FastQCFastQC Report
Mon 20 Feb 2023
prunned.4.R2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenameprunned.4.R2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22766151
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCAATCATGTTTTGCAGCCACCACCAAGTGATCGTGGCTAGAGAAGTC449420.19740710671733663No Hit
AAAAAAACAAAAACGTTTCTAATCTTTTTCACTCATTCCATGGCTCGTTC336030.1476007077349175No Hit
CAACTATGAAGATGTTTATGATGTTAGTGGTGGCTGCAATCATGTTTTGC307330.13499427285710264No Hit
GGAAATTCCGTGTTACTTACCATGGCCATTTCCCTTTCCGCGACCATATC228980.10057914488927004No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)