FastQCFastQC Report
Tue 24 Jan 2023
C.Las180.1.R2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC.Las180.1.R2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45914298
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGAATCTCAGTGGATCGTGGCAGCAAGGCCACTCTGCCACTTACAATA1157340.25206527169379783No Hit
CTCATCTTGGGGTGGGCTTACTACTTAGATGCTTTCAGCAGTTATCCGCT853230.18583100192449856No Hit
CCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTT805410.17541594559498655No Hit
AGAACATCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCTTGGC712660.1552152664949816No Hit
CGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAGTGG698020.15202671725482986No Hit
GGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAA666260.14510948201799798No Hit
GTTTAGTGGACTTCTCGCGACGTCGCGGGCGGCGAACCACCCACGTCGCC657380.14317544395429938No Hit
CTTAGGCCCTGACTCACCCTCCGTGGACGAACCTTGCGGAGGAACCCTTG657190.14313406250924277No Hit
CCTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTA624740.1360665472877316No Hit
GTCCTCTCAATGCTCTAACGCCCACACCGGATATGGACCGAACTGTCTCA555260.12093400622176562No Hit
GTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGGATCCCCT525030.11435000051617907No Hit
ATCGACTACGCCTTTCGGCCTGATCTTAGGCCCTGACTCACCCTCCGTGG524420.11421714429783941No Hit
GTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTC473260.103074645723648No Hit
GTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAGTGGACTTCTCGCG471840.10276537387111963No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)