FastQCFastQC Report
Mon 28 Nov 2022
S1_Sudafrica.Sobemovirus_segmet_2.15-35nt.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS1_Sudafrica.Sobemovirus_segmet_2.15-35nt.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9884
Sequences flagged as poor quality0
Sequence length15-30
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAAGAGAGTCGTTGGCACAGCATACA2902.934034803723189No Hit
AGAGAGTCGTTGGCACAGCA2892.92391744233104No Hit
AGAGAGTCGTTGGCACAGCATACA2722.7519222986645087No Hit
CAAGAGAGTCGTTGGCACAGCATACA2392.4180493727235937No Hit
TTCAAGAGAGTCGTTGGCACAGCA2352.377579927154998No Hit
CAAGAGAGTCGTTGGCACAGCA2232.256171590449211No Hit
TTTCAAGAGAGTCGTTGGCACAGCA2192.215702144880615No Hit
AAGAGAGTCGTTGGCACAGCATACA2132.1549979765277216No Hit
GAGAGTCGTTGGCACA2082.1044111695669767No Hit
TTTCAAGAGAGTCGTTGGCACAGCATACA1992.0133549170376366No Hit
GAGAGTCGTTGGCACAGC1971.9931201942533388No Hit
AGAGAGTCGTTGGCACA1921.9425333872925938No Hit
GAGAGTCGTTGGCACAGCA1881.9020639417239984No Hit
CAAGAGAGTCGTTGGCACAGC1861.8818292189397006No Hit
AAGAGAGTCGTTGGCACAGCA1701.7199514366653175No Hit
TTCAAGAGAGTCGTTGGCACAGC1691.7098340752731687No Hit
AAGAGAGTCGTTGGCACA1581.5985430999595305No Hit
CAAGAGAGTCGTTGGCACA1541.5580736543909348No Hit
CCTTTCAAGAGAGTCGTTGGCACAGCA1491.5074868474301901No Hit
TTCAAGAGAGTCGTTGGCACA1441.4569000404694454No Hit
AGAGAGTCGTTGGCACAGC1441.4569000404694454No Hit
TCAAGAGAGTCGTTGGCACAGCATACA1361.3759611493322543No Hit
GAGAGTCGTTGGCACAGCATACA1271.2849048968029138No Hit
CTTTCAAGAGAGTCGTTGGCACAGCA1251.264670174018616No Hit
AGAGAGTCGTTGGCACAGCATACAA1231.244435451234318No Hit
AGAGAGTCGTTGGCACAGCATA1181.1938486442735736No Hit
AAGAGAGTCGTTGGCACAGC1181.1938486442735736No Hit
CTTTCAAGAGAGTCGTTGGCACAGCATACA1161.1736139214892756No Hit
TCAAGAGAGTCGTTGGCACAGCA1101.112909753136382No Hit
CCTTTCAAGAGAGTCGTTGGCACAGC1071.0825576689599354No Hit
TTTCAAGAGAGTCGTTGGCACAGC1061.0724403075677864No Hit
TTTCAAGAGAGTCGTTGGCACAGCATA1021.0319708619991905No Hit
CAAGAGAGTCGTTGGCACAGCATA1011.0218535006070417No Hit
CTTTCAAGAGAGTCGTTGGCACAGC991.0016187778227439No Hit
TTCAAGAGAGTCGTTGGCACAGCATACAA930.9409146094698503No Hit
TTTCAAGAGAGTCGTTGGCACA900.9105625252934034No Hit
TTCAAGAGAGTCGTTGGCACAGCATA840.84985835694051No Hit
TCAAGAGAGTCGTTGGCACAGC780.7891541885876164No Hit
AAGAGAGTCGTTGGCACAGCATACAA730.7385673816268717No Hit
TCAAGAGAGTCGTTGGCACA670.6778632132739781No Hit
CCTTTCAAGAGAGTCGTTGGCACA650.6576284904896803No Hit
GAGAGTCGTTGGCAC630.6373937677053824No Hit
CAAGAGAGTCGTTGGCACAGCATACAA610.6171590449210845No Hit
AAGAGAGTCGTTGGCACAGCATA580.5868069607446378No Hit
CTTTCAAGAGAGTCGTTGGCACA570.5766895993524889No Hit
TTCAAGAGAGTCGTTGGCACAGCATAAA540.546337515176042No Hit
TCAAGAGAGTCGTTGGCACAGCATA530.5362201537838932No Hit
TTCAAGAGAGTCGTTGGCACAGCATACAAA530.5362201537838932No Hit
AAGAGAGTCGTTGGCACAGCATAAA510.5159854309995953No Hit
TCAAGAGAGTCGTTGGCACAGCATACAA490.49575070821529743No Hit
TTCAAGAGAGTCGTTGGCACAGCATAC480.48563334682314846No Hit
AGAAGATTGTATTCCGCGGCGC420.424929178470255No Hit
CAAGAGAGTCGTTGGCAC410.41481181707810605No Hit
CAAGAGAGTCGTTGGCACAGCATAAA410.41481181707810605No Hit
AGAGAGTCGTTGGCAC410.41481181707810605No Hit
AGAGAGTCGTTGGCACAGCATAAA400.4046944556859571No Hit
CAAGAGAGTCGTTGGCACAGCATAA390.3945770942938082No Hit
TTCAAGAGAGTCGTTGGCAC380.38445973290165925No Hit
CCTTTCAAGAGAGTCGTTGGCAC350.3541076487252125No Hit
AAGAGAGTCGTTGGCAC330.3338729259409146No Hit
AGAGAGTCGTTGGCACAGCATAA330.3338729259409146No Hit
TTCAAGAGAGTCGTTGGCACAGCATACAC330.3338729259409146No Hit
TTTCAAGAGAGTCGTTGGCACAGCATAC320.3237555645487657No Hit
AAGGCCTTTCAAGAGAGTCGTTGGCACAGC310.3136382031566168No Hit
TTCAAGAGAGTCGTTGGCACAGCATACACA310.3136382031566168No Hit
AGAGAGTCGTTGGCACAGCATTCA310.3136382031566168No Hit
TTTCAAGAGAGTCGTTGGCACAGCATACAA300.3035208417644678No Hit
AAGAGAGTCGTTGGCACAGCATAA280.28328611898017No Hit
TTTTCAAGAGAGTCGTTGGCACAGC270.273168757588021No Hit
TTTCAAGAGAGTCGTTGGCAC270.273168757588021No Hit
GAGAGTCGTTGGCACAG270.273168757588021No Hit
GGCCTTTCAAGAGAGTCGTTGGCACAGCA270.273168757588021No Hit
AGAGAGTCGTTGGCACAGCATAC270.273168757588021No Hit
TTCAAGAGAGTCGTTGGCACAGCATAA260.2630513961958721No Hit
TCAAGAGAGTCGTTGGCACAGCATAAA260.2630513961958721No Hit
CAAGAGAGTCGTTGGCACAGCATAC260.2630513961958721No Hit
TTCAAGAGAGTCGTTGGC260.2630513961958721No Hit
CTTTTCAAGAGAGTCGTTGGCACAGC250.2529340348037232No Hit
GCCTTTCAAGAGAGTCGTTGGCACAGCA250.2529340348037232No Hit
TTTCAAGAGAGTCGTTGGCACAGCATACGC240.24281667341157423No Hit
AAGAGAGTCGTTGGC230.23269931201942534No Hit
TTTCAAGAGAGTCGTTGGCACAGCATAAA230.23269931201942534No Hit
CAAGAGAGTCGTTGGC230.23269931201942534No Hit
TCAAGAGAGTCGTTGGCAC220.22258195062727643No Hit
AGAGAGTCGTTGGCACAGCATACGC220.22258195062727643No Hit
GGCCTTTCAAGAGAGTCGTTGGCACAGC220.22258195062727643No Hit
CCTTTCAAGAGAGTCGTTGGCACAG220.22258195062727643No Hit
GAGAGTCGTTGGCACAGCATTCA210.2124645892351275No Hit
TTTCAAGAGAGTCGTTGGC210.2124645892351275No Hit
CTTTTCAAGAGAGTCGTTGGCACA210.2124645892351275No Hit
CTTTCAAGAGAGTCGTTGGCAC200.20234722784297854No Hit
GAGAGTCGTTGGCACAGCATAC200.20234722784297854No Hit
TTTCAAGAGAGTCGTTGGCACAGCATAA180.18211250505868068No Hit
AAGAGAGTCGTTGGCACAGCATAC180.18211250505868068No Hit
GCCTTTCAAGAGAGTCGTTGGCACAGC180.18211250505868068No Hit
GAGAGTCGTTGGCACAGCATAA180.18211250505868068No Hit
CAAGAGAGTCGTTGGCACAGCATTCA180.18211250505868068No Hit
AAGAGAGTCGTTGGCACAGCAAACA170.1719951436665318No Hit
AGAGAGTCGTTGGCA170.1719951436665318No Hit
AGAGAGTCGTTGGCACAGCATACAC160.16187778227438285No Hit
GAGAGTCGTTGGCACAGCATGC160.16187778227438285No Hit
CTTTTCAAGAGAGTCGTTGGCACAG150.1517604208822339No Hit
AAGAAGATTGTATTCCGCGGCGC150.1517604208822339No Hit
TTCAAGAGAGTCGTTGGCACAGCATGC150.1517604208822339No Hit
CCTTTCAAGAGAGTCGTTGGC150.1517604208822339No Hit
TCAAGAGAGTCGTTGGCACAGCATTCA150.1517604208822339No Hit
TCAAGAGAGTCGTTGGCACAGCATAC150.1517604208822339No Hit
AAGAGAGTCGTTGGCACAGCATGC140.141643059490085No Hit
TTCAAGAGAGTCGTTGGCACAGCAAACA140.141643059490085No Hit
TTCAAGAGAGTCGTTGGCACAGCAT140.141643059490085No Hit
TTCAAGAGAGTCGTTGGCACAGCATACGC140.141643059490085No Hit
CTTTTCAAGAGAGTCGTTGGCACAGCA140.141643059490085No Hit
TTTCAAGAGAGTCGTTGGCACAG140.141643059490085No Hit
CAAGAGAGTCGTTGGCA140.141643059490085No Hit
AGAGGAAGAATGGGCCCGCGA130.13152569809793604No Hit
CAAGAGAGTCGTTGGCACAGCATACAT130.13152569809793604No Hit
CAAGAGAGTCGTTGGCACAGCATACAC130.13152569809793604No Hit
GGCCTTTCAAGAGAGTCGTTGGCACA130.13152569809793604No Hit
TTTCAAGAGAGTCGTTGGCACAGCAAACA130.13152569809793604No Hit
CAAGAGAGTCGTTGGCACAG120.12140833670578711No Hit
TTTTCAAGAGAGTCGTTGGCACAGCA120.12140833670578711No Hit
GAGTCGTTGGCACAGCA120.12140833670578711No Hit
TCAAGAGAGTCGTTGGCACAG120.12140833670578711No Hit
TTCAAGAGAGTCGTTGGCACAG120.12140833670578711No Hit
AGAGAGTCGTTGGCACAG120.12140833670578711No Hit
AGGCCTTTCAAGAGAGTCGTTGGCACAGCA120.12140833670578711No Hit
TTTCAAGAGAGTCGTTGGCACAGCAT120.12140833670578711No Hit
TTTCAAGAGAGTCGTTGGCA110.11129097531363821No Hit
GAGAGTCGTTGGCACAGCAT110.11129097531363821No Hit
AAGAGAGTCGTTGGCACAGCATTCA110.11129097531363821No Hit
TTCAAGAGAGTCGTTGGCACAGCATACGCC110.11129097531363821No Hit
TCAAGAGAGTCGTTGGCACAGCATAA110.11129097531363821No Hit
GCCTTTCAAGAGAGTCGTTGGCACA100.10117361392148927No Hit
AGAGAGTCGTTGGCACAGCATATCA100.10117361392148927No Hit
GAGAGTCGTTGGCACAGCATAAA100.10117361392148927No Hit
AAGAGAGTCGTTGGCACAGCATACGC100.10117361392148927No Hit
GCCTTTCAAGAGAGTCGTTGGCAC100.10117361392148927No Hit
CAAGAGAGTCGTTGGCACAGCATGC100.10117361392148927No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)