FastQCFastQC Report
Tue 24 Jan 2023
C.h120.2.R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC.h120.2.R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46362841
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGCCTACACAAAGACCAAAATCTTACCCATTTGTAGATGGGGCTTCAA1844120.39775819605187696No Hit
GTTCTATTGAGGGGGACTATCCAAGTACGAGCGAGCCAACTGGGAACCGA1290780.27840830547895024No Hit
GCACGATTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATC1174780.25338826841953016No Hit
GGCGATAATAAATACAGAAGTTGCGGTCAATAAAGTAGGGATCATCAAAA1112250.23990117430465488No Hit
CCCAGACGGAAACTAAGTTCCCACTCACGACCCATGTAACAAGCTACACC1100150.2372913256113878No Hit
CTCATAAGGACCGCCATTGTATAACCATTCATCAACGGATGCCGCTTCCC1050330.22654565107431615No Hit
CCGCCATTGTATAACCATTCATCAACGGATGCCGCTTCCCATATCGGGTA845530.18237234426596075No Hit
AGCACGATTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTAT831060.17925131033277275No Hit
GGTCAATAAAGTAGGGATCATCAAAACACCAAACCAACCAATGTAAAGGC815120.17581321213684897No Hit
GTTCACGAATACCGTCAATATCTACTGGAGGAGCAGCAATGAAGGCGATA737740.1591231218984186No Hit
CCCTACTCTAATCAAAAGAAAATGCGTTTCGCCTTTTGAAGCGCCAGTCG715800.15439088385459382No Hit
CAGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGG702610.15154593308895803No Hit
GCACTGAATAGGGAGCCGCCGAATACACCAGCTACGCCTAACATGTGGAA653450.14094261393515553No Hit
GCCTAACATGTGGAATGGGTGCATAAGGATGTTGTGCTCAGCCTGGAATA648930.13996769524973676No Hit
GCCGCTTCCCATATCGGGTAAAAATGCAAACCTATAGCTGCAGAAGTAGG641190.1382982548459444No Hit
GCCAGGAAGAAATGTAAAGAACGAGAATTGTTGAAACTAGCATATTGGAA640690.13819040985861933No Hit
GTCAATATCTACTGGAGGAGCAGCAATGAAGGCGATAATAAATACAGAAG633020.1365360677530525No Hit
CTACGATATTATAAGTTTCTTCCTCTTGACCGAATCTGTAACCTGCATTA627260.1352936934990675No Hit
GCGGTCAATAAAGTAGGGATCATCAAAACACCAAACCAACCAATGTAAAG615190.1326903155050399No Hit
GGTCAGTTCACCGGGAGGATCGCCCTCCCAATTCGAAGTTTTTTCCTGGA613300.1322826614529511No Hit
GCTAGGTCTAGAGGGAAGTTATGAGCATTACGTTCATGCATAACTTCCAT604230.13032635338287402No Hit
CCAGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGG583150.12577960871724836No Hit
CGAGAATTGTTGAAACTAGCATATTGGAAGATCAATCGGCCAAAATAACC577140.12448331196960082No Hit
CATGTAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG556180.11996245010093319No Hit
CAGAAATAATATTGTTTCCATAAAGTAGAGATCCAGAAACAGGTTCACGA555090.11972734802856452No Hit
CCAGAAACAGGTTCACGAATACCGTCAATATCTACTGGAGGAGCAGCAAT539700.11640787931869835No Hit
AGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGGT535430.11548688312694212No Hit
CCAGACGGAAACTAAGTTCCCACTCACGACCCATGTAACAAGCTACACCA515730.11123779062633371No Hit
AGAAAAACTTCCTTGACCGATTGGGTAGATCAAGAAAACAGCAGTCGCTG499690.10777812343294493No Hit
GCCTACACAAAGACCAAAATCTTACCCATTTGTAGATGGGGCTTCAATAG489190.10551337869911812No Hit
GTTATTTTCAAAAACCCGCCTACACAAAGACCAAAATCTTACCCATTTGT476680.10281509711624445No Hit
GGGTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATGAAATTGAA464200.1001232862326103No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)