FastQCFastQC Report
Tue 24 Jan 2023
C.Las180.1.R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC.Las180.1.R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45914298
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGG1277670.2782727942393892No Hit
GGCTGATCTTCCCCAAGAGCTCACATCGACGGGAAGGTTTGGCACCTCGA1261870.2748316003873129No Hit
CCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGA1187040.25853384494738435No Hit
GTCTGGAGAAGCGTCCTCAGCGACGGACCGGGCCTAAGTTCCCTGGAAAG1146810.2497718684493445No Hit
GTCCGAACCGACTGATGTTGAAAAATCAGCGGATGAGTTGTGGTTAGGGG1051750.22906807809628277No Hit
GGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATG962760.2096863160142403No Hit
GTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGGATGGGTCGGCCGGTCC905180.19714556019129378No Hit
GGGGCGTAGGCCTCCCAAAAGGTAACGGAGGCGTGCAAAGGTTTCCTCGG807210.17580798033762815No Hit
GTCTGACTTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTG801090.17447506221264672No Hit
GCCCTTTGTACACACCGCCCGTCGCTCCTACCGATTGAATGATCCGGTGA789420.17193337029785363No Hit
GTCCCAAGGCTCGCTTCGGCGGGTCGATCCGGGCGGAGGACATTGTCAGG787570.17153044570124976No Hit
GGCCTATCGATCCTTTAGACCTTCGGAATTTGAAGCTAGAGGTGTCAGAA771750.16808489590758854No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCGG736990.16051426943302063No Hit
GGGGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAATGCCGCGTGGA723580.15759361060034066No Hit
GCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTG708030.15420686601807568No Hit
GCCCTATCAACTTTCGATGGTAGGATAGTGGCCTACCATGGTGGTAACGG703220.1531592620669056No Hit
GTTCGAGTGTGAGCATGCCTGTCGGGACCCGAAAGATGGTGAACTATGCC666070.14506810057294137No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGTGTT653570.14234563708237466No Hit
GTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGTGAGCTGGGTT650480.14167264410750655No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAA620710.13518882505837287No Hit
GTCGACGCGGGCTCTGCCCGTTGCTCTGATGATTCATGATAACTCGACGG616510.13427407732554247No Hit
GCTTGAAATTGTCGGGAGGGAAGCGGATGGGGGCCGGCGATGCGTCCTGG604020.13155379180576823No Hit
GTCCGACTTTGTGAAATGACTTGAGAGGTGTAGGATAAGTGGGAGCTTCG598600.13037333163625847No Hit
GTTCTATTGAGGGGGACTATCCAAGTACGAGCGAGCCAACTGGGAACCGA589720.1284392935725599No Hit
GGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGAACCGGAAGCCGG586930.1278316397214654No Hit
GTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGTGTTGGATTGT585140.12744178294961628No Hit
GTCGTTGGCTAAGTCCGTTCGGCGGAAGCGTCGTTCGGACCGCCTTGAAT583990.12719131630848413No Hit
GTTGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGA559770.12191627104916208No Hit
GGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTC557370.12139355805897327No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAA556150.12112784562229395No Hit
CCCAAGGCTCGCTTCGGCGGGTCGATCCGGGCGGAGGACATTGTCAGGTG549030.11957713041806715No Hit
GTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAG542980.11825945808863288No Hit
GGGAGCTTCGGCGCAAGTGAAATACCACTACTTTTAACGTTATTTTACTT540550.1177302111860667No Hit
CCTGCCCTTTGTACACACCGCCCGTCGCTCCTACCGATTGAATGATCCGG539770.11756032946425533No Hit
GGGGAAACTTACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCT537380.11703979444485897No Hit
GGGGAGTTTGGCTGGGGCGGCACATCTGTTAAAAGATAACGCAGGTGTCC531620.11578528326840584No Hit
CCGGGCTAGAAGCGACGCATGCGCCCGCCGCCCGATTGCCGACCCTCAGT521660.1136160243591223No Hit
GGCAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGATTGGC508820.11081950986161218No Hit
GGGATGTCAAAATGCATCGGTAGGGGAGCGTTCCGCCTTAGGGGGAAGCA506610.11033817831647998No Hit
GCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGAACCGGAAGCCGGG495390.10789449508734729No Hit
GGGAGTTTGGCTGGGGCGGCACATCTGTTAAAAGATAACGCAGGTGTCCT492620.10729119717783771No Hit
GTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTCCGTCCAA483980.10540943041315802No Hit
GGCGTGCAAAGGTTTCCTCGGGCCGGACGGAGATTGGCCCTCGAGTGCAA480030.10454913195013893No Hit
GTGTGAATCAGCAAGGACCACCTTGCAAGGCTAAATACTCCTGGGTGACC478940.10431173313376152No Hit
GGGCGTAGTAAGCGACGAAATGCTTCGGGGAGTTGAAAATAAGCGTAGAT477870.104078690258969No Hit
GTAGGATAAGTGGGAGCTTCGGCGCAAGTGAAATACCACTACTTTTAACG477710.10404384272628975No Hit
GTGGGGAGTTTGGCTGGGGCGGCACATCTGTTAAAAGATAACGCAGGTGT474510.10334689207270467No Hit
GTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGT473310.10308553557761026No Hit
GGCTCATTAAATCAGTTATAGTTTGTTTGATGGTAACTACTACTCGGATA470800.10253886490870447No Hit
GCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACG467130.10173954962787408No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC467120.10173737165708163No Hit
GGGGGAAGCAACCGCGCGAGCGGCGGTGGACGAAGCGGAAGCGAGAATGT466530.1016088713803269No Hit
GGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGA463690.10099032767527012No Hit
GGGGAGTTGAAAATAAGCGTAGATCCGGAGATTCCCGAATAGGTTAACCT460630.1003238686127794No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)