FastQCFastQC Report
Mon 28 Nov 2022
S1_Sudafrica.Sobemovirus_segmet_2.15-35nt.new.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS1_Sudafrica.Sobemovirus_segmet_2.15-35nt.new.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8832
Sequences flagged as poor quality0
Sequence length20-35
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAAGAGAGTCGTTGGCACAGCATACA2893.2721920289855073No Hit
AGAGAGTCGTTGGCACAGCA2873.2495471014492754No Hit
AGAGAGTCGTTGGCACAGCATACA2703.057065217391304No Hit
CAAGAGAGTCGTTGGCACAGCATACA2382.6947463768115942No Hit
TTCAAGAGAGTCGTTGGCACAGCA2322.6268115942028984No Hit
CAAGAGAGTCGTTGGCACAGCA2202.4909420289855073No Hit
TTTCAAGAGAGTCGTTGGCACAGCA2182.4682971014492754No Hit
AAGAGAGTCGTTGGCACAGCATACA2122.4003623188405796No Hit
TTTCAAGAGAGTCGTTGGCACAGCATACA1982.2418478260869565No Hit
CAAGAGAGTCGTTGGCACAGC1842.083333333333333No Hit
AAGAGAGTCGTTGGCACAGCA1691.9134963768115945No Hit
TTCAAGAGAGTCGTTGGCACAGC1691.9134963768115945No Hit
CCTTTCAAGAGAGTCGTTGGCACAGCA1481.6757246376811596No Hit
TTCAAGAGAGTCGTTGGCACA1441.6304347826086956No Hit
CCTTTCAAGAGAGTCGTTGGCACAGCATACA1391.573822463768116No Hit
TCAAGAGAGTCGTTGGCACAGCATACA1361.5398550724637683No Hit
GAGAGTCGTTGGCACAGCATACA1261.4266304347826086No Hit
CTTTCAAGAGAGTCGTTGGCACAGCA1251.4153079710144927No Hit
AGAGAGTCGTTGGCACAGCATACAA1211.370018115942029No Hit
AAGAGAGTCGTTGGCACAGC1181.3360507246376812No Hit
AGAGAGTCGTTGGCACAGCATA1161.3134057971014492No Hit
CTTTCAAGAGAGTCGTTGGCACAGCATACA1151.3020833333333335No Hit
TCAAGAGAGTCGTTGGCACAGCA1091.2341485507246377No Hit
CCTTTCAAGAGAGTCGTTGGCACAGC1071.2115036231884058No Hit
TTTCAAGAGAGTCGTTGGCACAGC1041.177536231884058No Hit
TTTCAAGAGAGTCGTTGGCACAGCATA1021.154891304347826No Hit
CAAGAGAGTCGTTGGCACAGCATA991.1209239130434783No Hit
CTTTCAAGAGAGTCGTTGGCACAGC971.0982789855072463No Hit
TTCAAGAGAGTCGTTGGCACAGCATACAA921.0416666666666665No Hit
TTTCAAGAGAGTCGTTGGCACA901.0190217391304348No Hit
TTCAAGAGAGTCGTTGGCACAGCATA840.9510869565217392No Hit
TCAAGAGAGTCGTTGGCACAGC760.8605072463768116No Hit
AAGAGAGTCGTTGGCACAGCATACAA730.8265398550724637No Hit
TCAAGAGAGTCGTTGGCACA660.7472826086956522No Hit
CCTTTCAAGAGAGTCGTTGGCACA640.7246376811594203No Hit
CAAGAGAGTCGTTGGCACAGCATACAA610.6906702898550725No Hit
AAGAGAGTCGTTGGCACAGCATA580.6567028985507246No Hit
CTTTCAAGAGAGTCGTTGGCACA570.6453804347826086No Hit
TTCAAGAGAGTCGTTGGCACAGCATACAAA530.6000905797101449No Hit
TTCAAGAGAGTCGTTGGCACAGCATAAA530.6000905797101449No Hit
TCAAGAGAGTCGTTGGCACAGCATA520.588768115942029No Hit
AAGAGAGTCGTTGGCACAGCATAAA510.577445652173913No Hit
TCAAGAGAGTCGTTGGCACAGCATACAA490.5548007246376812No Hit
TTCAAGAGAGTCGTTGGCACAGCATAC480.5434782608695652No Hit
AAGGCCTTTCAAGAGAGTCGTTGGCACAGCA430.4868659420289855No Hit
CAAGAGAGTCGTTGGCACAGCATAAA410.4642210144927536No Hit
AGAAGATTGTATTCCGCGGCGC400.4528985507246377No Hit
AGAGAGTCGTTGGCACAGCATAAA400.4528985507246377No Hit
CAAGAGAGTCGTTGGCACAGCATAA390.44157608695652173No Hit
TTCAAGAGAGTCGTTGGCAC370.4189311594202899No Hit
CCTTTCAAGAGAGTCGTTGGCAC350.396286231884058No Hit
CCTTTCAAGAGAGTCGTTGGCACAGCATAAA350.396286231884058No Hit
TTTCAAGAGAGTCGTTGGCACAGCATACAAA340.3849637681159421No Hit
AGAGAGTCGTTGGCACAGCATAA330.3736413043478261No Hit
TTTCAAGAGAGTCGTTGGCACAGCATAC320.36231884057971014No Hit
TTCAAGAGAGTCGTTGGCACAGCATACAC320.36231884057971014No Hit
AAGGCCTTTCAAGAGAGTCGTTGGCACAGC310.3509963768115942No Hit
AGAGAGTCGTTGGCACAGCATTCA310.3509963768115942No Hit
TTCAAGAGAGTCGTTGGCACAGCATACACA300.33967391304347827No Hit
ATGAATCCGAGGGGGGGCCATATTAGGGTCGAAC300.33967391304347827No Hit
TTTCAAGAGAGTCGTTGGCACAGCATACAA290.3283514492753623No Hit
AAGAGAGTCGTTGGCACAGCATAA280.3170289855072464No Hit
TTTTCAAGAGAGTCGTTGGCACAGC270.30570652173913043No Hit
TTTCAAGAGAGTCGTTGGCAC270.30570652173913043No Hit
AGAGAGTCGTTGGCACAGCATAC270.30570652173913043No Hit
TTCAAGAGAGTCGTTGGCACAGCATAA260.2943840579710145No Hit
GGCCTTTCAAGAGAGTCGTTGGCACAGCA260.2943840579710145No Hit
TCAAGAGAGTCGTTGGCACAGCATAAA250.28306159420289856No Hit
CAAGAGAGTCGTTGGCACAGCATAC250.28306159420289856No Hit
CTTTTCAAGAGAGTCGTTGGCACAGC250.28306159420289856No Hit
GCCTTTCAAGAGAGTCGTTGGCACAGCA250.28306159420289856No Hit
TTTCAAGAGAGTCGTTGGCACAGCATACGC230.26041666666666663No Hit
TTTCAAGAGAGTCGTTGGCACAGCATAAA230.26041666666666663No Hit
TTCCTATGGTTGCCGAGGCCTACCCCTTTGTCTG220.2490942028985507No Hit
GGCCTTTCAAGAGAGTCGTTGGCACAGC220.2490942028985507No Hit
CCTTTCAAGAGAGTCGTTGGCACAG220.2490942028985507No Hit
TTTCAAGAGAGTCGTTGGCACAGCATACACA210.2377717391304348No Hit
GAGAGTCGTTGGCACAGCATTCA210.2377717391304348No Hit
CTTTTCAAGAGAGTCGTTGGCACA210.2377717391304348No Hit
AGAGAGTCGTTGGCACAGCATACGC210.2377717391304348No Hit
CTTTCAAGAGAGTCGTTGGCAC200.22644927536231885No Hit
GAAGGCCTTTCAAGAGAGTCGTTGGCACAGCA190.2151268115942029No Hit
CTTTCAAGAGAGTCGTTGGCACAGCATACAA190.2151268115942029No Hit
GAGAGTCGTTGGCACAGCATAC190.2151268115942029No Hit
TTTCAAGAGAGTCGTTGGCACAGCATAA180.20380434782608695No Hit
AAGAGAGTCGTTGGCACAGCATAC180.20380434782608695No Hit
GCCTTTCAAGAGAGTCGTTGGCACAGC180.20380434782608695No Hit
GAGAGTCGTTGGCACAGCATAA180.20380434782608695No Hit
CAAGAGAGTCGTTGGCACAGCATTCA180.20380434782608695No Hit
AAGAGAGTCGTTGGCACAGCAAACA170.19248188405797104No Hit
AGAGAGTCGTTGGCACAGCATACAC160.18115942028985507No Hit
GAGAGTCGTTGGCACAGCATGC160.18115942028985507No Hit
CTTTTCAAGAGAGTCGTTGGCACAG150.16983695652173914No Hit
GATGAATCCGAGGGGGGGCCATATTAGGGTCGAAC150.16983695652173914No Hit
AAGAAGATTGTATTCCGCGGCGC150.16983695652173914No Hit
TTCAAGAGAGTCGTTGGCACAGCATGC150.16983695652173914No Hit
CCTTTCAAGAGAGTCGTTGGC150.16983695652173914No Hit
TCAAGAGAGTCGTTGGCACAGCATTCA150.16983695652173914No Hit
ATCGATTGGTTAGGGAGTTGACCTTGGTGCAGCA150.16983695652173914No Hit
TCAAGAGAGTCGTTGGCACAGCATAC150.16983695652173914No Hit
AAGAGAGTCGTTGGCACAGCATGC140.1585144927536232No Hit
TTCAAGAGAGTCGTTGGCACAGCAAACA140.1585144927536232No Hit
TTCAAGAGAGTCGTTGGCACAGCAT140.1585144927536232No Hit
TTCAAGAGAGTCGTTGGCACAGCATACGC140.1585144927536232No Hit
AGAGGAAGAATGGGCCCGCGA130.14719202898550726No Hit
CAAGAGAGTCGTTGGCACAGCATACAT130.14719202898550726No Hit
CAAGAGAGTCGTTGGCACAGCATACAC130.14719202898550726No Hit
GGCCTTTCAAGAGAGTCGTTGGCACA130.14719202898550726No Hit
TTTCAAGAGAGTCGTTGGCACAGCAAACA130.14719202898550726No Hit
CTTTTCAAGAGAGTCGTTGGCACAGCA130.14719202898550726No Hit
TTTCAAGAGAGTCGTTGGCACAG130.14719202898550726No Hit
GATGAATCCGAGGGGGGGCCATATTAGGGTCGA120.1358695652173913No Hit
ATGAATCCGAGGGGGGGCCATATTAGGGTCGAA120.1358695652173913No Hit
TTTTCAAGAGAGTCGTTGGCACAGCA120.1358695652173913No Hit
TCAAGAGAGTCGTTGGCACAG120.1358695652173913No Hit
TTCAAGAGAGTCGTTGGCACAG120.1358695652173913No Hit
AGGCCTTTCAAGAGAGTCGTTGGCACAGCA120.1358695652173913No Hit
CTTTCAAGAGAGTCGTTGGCACAGCATACGC120.1358695652173913No Hit
TTTCAAGAGAGTCGTTGGCACAGCAT120.1358695652173913No Hit
TTTCAAGAGAGTCGTTGGCA110.12454710144927535No Hit
CAAGAGAGTCGTTGGCACAG110.12454710144927535No Hit
CGATTGGTTAGGGAGTTGACCTTGGTGCAGCAGGA110.12454710144927535No Hit
GAGAGTCGTTGGCACAGCAT110.12454710144927535No Hit
AAGAGAGTCGTTGGCACAGCATTCA110.12454710144927535No Hit
TTACGGGTGCGATCGTCTGGGAGGGGGAAAC110.12454710144927535No Hit
TCAAGAGAGTCGTTGGCACAGCATAA110.12454710144927535No Hit
CTTTTCAAGAGAGTCGTTGGCACAGCATTCA110.12454710144927535No Hit
AAGGCCTTTCAAGAGAGTCGTTGGCACAGCAT110.12454710144927535No Hit
GCCTTTCAAGAGAGTCGTTGGCACA100.11322463768115942No Hit
TTTACGATATTTGTTTAACTGGGGCACCTTC100.11322463768115942No Hit
TTGACCTTGGTGCAGCAGGAGTTTATAGAGGA100.11322463768115942No Hit
AGAGAGTCGTTGGCACAGCATATCA100.11322463768115942No Hit
ATTTCTACTACAAGCCTGCAGTGGGTACCACCTT100.11322463768115942No Hit
GAGAGTCGTTGGCACAGCATAAA100.11322463768115942No Hit
AAGAGAGTCGTTGGCACAGCATACGC100.11322463768115942No Hit
GCCTTTCAAGAGAGTCGTTGGCAC100.11322463768115942No Hit
CAATGGTCTCGTGTTTGACCCTCTGCCTGCT100.11322463768115942No Hit
GAAGGCCTTTCAAGAGAGTCGTTGGCACAGC100.11322463768115942No Hit
TTCAAGAGAGTCGTTGGCACAGCATACGCC100.11322463768115942No Hit
CAAGAGAGTCGTTGGCACAGCATGC100.11322463768115942No Hit
GATGAATCCGAGGGGGGGCCATATTAGGGTCGAA90.10190217391304347No Hit
GATGAATCCGAGGGGGGGCCATATTAGGGTCGAC90.10190217391304347No Hit
TATCGATTGGTTAGGGAGTTGACCTTGGTGCAGCA90.10190217391304347No Hit
CTTTCAAGAGAGTCGTTGGCACAGCATAAA90.10190217391304347No Hit
TTTCAAGAGAGTCGTTGGCACAGCATGC90.10190217391304347No Hit
TTTCAAGAGAGTCGTTGGCACAGCATACATT90.10190217391304347No Hit
CTTACGGGTGCGATCGTCTGGGAGGGGGAAAAG90.10190217391304347No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)