FastQCFastQC Report
Mon 28 Nov 2022
S1_Sudafrica.Sobemovirus_Segment_1.15-35nt.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS1_Sudafrica.Sobemovirus_Segment_1.15-35nt.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5707
Sequences flagged as poor quality0
Sequence length15-30
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTGTAGGCGACGACAATA4838.463290695636937No Hit
TGAGTGTAGGCGACGACAATAC3816.676011915191869No Hit
ATTGAGTGTAGGCGACGACAATAC3405.957595934816892No Hit
TGAGTGTAGGCGACGACAATACC2334.082705449448047No Hit
TGAATTGAGTGTAGGCGACGACAATACC2043.5745575608901348No Hit
AGTGTAGGCGACGACAATA1793.136499036271246No Hit
ATTGAGTGTAGGCGACGACAATACC1763.083932013316979No Hit
ATTGAGTGTAGGCGACGACAATA1743.048887331347468No Hit
AGTGTAGGCGACGACAATACC1682.9437532854389348No Hit
TTGAGTGTAGGCGACGACAATA1482.5933064657438236No Hit
TTGAGTGTAGGCGACGACAATAC1452.5407394427895564No Hit
CTGAATTGAGTGTAGGCGACGACAATACC1312.2954266690029788No Hit
ACTGAATTGAGTGTAGGCGACGACAATACC1222.1377256001401785No Hit
CTGAATTGAGTGTAGGCGACGACAATAC1061.8573681443840897No Hit
TTGAGTGTAGGCGACGACAATACC1031.8048011214298232No Hit
AGTGTAGGCGACGACAATAC981.717189416506045No Hit
TGAATTGAGTGTAGGCGACGACAATAC861.5069213246889785No Hit
GAATTGAGTGTAGGCGACGACAATACC781.366742596810934No Hit
TGAATTGAGTGTAGGCGACGACAATA731.2791308918871562No Hit
TGAGTGTAGGCGACGACAAT711.244086209917645No Hit
GAATTGAGTGTAGGCGACGACAATAC581.0162957771158225No Hit
AATTGAGTGTAGGCGACGACAATACC530.9286840721920449No Hit
ACAGACTGAATTGAGTGTAGGCGACGACA450.7885053443140003No Hit
CTGAATTGAGTGTAGGCGACGACAATA420.7359383213597337No Hit
GAGTGTAGGCGACGACAATA420.7359383213597337No Hit
GACTGAATTGAGTGTAGGCGACGACAATAC400.7008936393902225No Hit
ATTGAGTGTAGGCGACGACAATACCAGGA360.6308042754512003No Hit
AGACTGAATTGAGTGTAGGCGACGACAATA360.6308042754512003No Hit
ACTGAATTGAGTGTAGGCGACGACAATA360.6308042754512003No Hit
GACTGAATTGAGTGTAGGCGACGACAATA360.6308042754512003No Hit
AGTGTAGGCGACGACAAT350.6132819344664447No Hit
ACTGAATTGAGTGTAGGCGACGACAATAC330.5782372524969336No Hit
GAATTGAGTGTAGGCGACGACAATA320.5607149115121781No Hit
GTGTAGGCGACGACAATACC300.5256702295426668No Hit
GAGTGTAGGCGACGACAATAC290.5081478885579113No Hit
TTGAGTGTAGGCGACGACAAT290.5081478885579113No Hit
TGAGTGTAGGCGACGACAATACCAGGAGT280.49062554757315574No Hit
TGAGTGTAGGCGACGACAATACCAGGAGTC280.49062554757315574No Hit
AATTGAGTGTAGGCGACGACAATAC270.47310320658840016No Hit
GAGTGTAGGCGACGACAATACC260.45558086560364464No Hit
TGAGTGTAGGCGACGACAATAA250.43805852461888906No Hit
ATTGAGTGTAGGCGACGACAAT230.40301384264937795No Hit
AGTGTAGGCGACGACAATACCAGGAGT220.38549150166462237No Hit
AATTGAGTGTAGGCGACGACAATA200.35044681969511127No Hit
TTGAGTGTAGGCGACGACAATACCAGGAGT190.3329244787103557No Hit
CGCCTGAACGCCTCTAA180.31540213772560016No Hit
CGACAATACCAGGAGTCCCGAGCGCGCGT180.31540213772560016No Hit
ACAGACTGAATTGAGTGTAGGCGACGACAA170.2978797967408446No Hit
TTGAGTGTAGGCGACGACAATACCA170.2978797967408446No Hit
ATTGAGTGTAGGCGACGACAATAA160.28035745575608906No Hit
TGAGTGTAGGCGACGACAATACCAGGA160.28035745575608906No Hit
ACAGACTGAATTGAGTGTAGGCGACGA150.2628351147713334No Hit
GTGTAGGCGACGACAATA140.24531277378657787No Hit
AATTGAGTGTAGGCGACGACAATACCAGG140.24531277378657787No Hit
ACTGAATTGAGTGTAGGCGACGACAAT130.22779043280182232No Hit
ATTGAGTGTAGGCGACGACAATACA120.21026809181706677No Hit
TGAGTGTAGGCGACGACAATACA120.21026809181706677No Hit
AGTGTAGGCGACGACAATACCAGGAGTC110.19274575083231119No Hit
ATTGAGTGTAGGCGACGACAATACCAGG110.19274575083231119No Hit
AGTGTAGGCGACGACAATACCAGGA110.19274575083231119No Hit
CGGCCTGGGATTGGACGGCACC100.17522340984755563No Hit
TGAATTGAGTGTAGGCGACGACAAT100.17522340984755563No Hit
GCGACGACACCCTGATTCACCCTAGACAT100.17522340984755563No Hit
CGGCCTGGGATTGGACGGCACCTTA100.17522340984755563No Hit
TGAGTGTAGGCGACGACAATAAAA100.17522340984755563No Hit
AATTGAGTGTAGGCGACGACAAT100.17522340984755563No Hit
CGGCCTGGGATTGGACGGCACCTTACT90.15770106886280008No Hit
TTGAGTGTAGGCGACGACAATAAA90.15770106886280008No Hit
TACAGACTGAATTGAGTGTAGGCGACGACA90.15770106886280008No Hit
ATTGAGTGTAGGCGACGACAATACCA90.15770106886280008No Hit
ATGGGTGGATACAGCGGCTGCGGATACT90.15770106886280008No Hit
GAGTGTAGGCGACGACAAT90.15770106886280008No Hit
ATTGAGTGTAGGCGACGA90.15770106886280008No Hit
GAATTGAGTGTAGGCGACGACA90.15770106886280008No Hit
CGCCTGAACGCCTCTAAG80.14017872787804453No Hit
GACTGAATTGAGTGTAGGCGACGACAAT80.14017872787804453No Hit
ATTGAGTGTAGGCGACGACAATAAAA80.14017872787804453No Hit
TGAGTGTAGGCGACGACAA80.14017872787804453No Hit
TGCAGTCGTCTCCTGGTTTGCCTTATC80.14017872787804453No Hit
CGGCCTGGGATTGGAC80.14017872787804453No Hit
CGCCTGAACGCCTCTA80.14017872787804453No Hit
GAATTGAGTGTAGGCGACGACAATACCAG80.14017872787804453No Hit
GAGACGACACCCTGATTCACCCT80.14017872787804453No Hit
GCCTGAACGCCTCTAA80.14017872787804453No Hit
TGAGTGTAGGCGACGACAATAAA80.14017872787804453No Hit
GACTGAATTGAGTGTAGGCGACGACA70.12265638689328894No Hit
CGGCCTGGGATTGGACGGCACCTTAC70.12265638689328894No Hit
CTGAATTGAGTGTAGGCGACGA70.12265638689328894No Hit
CTGAATTGAGTGTAGGCGACGACAATACCA70.12265638689328894No Hit
AATTGAGTGTAGGCGACGACAATACCA70.12265638689328894No Hit
TTGAGTGTAGGCGACGACAATACCAGGA70.12265638689328894No Hit
TGAGTGTAGGCGACGACAATACCAGG60.10513404590853338No Hit
CGACGACACCCTGATTCACCCT60.10513404590853338No Hit
CCTCTAAGGCCGTATCCGTTCTAATCGT60.10513404590853338No Hit
TACAGCGGCTGCGGATA60.10513404590853338No Hit
AGCGCGCGTGGCCCTC60.10513404590853338No Hit
AGACTGAATTGAGTGTAGGCGACGACA60.10513404590853338No Hit
CGACGACACCCTGATTCACCC60.10513404590853338No Hit
CGCAACGGATATGGTCATCATTAAACT60.10513404590853338No Hit
GTGTAGGCGACGACAATAC60.10513404590853338No Hit
AGACGACACCCTGATTCACCC60.10513404590853338No Hit
CAATTGGAATATGTTGACTCGGAAGCAGC60.10513404590853338No Hit
AGTGTAGGCGACGACAATACCAGGAG60.10513404590853338No Hit
GCAATTGGAATATGTTGACTCGGAAGCAGC60.10513404590853338No Hit
AGTGTAGGCGACGACAATACCA60.10513404590853338No Hit
CACAGACTGAATTGAGTGTAGGCGACGAC60.10513404590853338No Hit
AGACTGAATTGAGTGTAGGCGACGACAAT60.10513404590853338No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)