FastQCFastQC Report
Mon 28 Nov 2022
S1_Sudafrica.Tombus-like_2_monopartite.15-35nt.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS1_Sudafrica.Tombus-like_2_monopartite.15-35nt.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106
Sequences flagged as poor quality0
Sequence length15-30
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCGATGTGACTGGCCCGG1917.92452830188679No Hit
TCTCGATGTGACTGGCCCGGC65.660377358490567No Hit
CTTGTATTTGGTGGCACTCATTT43.7735849056603774No Hit
TTTTGGCTGCTTGCAGGAGAA43.7735849056603774No Hit
TGGTCCGTAGTCTCCCTGAGC43.7735849056603774No Hit
TATCCGAAATTGTATGACTAGTT32.8301886792452833No Hit
CGACTGTGACGTGGGCATCGAA21.8867924528301887No Hit
CTCGATGTGACTGGCCCGGCG21.8867924528301887No Hit
TTGCGGGTAGGTAACA21.8867924528301887No Hit
ATTTTATAGGTCATGC21.8867924528301887No Hit
CTTGCAGGAGAAGAATGGTGCC21.8867924528301887No Hit
TCTTCAAACTGACGGCCCATG21.8867924528301887No Hit
TATCTGAGAGCCTCGACTGTGACGTAAA21.8867924528301887No Hit
GCATCCGCATGTTTTTTGGCT21.8867924528301887No Hit
TCCGAAATTGTATGACTAGTT21.8867924528301887No Hit
GTCCTTTGTGTAGGTGTGGTGTTCCAAT21.8867924528301887No Hit
TCTCGCGACCCATGC21.8867924528301887No Hit
GAAATATCTGAGAGCCTCGACA21.8867924528301887No Hit
ATTCTCGCGACCCATGC21.8867924528301887No Hit
TCAAACTGACGGCCCATGGTG21.8867924528301887No Hit
GCCTGCTATCGCTAGACA10.9433962264150944No Hit
CATCCGCATGTTTTTTGGCT10.9433962264150944No Hit
CAATTTCTTTGTGTAGCGGCCA10.9433962264150944No Hit
GGTGATGTCTCCTGAGTTGAC10.9433962264150944No Hit
TTTCTCGATGTGACTGGCCCG10.9433962264150944No Hit
CCGTCGTTGTCGAGTCGTACCA10.9433962264150944No Hit
ACACAGAGTCCAGGATGTAGC10.9433962264150944No Hit
TCACTATTCCTGTCCTTTG10.9433962264150944No Hit
TTCTCGCGACCCATGC10.9433962264150944No Hit
TTCTCGATGTGACTGGCCCG10.9433962264150944No Hit
GTATGTGGTCCGTAGTCTCCC10.9433962264150944No Hit
TCCGTAGTCTCCCTGAG10.9433962264150944No Hit
CGGCCTAGCTCCATGTTAAAG10.9433962264150944No Hit
AGGCAATAGAATTTTGGGTGGGA10.9433962264150944No Hit
TCCAAGCCATCCTGCCCGTACC10.9433962264150944No Hit
CAGCCTCCTGAAGATGCACTC10.9433962264150944No Hit
TAGTGTATGGAGTGTCTATTC10.9433962264150944No Hit
GATCTCATATTTGTTGGTGTA10.9433962264150944No Hit
TTCAAACTGACGGCCCA10.9433962264150944No Hit
ATTCACTATTCCTGTCCTTTG10.9433962264150944No Hit
TATCCTATTCTGTTCCGGGCG10.9433962264150944No Hit
CAATATCCGAAATTGTATGACTAGTT10.9433962264150944No Hit
CGACTTCATGTATGTCCTCACCT10.9433962264150944No Hit
CGATCTCAGTCCAGTATCCGA10.9433962264150944No Hit
CAAGGACAGACATTGGCATGCAA10.9433962264150944No Hit
TCGTGTAAGTTTGGGATGAGCA10.9433962264150944No Hit
CCATCACTACAGAGTCGTCCC10.9433962264150944No Hit
AGTCTCCTCGAGCGCGTCCAC10.9433962264150944No Hit
ATATCCGAAATTGTATGACTA10.9433962264150944No Hit
CAAGGACAGACATTGGCATGC10.9433962264150944No Hit
GTCCTGGACTGCGTCGAAAA10.9433962264150944No Hit
TCCTGGACTGCGTCGAAAAA10.9433962264150944No Hit
CTGTGGAAATATCTGAGAGCC10.9433962264150944No Hit
AGGCAATAGAATTTTGGGTGG10.9433962264150944No Hit
CTTCTAGGATTGTGGGCTTGTGTCGTCCAA10.9433962264150944No Hit
GATGTGACTGGCCCGGCGTTG10.9433962264150944No Hit
GGAAAACACAGAGTCCAGGACCA10.9433962264150944No Hit
CGATGTGACTGGCCCGGCG10.9433962264150944No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)