAGGGCTTAATACTAATGTTAGTTTG10.22075055187637968No Hit
ATCAGGCGTAATCCCGCGGATGGTA10.22075055187637968No Hit
TTCAGAACCTCCTTAGCCAGAAA10.22075055187637968No Hit
TTGAAGCCATCTGTGGACATCTTGGCT10.22075055187637968No Hit
AGAAGAGAATGATTCCAGTCATTATAGTTTTCA10.22075055187637968No Hit
AAATCCGTGGATCCCATCAACT10.22075055187637968No Hit
AAATCCGGGATAATTAAGTTGATA10.22075055187637968No Hit
TCAAATCCGTGGATCCCATCAACTCATCTCT10.22075055187637968No Hit
GGCGAACTTGATCCCAAGGAGACAAATCT10.22075055187637968No Hit
CCAGGTGAAGGCAGCCATATCGTCGATAAT10.22075055187637968No Hit
CAGGCGTAATCCCGCGGATGGTAGCGT10.22075055187637968No Hit
GGCGTAATCCCGCGGATGGTAGCGTCGCACCA10.22075055187637968No Hit
CTGGCGAACTTGATCCCAAGG10.22075055187637968No Hit
TTTTCAGTCAAATCCGTGGATCCCATCAACTCATC10.22075055187637968No Hit
TACAACTGGCGAACTTGATCCCAAGGA10.22075055187637968No Hit
CTCTGGCATCTTCTTCTACAACT10.22075055187637968No Hit
CTCTAAATTCTTCCCAGGTGAAG10.22075055187637968No Hit
CCGTGGATCCCATCAACTCATC10.22075055187637968No Hit
ACAACTGGCGAACTTGATCCCAAGGA10.22075055187637968No Hit
CCACCCCCAACTCGACCAGAAATGCTGCT10.22075055187637968No Hit
CACATCTTTACACAACGCCCCTTCCA10.22075055187637968No Hit
AGAACGGATACGGCCTTAGAGG10.22075055187637968No Hit
CTACGATTAGAACGGATACGGCCAAA10.22075055187637968No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)