FastQCFastQC Report
Tue 24 Jan 2023
C.h180.1.R2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC.h180.1.R2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46277790
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCTTTTTGCAGAATTTGTATGCTTGGGAGTCCCTGATGATTAAATAA1162540.2512090573037304No Hit
GTTTTGATGATCCCTACTTTATTGACCGCAACTTCTGTATTTATTATCGC945510.20431183079399426No Hit
TGATGATTAAATAAACCAAGATTTTACCATGACTGCAATTTTAGAGAGAC680060.1469517018855049No Hit
GTGGGAACTTAGTTTCCGTCTGGGTATGCGTCCTTGGATTGCTGTTGCAT633140.13681292905300793No Hit
ATCTGGTTCACTGCTTTAGGTATTAGCACTATGGCTTTCAACTTAAATGG608570.13150368675772978No Hit
ATTCGGTCAAGAGGAAGAAACTTATAATATCGTAGCTGCTCACGGTTATT597430.12909648451233302No Hit
GTTTTTCTGATGGTATGCCTCTAGGAATCTCTGGTACTTTCAATTTCATG590240.12754282345807783No Hit
CTTAGTTTCCGTCTGGGTATGCGTCCTTGGATTGCTGTTGCATATTCAGC552380.1193617932057689No Hit
GTTTGCCGACCCGCAGTAGGGACCTCCCGGTCCCCAGAGGCACGTGTCGT548440.11851041287840236No Hit
CTTCCAATATGCTAGTTTCAACAATTCTCGTTCTTTACATTTCTTCCTGG545610.11789888843006548No Hit
TCTCGCTTTTTGCAGAATTTGTATGCTTGGGAGTCCCTGATGATTAAATA543480.11743862444598153No Hit
GAATTTGTATGCTTGGGAGTCCCTGATGATTAAATAAACCAAGATTTTAC514480.1111721194983598No Hit
CTTCTGTAACTGGATAACTAGCACTGAAAACCGCCTTTACATTGGTTGGT501330.10833058363417958No Hit
GCACAACATCCTTATGCACCCATTCCACATGTTAGGCGTAGCTGGTGTAT484270.10464415003395798No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)