FastQCFastQC Report
Tue 24 Jan 2023
C.Las120.2.R2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC.Las120.2.R2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45385407
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTGATGATCCCTACTTTATTGACCGCAACTTCTGTATTTATTATCGC782780.17247394079775466No Hit
GGGCCGGCGATGCGCCCCGGTCGGATGCGGAACGGCGAGAGCCGGTCCGC641320.14130533190988018No Hit
GCCCAATCCAAGTAATGAGTGATCCACGTGGTCCGGGCGAAGGTCTGGAT554540.12218464847081793No Hit
GTGGGAACTTAGTTTCCGTCTGGGTATGCGTCCTTGGATTGCTGTTGCAT549930.12116890347595649No Hit
GTCTGGATCACTTGGATGCTAAAGTGGAGGGAACCGCGTGAGGCTGGTTT519120.11438037781615575No Hit
CTCGCTTTTTGCAGAATTTGTATGCTTGGGAGTCCCTGATGATTAAATAA485490.10697050706188445No Hit
ATTCGGTCAAGAGGAAGAAACTTATAATATCGTAGCTGCTCACGGTTATT484140.10667305462304216No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)