FastQCFastQC Report
Mon 28 Nov 2022
S1_Sudafrica.Sobemovirus_Segment_1.15-35nt.new.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS1_Sudafrica.Sobemovirus_Segment_1.15-35nt.new.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7650
Sequences flagged as poor quality0
Sequence length20-35
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTGTAGGCGACGACAATA4826.3006535947712425No Hit
TGAGTGTAGGCGACGACAATAC3774.928104575163399No Hit
ATTGAGTGTAGGCGACGACAATAC3364.392156862745098No Hit
TGAGTGTAGGCGACGACAATACC2272.9673202614379086No Hit
TGAATTGAGTGTAGGCGACGACAATACC2022.6405228758169934No Hit
ATTGAGTGTAGGCGACGACAATACCAGGAGTCCCG2002.6143790849673203No Hit
ATTGAGTGTAGGCGACGACAATACCAGGAGTCCC1972.5751633986928106No Hit
ACAGACTGAATTGAGTGTAGGCGACGACAATACC1952.549019607843137No Hit
ATTGAGTGTAGGCGACGACAATACC1752.287581699346405No Hit
ATTGAGTGTAGGCGACGACAATA1732.261437908496732No Hit
AGTGTAGGCGACGACAATACC1632.130718954248366No Hit
TTGAGTGTAGGCGACGACAATA1461.9084967320261437No Hit
TTGAGTGTAGGCGACGACAATAC1421.8562091503267975No Hit
CTGAATTGAGTGTAGGCGACGACAATACC1251.6339869281045754No Hit
ATTGAGTGTAGGCGACGACAATACCAGGAGTCC1191.5555555555555556No Hit
ACTGAATTGAGTGTAGGCGACGACAATACC1181.5424836601307188No Hit
CTGAATTGAGTGTAGGCGACGACAATAC1031.3464052287581698No Hit
TTGAGTGTAGGCGACGACAATACC1011.3202614379084967No Hit
AGTGTAGGCGACGACAATAC951.2418300653594772No Hit
AGACTGAATTGAGTGTAGGCGACGACAATACC901.1764705882352942No Hit
GACTGAATTGAGTGTAGGCGACGACAATACC901.1764705882352942No Hit
TGAATTGAGTGTAGGCGACGACAATAC851.1111111111111112No Hit
AATTGAGTGTAGGCGACGACAATACCAGGAGTCC811.0588235294117647No Hit
GAATTGAGTGTAGGCGACGACAATACC760.9934640522875817No Hit
TGAGTGTAGGCGACGACAATACCAGGAGTCCC730.9542483660130718No Hit
TGAGTGTAGGCGACGACAAT710.9281045751633987No Hit
TGAATTGAGTGTAGGCGACGACAATA710.9281045751633987No Hit
AATTGAGTGTAGGCGACGACAATACCAGGAGTCCC630.823529411764706No Hit
ACAGACTGAATTGAGTGTAGGCGACGACAATACCA630.823529411764706No Hit
TTGAGTGTAGGCGACGACAATACCAGGAGTCC570.7450980392156863No Hit
ACAGACTGAATTGAGTGTAGGCGACGACAATA560.7320261437908496No Hit
GAATTGAGTGTAGGCGACGACAATAC550.7189542483660131No Hit
AATTGAGTGTAGGCGACGACAATACC530.6928104575163399No Hit
ACAGACTGAATTGAGTGTAGGCGACGACA450.5882352941176471No Hit
TACAGACTGAATTGAGTGTAGGCGACGACAATA430.5620915032679739No Hit
TGAGTGTAGGCGACGACAATACCAGGAGTCC420.5490196078431373No Hit
GAGTGTAGGCGACGACAATA420.5490196078431373No Hit
TTGAGTGTAGGCGACGACAATACCAGGAGTCCC420.5490196078431373No Hit
TACAGACTGAATTGAGTGTAGGCGACGACAATACC410.5359477124183006No Hit
CTGAATTGAGTGTAGGCGACGACAATA410.5359477124183006No Hit
GACTGAATTGAGTGTAGGCGACGACAATAC380.49673202614379086No Hit
ATTGAGTGTAGGCGACGACAATACCAGGA360.4705882352941176No Hit
GACTGAATTGAGTGTAGGCGACGACAATA360.4705882352941176No Hit
AGACTGAATTGAGTGTAGGCGACGACAATA350.4575163398692811No Hit
ACTGAATTGAGTGTAGGCGACGACAATA350.4575163398692811No Hit
AGACTGAATTGAGTGTAGGCGACGACAATAC340.4444444444444444No Hit
ACTGAATTGAGTGTAGGCGACGACAATAC310.40522875816993464No Hit
GAGTGTAGGCGACGACAATAC290.3790849673202615No Hit
TACAGACTGAATTGAGTGTAGGCGACGACAATAC290.3790849673202615No Hit
TTGAGTGTAGGCGACGACAAT290.3790849673202615No Hit
TGAGTGTAGGCGACGACAATACCAGGAGT280.3660130718954248No Hit
GAATTGAGTGTAGGCGACGACAATA280.3660130718954248No Hit
TGAGTGTAGGCGACGACAATACCAGGAGTC280.3660130718954248No Hit
GTGTAGGCGACGACAATACC270.35294117647058826No Hit
GAATTGAGTGTAGGCGACGACAATACCAGGAGTCC270.35294117647058826No Hit
TTGAGTGTAGGCGACGACAATACCAGGAGTCCCG270.35294117647058826No Hit
TGAATTGAGTGTAGGCGACGACAATACCAGGAGTC260.33986928104575165No Hit
ACAGACTGAATTGAGTGTAGGCGACGACAATAC260.33986928104575165No Hit
ATTGAGTGTAGGCGACGACAATACCAGGAGTC260.33986928104575165No Hit
TGAGTGTAGGCGACGACAATAA250.32679738562091504No Hit
AATTGAGTGTAGGCGACGACAATAC250.32679738562091504No Hit
ATTGAGTGTAGGCGACGACAATACCAGGAGT250.32679738562091504No Hit
GAGTGTAGGCGACGACAATACC250.32679738562091504No Hit
ACTGAATTGAGTGTAGGCGACGACAATACCA240.3137254901960784No Hit
ATTGAGTGTAGGCGACGACAAT230.3006535947712418No Hit
AGACTGAATTGAGTGTAGGCGACGACAATACCA220.2875816993464052No Hit
AGTGTAGGCGACGACAATACCAGGAGT220.2875816993464052No Hit
TGAGTGTAGGCGACGACAATACCAGGAGTCCCGAG210.27450980392156865No Hit
ACTGAATTGAGTGTAGGCGACGACAATACCAGG200.261437908496732No Hit
TGAGTGTAGGCGACGACAATACCAGGAGTCCCG200.261437908496732No Hit
GAATTGAGTGTAGGCGACGACAATACCAGGA200.261437908496732No Hit
CAGACTGAATTGAGTGTAGGCGACGACAATACC200.261437908496732No Hit
TTGAGTGTAGGCGACGACAATACCAGGAGT190.24836601307189543No Hit
AATTGAGTGTAGGCGACGACAATA190.24836601307189543No Hit
GTGTACAGACTGAATTGAGTGTAGGCGACGACAAT190.24836601307189543No Hit
TTGAGTGTAGGCGACGACAATACCAGGAGTC190.24836601307189543No Hit
CGACAATACCAGGAGTCCCGAGCGCGCGT180.2352941176470588No Hit
ACAGACTGAATTGAGTGTAGGCGACGACAA170.2222222222222222No Hit
GACTGAATTGAGTGTAGGCGACGACAATACCAG170.2222222222222222No Hit
TGAATTGAGTGTAGGCGACGACAATACCAGGA170.2222222222222222No Hit
TACAGACTGAATTGAGTGTAGGCGACGACAAT170.2222222222222222No Hit
ATTGAGTGTAGGCGACGACAATAA160.20915032679738566No Hit
ACTGAATTGAGTGTAGGCGACGACAATACCAGGA160.20915032679738566No Hit
TGAGTGTAGGCGACGACAATACCAGGA160.20915032679738566No Hit
TTGAGTGTAGGCGACGACAATACCA160.20915032679738566No Hit
ACAGACTGAATTGAGTGTAGGCGACGA150.19607843137254902No Hit
ACTGAATTGAGTGTAGGCGACGACAATACCAGGAG140.1830065359477124No Hit
GAATTGAGTGTAGGCGACGACAATACCAGGAG140.1830065359477124No Hit
GACTGAATTGAGTGTAGGCGACGACAATACCAGGA140.1830065359477124No Hit
GCGCAATTGGAATATGTTGACTCGGAAGCAGCA140.1830065359477124No Hit
ACAGACTGAATTGAGTGTAGGCGACGACAAT130.16993464052287582No Hit
ACTGAATTGAGTGTAGGCGACGACAAT130.16993464052287582No Hit
ATTGAGTGTAGGCGACGACAATACA120.1568627450980392No Hit
GAATTGAGTGTAGGCGACGACAATACCAGGAGTC120.1568627450980392No Hit
GACTGAATTGAGTGTAGGCGACGACAATACCA120.1568627450980392No Hit
AATTGAGTGTAGGCGACGACAATACCAGG120.1568627450980392No Hit
TGAGTGTAGGCGACGACAATACA120.1568627450980392No Hit
CCTGAACGCCTCTAAGGCCGTATCCGTTCTAAT110.1437908496732026No Hit
AGTGTAGGCGACGACAATACCAGGAGTC110.1437908496732026No Hit
ATTGAGTGTAGGCGACGACAATACCAGG110.1437908496732026No Hit
AGTGTAGGCGACGACAATACCAGGA110.1437908496732026No Hit
TTGAGTGTAGGCGACGACAATACCAGGAGTCCCGA110.1437908496732026No Hit
TGAATTGAGTGTAGGCGACGACAATACCAGGAGT110.1437908496732026No Hit
TACAGACTGAATTGAGTGTAGGCGACGACAA110.1437908496732026No Hit
CGTATCCGTTCTAATCGTAGTGGTGTACAGACTGA100.130718954248366No Hit
CGGCCTGGGATTGGACGGCACC100.130718954248366No Hit
TGAATTGAGTGTAGGCGACGACAAT100.130718954248366No Hit
GCGACGACACCCTGATTCACCCTAGACAT100.130718954248366No Hit
CTGAATTGAGTGTAGGCGACGACAATACCAGGAGT100.130718954248366No Hit
CGGCCTGGGATTGGACGGCACCTTA100.130718954248366No Hit
GCCTGAACGCCTCTAAGGCCGTATCCGTTCTA100.130718954248366No Hit
TGAGTGTAGGCGACGACAATAAAA100.130718954248366No Hit
AATTGAGTGTAGGCGACGACAAT100.130718954248366No Hit
ATGCGCAATTGGAATATGTTGACTCGGAAGCAGCA90.1176470588235294No Hit
CGGCCTGGGATTGGACGGCACCTTACT90.1176470588235294No Hit
TACGCCTGAACGCCTCTAAGGCCGTATCCGTTCTA90.1176470588235294No Hit
GTACAGACTGAATTGAGTGTAGGCGACGACA90.1176470588235294No Hit
TTGAGTGTAGGCGACGACAATAAA90.1176470588235294No Hit
TACAGACTGAATTGAGTGTAGGCGACGACA90.1176470588235294No Hit
ATTGAGTGTAGGCGACGACAATACCA90.1176470588235294No Hit
ATGGGTGGATACAGCGGCTGCGGATACT90.1176470588235294No Hit
AGACTGAATTGAGTGTAGGCGACGACAATACCAG90.1176470588235294No Hit
ACTGAATTGAGTGTAGGCGACGACAATACCAG90.1176470588235294No Hit
GTGTACAGACTGAATTGAGTGTAGGCGACGACAA90.1176470588235294No Hit
GAATTGAGTGTAGGCGACGACA90.1176470588235294No Hit
GACTGAATTGAGTGTAGGCGACGACAAT80.10457516339869283No Hit
ATTGAGTGTAGGCGACGACAATAAAA80.10457516339869283No Hit
GCGCAATTGGAATATGTTGACTCGGAAGCAGC80.10457516339869283No Hit
TGCAGTCGTCTCCTGGTTTGCCTTATC80.10457516339869283No Hit
TGAATTGAGTGTAGGCGACGACAATACCAGG80.10457516339869283No Hit
AATTGAGTGTAGGCGACGACAATACCAGGAGT80.10457516339869283No Hit
GAATTGAGTGTAGGCGACGACAATACCAG80.10457516339869283No Hit
TGTACAGACTGAATTGAGTGTAGGCGACGACA80.10457516339869283No Hit
AAGGCCGTATCCGTTCTAATCGTAGTGGTGTACA80.10457516339869283No Hit
GAGACGACACCCTGATTCACCCT80.10457516339869283No Hit
GTATGTTGTATGATGACCCGGCTCTGTTTGGC80.10457516339869283No Hit
ATCGTAGTGGTGTACAGACTGAATTGAGTGTA80.10457516339869283No Hit
AGTGTAGGCGACGACAATACCAGGAGTCCCGAGC80.10457516339869283No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)