FastQCFastQC Report
Fri 14 Oct 2022
S1.Phlebovirus_segment_M.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS1.Phlebovirus_segment_M.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3559
Sequences flagged as poor quality0
Sequence length12-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAAGTGATTGTTATGGGAGACATGACCACCAAATATAAGGACCCTGAA1113.1188536105647655No Hit
GTGATTGTTATGGGAGACATGACCACCAAATATAAGGACCCTGAAATGAA661.8544534981736445No Hit
AAGAAATGTAATGAGACATCGAACGTCTACTGCTCCACCAAGAAGACAGC551.5453779151447036No Hit
TTCCATTGACTCAACCTGCCTTAAAGTGTTAAAACAAGAGACAGTCACCA521.4610845743186287No Hit
AGTGATCAAGGAGAACTGTGCCAACTGTGATGTCAAGTGTGTGAATGGAG521.4610845743186287No Hit
GAAAATTGCAGATACGGAGATTCAGGAACAGTCACTTGCACAACTTCCGA511.4329867940432706No Hit
AGTGCAAGATTGCGTGATTGAGGGAGTCTCAGACTGCAATGGAGATGAGT511.4329867940432706No Hit
GATACGGAGATTCAGGAACAGTCACTTGCACAACTTCCGAAACAACGAGA491.376791233492554No Hit
ACCAAATATAAGGACCCTGAAATGAAAGATGAGACATTCGAGTGCAAGAG491.376791233492554No Hit
AGACAAAGTGATTGTTATGGGAGACATGACCACCAAATATAAGGACCCTG471.3205956729418376No Hit
TAGAAGACAAAGTGATTGTTATGGGAGACATGACCACCAAATATAAGGAC461.2924978926664794No Hit
TCGGCTCTGGAGAGGCTGCACCTGCAAGAAGACATTCAGCAGAATGGGTT461.2924978926664794No Hit
ATCGGTCTATGAGGAGAAGAAAGTGGAGTGCAATGCCATCAGTGTCTGCA441.236302332115763No Hit
TACTAGTTCTGTGCATTGTATCCATAATGATCAATTCCGCAGATTCTTGC441.236302332115763No Hit
ATGGACTGAATGTTCCCAAGTAGAAGGAGGGGTTACCAACGGCTGTGCTG431.2082045518404048No Hit
CAGGGCTAATGTGAAAGGAGAGATCATCACGGCTAAGACAAAGAAAGAGG431.2082045518404048No Hit
GAAAGTGGAGTGCAATGCCATCAGTGTCTGCAACATGATAACTTGCACCT421.1801067715650464No Hit
TGTTATGGGAGACATGACCACCAAATATAAGGACCCTGAAATGAAAGATG421.1801067715650464No Hit
GAAGAAAGCTTGTGTGAATGGAAAAAAGGAGGTTGAGAATGTGCAATGGG411.152008991289688No Hit
CTCAAACTAACAATATTATCAGTGGATCTGGTCTGTAAGCCATCTGCCAT411.152008991289688No Hit
ACAAAGTGCAAGATTGCGTGATTGAGGGAGTCTCAGACTGCAATGGAGAT411.152008991289688No Hit
TGTTTACTTAAAGAGCTCATGCCTGTGGGTTAGGAAAGGAATTTCCAATC401.12391121101433No Hit
AAGTGCAAGATTGCGTGATTGAAGGAGTCTCAGACTGCAATGGAGATGAG391.0958134307389715No Hit
ATGATCAATTCCGCAGATTCTTGCACTAACACTCAGAATCTAAAGGGAAA391.0958134307389715No Hit
CTTTCGTGTTGTGATTAGAACAACTCATGGTGAGTTCACACCTCAGGCAT391.0958134307389715No Hit
AGTGATTGTTATGGGAGACATGACCACCAAATATAAGGACCCTGAAATGA391.0958134307389715No Hit
GATTGTTATGGGAGACATGACCACCAAATATAAGGACCCTGAAATGAAAG391.0958134307389715No Hit
TGTGCTGTTTACTTAAAGAGCTCATGCCTGTGGGTTAGGAAAGGAATTTC381.0677156504636134No Hit
CCACCAAATATAAGGACCCTGAAATGAAAGATGAGACATTCGAGTGCAAG381.0677156504636134No Hit
AGCCAAGGAAAGCAGCTTAGATGTTTGCTCAGTTGATGGAGTTCAGAAGAAAGCTTGTGTGAATGGAAA381.0677156504636134No Hit
TGGGTCATGGTAGAAGACAAAGTGATTGTTATGGGAGACATGACCACCAA381.0677156504636134No Hit
CACGGCTAAGACAAAGAAAGAGGCAAGCCTGTACCTAGATTCAACCACTC371.039617870188255No Hit
TAAAGAGCTCATGCCTGTGGGTTAGGAAAGGAATTTCCAATCCTACTGGA361.0115200899128969No Hit
TTTGAGATGGGATCATCCTGTTGTGGAGGATGTTTGCACTGTGAAATGCT361.0115200899128969No Hit
AGAGTACGTGCATTTGAAGCCAGCCACTAAGGACGTCAGCTCCATAACAA350.9834223096375386No Hit
CCATCAGTGTCTGCAACATGATAACTTGCACCTGGTGCTTTGACAACTGGATCAACCTGAAGTGCTACTC340.9553245293621804No Hit
ATAATGATCAATTCCGCAGATTCTTGCACTAACACTCAGAATCTAAAGGG340.9553245293621804No Hit
AGAAGACAAAGTGATTGTTATGGGAGACATGACCACCAAATATAAGGACCCTGAAATGAAAGATGAG340.9553245293621804No Hit
TGGGATGGACTGAATGTTCCCAAGTAGAAGGAGGGGTTACCAACGGCTGT340.9553245293621804No Hit
AGGAAAGTCATCGGTCTATGAGGAAAAGAAAGTGGAGTGCAATGCCATCA340.9553245293621804No Hit
TTATACTCATGATGATTGCGACTATTGAAGTTGACTCTTCCATTGACTCA330.9272267490868222No Hit
TCACGGCTAAGACAAAGAAAGAGGCAAGCCTGTACCTAGATTCAACCACT330.9272267490868222No Hit
TTTGAATGGAAGATTAGAGTACGTGCATTTGAAGCCAGCCACTAAGGACG330.9272267490868222No Hit
AAGTGATTGTTATGGGAGACATGACCACCAAATATAAGGACCCTGAAATGAAAGATGAGACATTCGA320.899128968811464No Hit
TGTTTACTTAAAGAGCTCATGCCTGTGGGTTAGGAAAGGAATTTCCAATCCTACTGGAAGGGCATACCGA320.899128968811464No Hit
ATGGATCCAAAACAACTGACATCCATTGTCAAGATGGCTCTTCTTCAGGC310.8710311885361057No Hit
TAGAGTAATAGGAAGAGCCTGTGGATCAGGAGTGTCTAGAGGATACATGA310.8710311885361057No Hit
TGAAACTGAAACCCTCATGATGAACAATGAAGGTTATTTAGTGGCTAAAT310.8710311885361057No Hit
TTCGAGTGCAAGAGCGACAACGCGACCTACAAAGTGCAAGATTGCGTGATTGAAGGAGTCTCAGACTGCA310.8710311885361057No Hit
ACGTGCATTTGAAGCCAGCCACTAAGGACGTCAGCTCCATAACAATTGATGACTCCCCGAAGAA300.8429334082607475No Hit
TTCTTTATACTCATGATGATTGCGACTATTGAAGTTGACTCTTCCATTGA290.8148356279853891No Hit
TATAGATCAAGATATGGCTTCACTCAAACTAACAATATTATCAGTGGATC290.8148356279853891No Hit
GGATGTTTGCACTGTGAAATGCTCAGGAGGAAAGACGACCTTCGATTTGAATGGAAGATTAGAGTACGTGC280.7867378477100309No Hit
GAGACAGTCACCACTTTTGACCATCGGCTCTGGAGAGGCTGCACCTGCAAGAAGACATTCAGCAGAATGGGTTT280.7867378477100309No Hit
GTAGAAGACAAAGTGATTGTTATGGGAGACATGACCACCAAATATAAGGACCCTGAAATGAAAGATGA280.7867378477100309No Hit
ATTGATGACTCCCCGAAGAACATGACTTTCCTTGATCAGCTAAAAGACAT270.7586400674346727No Hit
AAGTGGAAGGAGGGGTTACCAACGGCTGTGCTGTTTACTTAAAGAGCTCA270.7586400674346727No Hit
GTTCACACCTCAGGCATGGGGCAATCAGCTTGCCTTGGTGGAAAGAGATGGCATTG270.7586400674346727No Hit
ATCCCCAAATGTGTCTAGTAGAGCAGACTCTAGAGCAGAAGCCAGAGTTTCAAGAGCTTTGCTAAAC260.7305422871593144No Hit
TCATCACGGCTAAGACAAAGAAAGAGGCAAGCCTGTACCTAGATTCAACC260.7305422871593144No Hit
CAACGAGACTGGTTGTTTCACCTCTGGGACAAACGTCTTGTTTGAGGTTG260.7305422871593144No Hit
GTGCAAGATTGCGTGATTGAAGGAGTCTCAGACTGCAATGGAGATGAGTTGTTTTGCCAATACAAGAA230.6462489463332397No Hit
TTAGTGAGTCCCTATGAAGTTACTTCTAGGGATCCTTTCCCCATCACAACTGAAACTGAAACCCTCA230.6462489463332397No Hit
ACCATCGGCTCTGGAGAGGCTGCACCTGCAAGAAGAC220.6181511660578815No Hit
CCATCGGCTCTGGAGAGGCTGCACCTGCAAGAAGACATTCAGCAGAATGGGTTTTGAGAAA220.6181511660578815No Hit
CAATTGATGACTCCCCGAAGAACATGACTTTCCTTGATCAGCTAAAAGACATATGTA220.6181511660578815No Hit
AAGTGATTGTTATGGGAGACATGACCACCAAATATAAGGACCCTGAAATGAAAGATGAGACATTCGAGTGC210.5900533857825232No Hit
GAGATCATCACGGCTAAGACAAAGAAAGAGGCAAGCCTGTACCTAGATTCAACCACTCAGAA200.561955605507165No Hit
AAGTGTTAAAACAAGAGACAGTCACCACTTTTGACCATCGGCTCTGGAGA200.561955605507165No Hit
TCAAGGAGAACTGTGCCAACTGTGATGTCAAGTGTGTGAATGGAGTAGTGAGAATAGACTTTCACCGAGA200.561955605507165No Hit
ATAACAATTGATGACTCCCCGAAGAACATGACTTTCCTTGATCAGCTAAAAGAC200.561955605507165No Hit
AGACAAAGTGATTGTTATGGGAGACATGACCACCAAATATAAGGACCCTGAAATGAAAGATGA190.5338578252318067No Hit
CACCAAATATAAGGACCCTGAAATGAAAGATGAGACATTCGAGTGCAAGAGCGACAACGCGAC190.5338578252318067No Hit
ACGCGACCTACAAAGTGCAAGATTGCGTGATTGAAGGAGTCTCAGACTGC180.5057600449564484No Hit
GTGCCAACTGTGATGTCAAGTGTGTGAATGGAGTAGTGAGAATAGACTTTCACCGAGAG180.5057600449564484No Hit
GTGATGTCAAGTGTGTGAATGGAGTAGTGAGAATAGACTTTCACCGAGAA180.5057600449564484No Hit
GATTGCGTGATTGAAGGAGTCTCAGACTGCAATGGAGATGAGTTGTTTTGCCAATA180.5057600449564484No Hit
CACCACTTTTGACCATCGGCTCTGGAGAGGCTGCACCTGCAAGAAGAC170.4776622646810902No Hit
ACAGTGATCAAGGAGAACTGTGCCAACTGTGATGTCAAGTGTGTGAATGGAGTAGTGAGAAT170.4776622646810902No Hit
ATCATCACGGCTAAGACAAAGAAAGAGGCAAGCCTGTACCTAGATTCAACC170.4776622646810902No Hit
AGACATGACCACCAAATATAAGGACCCTGAAATGAAAGATGAGACATTCGAG170.4776622646810902No Hit
TAAGGACCCTGAAATGAAAGATGAGACATTCGAGTGCAAGAGCGACAACGCGACCTAC160.449564484405732No Hit
AACAATTGATGACTCCCCGAAGAACATGACTTTCCTTGATCAGCTAAAAGAC160.449564484405732No Hit
AAAGTGATTGTTATGGGAGACATGACCACCAAATATAAGGACCCTGAAATGAAAG160.449564484405732No Hit
AGTGATTGTTATGGGAGACATGACCACCAAATATAAGGACCCTGAAATGAAAGATGAGACAT160.449564484405732No Hit
CACGGCTAAGACAAAGAAAGAGGCAAGCCTGTACCTAGATTCAACCACT150.42146670413037374No Hit
CATCGGCTCTGGAGAGGCTGCACCTGCAAGAAGAC150.42146670413037374No Hit
CTGTAAATAATGTAAATATTAGATTTTAACAAACTTGTACATATGTAAAAAA150.42146670413037374No Hit
TAACAATTGATGACTCCCCGAAGAACATGACTTTCCTTGATCAGCA140.39336892385501543No Hit
TAACAATTGATGACTCCCCGAAGAACATGACTTTC140.39336892385501543No Hit
CTGTTGGATTGGATATCTAAGGGGCTGATCAGCATGTTGGTTACTG140.39336892385501543No Hit
GCAATTGTCATAGGGTCATTCTACCTCACAGTCTCCTGTCTAATGTGGTT140.39336892385501543No Hit
ATCAGGATGTTATAGCTGTCTGACTGGAGCCATAGTGG130.3652711435796572No Hit
AGACAGATTCCTGGAATAGCTAGAGCATCCAACAGATT130.3652711435796572No Hit
CTGTTGTGGAGGATGTTTGCACTGTGAAATGCTCAGGAGGAAAGACGAC120.337173363304299No Hit
CACCACTTTTGACCATCGGCTCTGGAGAGGCTGCACCTGCAAGAAGACATTCAGC120.337173363304299No Hit
TTTTGACCATCGGCTCTGGAGAGGCTGCACCTGCAAGAAGAC120.337173363304299No Hit
ATTGATGACTCCCCGAAGAACATGACTTTCCTTGATCAGCTAAAAG120.337173363304299No Hit
TTTGACCATCGGCTCTGGAGAGGCTGCACCTGCAA110.3090755830289407No Hit
TAAAGGCCTCTGAGGCTCTCCCAAAGAAAATAATATAATC110.3090755830289407No Hit
CGGCTAAGACAAAGAAAGAGGCAAGCCTGTACCTAGATTCAA110.3090755830289407No Hit
AAAGTGATTGTTATGGGAGACATGACCACCAAATATAAGGACC100.2809778027535825No Hit
ATAAGGACCCTGAAATGAAAGATGAGACATTCGAGTGCAAGAGCGACAACGCGACCT100.2809778027535825No Hit
CAATTGGAGTCAAATTACCTGTAAATAATGTAAAAAA100.2809778027535825No Hit
TGCAAGATTGCGTGATTGAAGGAGTCTCAGACTGCAATGGAGATGAGTTGT100.2809778027535825No Hit
CCAAAGATAGTAAGGCAGCCATCGAACTGGCGCCAGAATCCATG100.2809778027535825No Hit
CAATTGATGACTCCCCGAAGA100.2809778027535825No Hit
AATTGATGACTCCCCGAAGAACATGACTTTCCTTGATCAGCTAAAAGACA100.2809778027535825No Hit
ACAAATTCTATAGATCAAGATATGGCTTCACTCAAACTAACAAT100.2809778027535825No Hit
ATGTGTCTAGTAGAGCAGACTCTAGAGCAGAAGCCAGAGTTTCAAGA100.2809778027535825No Hit
GAAAATTGCAGATACGGAGATTCAGGAACAGTCACTTGCACAAC100.2809778027535825No Hit
CATAACAATTGATGACTCCCCGAAGAACATGACT100.2809778027535825No Hit
CCACCAAATATAAGGACCCTGAAATGAAAGATGAGACATTCGAGT90.2528800224782242No Hit
ATATAAGGACCCTGAAATGAAAGATGAGACATTCGAGTGCAAGAGCGACAACGCG90.2528800224782242No Hit
GACTCCCCGAAGAACATGACTTTCCTTGATCAGCTAAAAGACATATGTAGATTTAAC90.2528800224782242No Hit
TTGACCATCGGCTCTGGAGAGGCTGCACCTGCAAGAAGA90.2528800224782242No Hit
TGACCACCAAATATAAGGACCCTGAAATGAAAGAT90.2528800224782242No Hit
ACTCTAGTTAGGCCTCCTTCAAGACAGA90.2528800224782242No Hit
CAAACAGGGTTAGACTTCATGTTCCAAGCCACTCAGAAACCTACAG80.224782242202866No Hit
AGGAGAACTGTGCCAACTGTGATGTCAAGTGTGTGAATGGAGTAGTGA80.224782242202866No Hit
TCAGCTCCATAACAATTGATGACTCCCCGAAGAACATGACTTTCC80.224782242202866No Hit
CCGAAGAACATGACTTTCCTTGATCAGCTAAAAGAA80.224782242202866No Hit
ACAGTCACTTGCACAACTTCCGAAACAACGAGA70.19668446192750771No Hit
GGCTAGGAAAGTCATCGGTCTATGAGGAGAAGAAA70.19668446192750771No Hit
CCTCTGAGGCTCTCCTAAAGAAAATAAAAA70.19668446192750771No Hit
AATATAAGGACCCTGAAATGAAAGATGAGAA70.19668446192750771No Hit
CTTTAGATCTGACTTACTCAGGCATGATTCCC70.19668446192750771No Hit
AAGACGATGCAGTTGGATCCATCTCTTGCCTCCAAACA70.19668446192750771No Hit
TAACAATTGATGACTCCCCGA70.19668446192750771No Hit
AACAATTGATGACTCCCCGAAGAACATGACTTTCCTTGATCAGCTAAAAGACATATG60.1685866816521495No Hit
ACATTGGGAGAAATTCAGTGTCCGACAGTTGGAGA60.1685866816521495No Hit
TAACAATTGATGACTCCCCGAAGAACATGACTTTCCTTGATCAGCT60.1685866816521495No Hit
TCAAGGAGAACTGTGCCAACTGTGATGTCA60.1685866816521495No Hit
TAACAATTGATGACTCCCCGAAGAACATGACTT50.14048890137679124No Hit
CTCTGAGGCTCTCCTAAAGAAAATAAAAGAA50.14048890137679124No Hit
ACAGGGCTAATGTGAAAGGAGAGATCATC50.14048890137679124No Hit
GATGACTCCCCGAAGAACATGACTTTCCTTGA50.14048890137679124No Hit
CCATAACAATTGATGACTCCCCGAAGAACATGACTTTCC40.112391121101433No Hit
CTCTGAGGCTCTCCTAAAGAAAATAAT40.112391121101433No Hit
CAATTGATGACTCCCCGA40.112391121101433No Hit
CAATTGATGACTCCCCGAAGAACATGACTTTCCTTGATCAGCTAAAAG40.112391121101433No Hit
ACGTGCATTTGAAGC40.112391121101433No Hit
GAATAGACTTTCACCGAGAGGCTCACGCAA40.112391121101433No Hit
CTCCATAACAATTGATGACTCCCCGAAGAA40.112391121101433No Hit
TAGATCAAGATATGGCTTCACTCAAACTAACAA40.112391121101433No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)