FastQCFastQC Report
Mon 28 Nov 2022
S1_Sudafrica.Sobemovirus_segmet_2.15-35nt.new.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS1_Sudafrica.Sobemovirus_segmet_2.15-35nt.new.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46
Sequences flagged as poor quality0
Sequence length20-23
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGAAATCGTGAACTCAGAA36.521739130434782No Hit
AGAGCCCATGAATCTGATCACCA24.3478260869565215No Hit
CGAAAAGAGACCGTAAAAGAGC24.3478260869565215No Hit
TCTTCCTCTTGCGCGCCTTCC24.3478260869565215No Hit
GAAGACATCGCCCTCCACTCC24.3478260869565215No Hit
CATTCTTCCTCTTGCGCGCCT24.3478260869565215No Hit
ACCACTGGCGCGTCTGCAACC24.3478260869565215No Hit
GAGCCCATGAATCTGATCACCA24.3478260869565215No Hit
GAAAAGAGACCGTAAAAGAGC24.3478260869565215No Hit
CCATTCTTCCTCTTGCGCGCC24.3478260869565215No Hit
CAGGCAGAGGGTCAAACACGA24.3478260869565215No Hit
AGAGCCCATGAATCTGATCACC12.1739130434782608No Hit
AATATGGCCCCCCCTCGGATT12.1739130434782608No Hit
CTCCCAGACGATCGCACCCG12.1739130434782608No Hit
ATACCACTGGCGCGTCTGCAA12.1739130434782608No Hit
GCGTCTGCAACCTCTCCCTACCA12.1739130434782608No Hit
GCAGAGGGTCAAACACGAGAC12.1739130434782608No Hit
TCTCAACCACCGCGTATCCAC12.1739130434782608No Hit
CTATGAACTCCTGCTGCACCA12.1739130434782608No Hit
GAGCCCATGAATCTGATCACACG12.1739130434782608No Hit
AGAGCCCATGAATCTGATCACA12.1739130434782608No Hit
CGGCGTTTCCCCCTCCCAGACC12.1739130434782608No Hit
AAGAGACCGTAAAAGAGCCCA12.1739130434782608No Hit
ATCGCGGGCCCATTCTTCCTC12.1739130434782608No Hit
CGGCGTTTCCCCCTCCCAGAC12.1739130434782608No Hit
TCGAAATCCCAGTCAACACCG12.1739130434782608No Hit
GAGCCCATGAATCTGATCACC12.1739130434782608No Hit
CCATTCTTCCTCTTGCGCGCCA12.1739130434782608No Hit
AAGACATCGCCCTCCACTCC12.1739130434782608No Hit
CTTGGAGGGCCCCGGCAGCGC12.1739130434782608No Hit
ATGGCCCCCCCTCGGATTCAT12.1739130434782608No Hit
GTTTGTCCAAAAGCAAAAGGGG12.1739130434782608No Hit
CCGAAGAGGAGAAGACATCGCC12.1739130434782608No Hit
ATCGCAAGGTTCCCCGGGCT12.1739130434782608No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)