FastQCFastQC Report
Tue 24 Jan 2023
C.h120.3.R2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC.h120.3.R2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37789146
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTGATGATCCCTACTTTATTGACCGCAACTTCTGTATTTATTATCGC955440.25283450438387783No Hit
GTGGGAACTTAGTTTCCGTCTGGGTATGCGTCCTTGGATTGCTGTTGCAT762710.2018330872044581No Hit
CTCGCTTTTTGCAGAATTTGTATGCTTGGGAGTCCCTGATGATTAAATAA643610.17031610081900236No Hit
ATTCGGTCAAGAGGAAGAAACTTATAATATCGTAGCTGCTCACGGTTATT554300.14668233042366186No Hit
TGATGATTAAATAAACCAAGATTTTACCATGACTGCAATTTTAGAGAGAC542620.14359149582263647No Hit
GCACAACATCCTTATGCACCCATTCCACATGTTAGGCGTAGCTGGTGTAT482700.12773509091737612No Hit
GTTAGGCGTAGCTGGTGTATTCGGCGGCTCCCTATTCAGTGCTATGCATG446960.11827734873923852No Hit
GTTTTTCTGATGGTATGCCTCTAGGAATCTCTGGTACTTTCAATTTCATG444520.11763166068902431No Hit
ATCTGGTTCACTGCTTTAGGTATTAGCACTATGGCTTTCAACTTAAATGG424040.11221211508722637No Hit
GGGAAACCACAGAAAATGAATCTGCTAATGCAGGTTACAGATTCGGTCAA423430.11205069307467283No Hit
GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTACATGGGTCGTG421420.11151879431199635No Hit
CATGACTGCAATTTTAGAGAGACGCGAAAGCGAACGCCTATGGGGTCGCT419040.11088898383678743No Hit
GTTGCATATTCAGCTCCTGTTGCAGCAGCGACTGCTGTTTTCTTGATCTA398550.10546679197248861No Hit
GTTATTTTGGCCGATTGATCTTCCAATATGCTAGTTTCAACAATTCTCGT398260.10539005035996316No Hit
GCTTGTTACATGGGTCGTGAGTGGGAACTTAGTTTCCGTCTGGGTATGCG383760.1015529697336902No Hit
ATTCAGCTCCTGTTGCAGCAGCGACTGCTGTTTTCTTGATCTACCCAATC377980.10002343000818277No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)