FastQCFastQC Report
Tue 24 Jan 2023
C.Las120.2.R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC.Las120.2.R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45385407
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATTGAGGGGGACTATCCAAGTACGAGCGAGCCAACTGGGAACCGA2627070.5788358359329024No Hit
GTTCTATTGAGGGAGACTATCCAAGTACGAGCGAGCCAACTGGGAACCGA937600.20658622715446837No Hit
CCCTACTCTAATCAAAAGAAAATGCGTTTCGCCTTTTGAAGCGCCAGTCG823370.1814173441256129No Hit
GTTTGTTCTATTGAGGGGGACTATCCAAGTACGAGCGAGCCAACTGGGAA816390.1798794048492283No Hit
CCCGCCTACACAAAGACCAAAATCTTACCCATTTGTAGATGGGGCTTCAA734720.1618846339749691No Hit
GGCGATAATAAATACAGAAGTTGCGGTCAATAAAGTAGGGATCATCAAAA695110.1531571590841964No Hit
GGTCAGTTCACCGGGAGGATCGCCCTCCCAATTCGAAGTTTTTTCCTGGA674710.14866232223057954No Hit
GCACGATTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATC635120.1399392540426045No Hit
GGTCAATAAAGTAGGGATCATCAAAACACCAAACCAACCAATGTAAAGGC538130.11856894882533499No Hit
GTTCTATTGAGAGGGACCATCCAGGTATGAGCGGACTGACTGGGAACCGA530300.1168437246800497No Hit
GCGGTCAATAAAGTAGGGATCATCAAAACACCAAACCAACCAATGTAAAG506170.11152703775466857No Hit
CTCATAAGGACCGCCATTGTATAACCATTCATCAACGGATGCCGCTTCCC485010.10686474619474053No Hit
CCGGATTTTCAAGGGCCGCCGGGGGCGCACCGGACACCGCGCGACGTGCG482030.10620814747788865No Hit
GCACTGAATAGGGAGCCGCCGAATACACCAGCTACGCCTAACATGTGGAA461440.101671446947694No Hit
GCCTAACATGTGGAATGGGTGCATAAGGATGTTGTGCTCAGCCTGGAATA457300.10075925946857764No Hit
GGGGGACTATCCAAGTACGAGCGAGCCAACTGGGAACCGACCCGTTATCC457230.10074383600878582No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)