FastQCFastQC Report
Tue 24 Jan 2023
C.Las180.2.R2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC.Las180.2.R2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45270872
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCTTTTTGCAGAATTTGTATGCTTGGGAGTCCCTGATGATTAAATAA1031860.22793022409641236No Hit
GTTTTGATGATCCCTACTTTATTGACCGCAACTTCTGTATTTATTATCGC830640.183482217881732No Hit
CTTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATTGCTGCTCCT646320.14276729637547073No Hit
TGATGATTAAATAAACCAAGATTTTACCATGACTGCAATTTTAGAGAGAC629940.13914907581192604No Hit
GTTTTTCTGATGGTATGCCTCTAGGAATCTCTGGTACTTTCAATTTCATG626920.13848198020130914No Hit
GTTTGCCGACCCGCAGTAGGGACCTCCCGGTCCCCAGAGGCACGTGTCGT588530.13000191381336768No Hit
ATTCGGTCAAGAGGAAGAAACTTATAATATCGTAGCTGCTCACGGTTATT567690.1253985123149384No Hit
CTTTATTGACCGCAACTTCTGTATTTATTATCGCCAGATCGGAAGAGCGT560300.12376611610220363No Hit
ATCTGGTTCACTGCTTTAGGTATTAGCACTATGGCTTTCAACTTAAATGG547470.12093206422001325No Hit
GTGGGAACTTAGTTTCCGTCTGGGTATGCGTCCTTGGATTGCTGTTGCAT526560.11631320024054319No Hit
TCTCGCTTTTTGCAGAATTTGTATGCTTGGGAGTCCCTGATGATTAAATA511730.11303736318575883No Hit
CTTCCAATATGCTAGTTTCAACAATTCTCGTTCTTTACATTTCTTCCTGG502130.11091679435730772No Hit
GCACAACATCCTTATGCACCCATTCCACATGTTAGGCGTAGCTGGTGTAT467080.10317450920759821No Hit
GAATTTGTATGCTTGGGAGTCCCTGATGATTAAATAAACCAAGATTTTAC460900.10180939302428281No Hit
CTTAGTTTCCGTCTGGGTATGCGTCCTTGGATTGCTGTTGCATATTCAGC460700.10176521450702342No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)