FastQCFastQC Report
Tue 24 Jan 2023
C.h180.2.R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC.h180.2.R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43905197
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGCCTACACAAAGACCAAAATCTTACCCATTTGTAGATGGGGCTTCAA2302980.5245347151044556No Hit
GGCGATAATAAATACAGAAGTTGCGGTCAATAAAGAGATCGGAAGAGCAC1428500.32536011625229694No Hit
GTTCTATTGAGGGGGACTATCCAAGTACGAGCGAGCCAACTGGGAACCGA1252680.28531474303600096No Hit
GGCGATAATAAATACAGAAGTTGCGGTCAATAAAGTAGGGATCATCAAAA1196410.27249849260441766No Hit
GCACGATTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATC1159840.26416918252297106No Hit
CTCATAAGGACCGCCATTGTATAACCATTCATCAACGGATGCCGCTTCCC1098030.25009112247008025No Hit
CCGCCATTGTATAACCATTCATCAACGGATGCCGCTTCCCATATCGGGTA1093300.24901380125910832No Hit
CAGAAATAATATTGTTTCCATAAAGTAGAGATCCAGAAACAGGTTCACGA998450.22741043617228274No Hit
GTCAATATCTACTGGAGGAGCAGCAATGAAGGCGATAATAAATACAGAAG919290.209380679922698No Hit
CCCTACTCTAATCAAAAGAAAATGCGTTTCGCCTTTTGAAGCGCCAGTCG910930.2074765773172593No Hit
CGCCATTGTATAACCATTCATCAACGGATGCCGCTTCCCATATCGGGTAA897150.20433799670685912No Hit
GGTCAATAAAGTAGGGATCATCAAAACACCAAACCAACCAATGTAAAGGC869580.19805855785136323No Hit
CCCAGACGGAAACTAAGTTCCCACTCACGACCCATGTAACAAGCTACACC828250.1886450936548582No Hit
AGCACGATTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTAT827720.18852437901599667No Hit
CGCCTACACAAAGACCAAAATCTTACCCATTTGTAGATGGGGCTTCAATA801570.18256836428726192No Hit
GCGGTCAATAAAGTAGGGATCATCAAAACACCAAACCAACCAATGTAAAG799730.1821492795032898No Hit
CTACGATATTATAAGTTTCTTCCTCTTGACCGAATCTGTAACCTGCATTA714600.16275977534049102No Hit
CTTGACCGAATCTGTAACCTGCATTAGCAGATTCATTTTCTGTGGTTTCC707420.1611244336291214No Hit
GCCGCTTCCCATATCGGGTAAAAATGCAAACCTATAGCTGCAGAAGTAGG677160.1542323110405358No Hit
CATGTAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG676910.15417537017314828No Hit
GGTCAGTTCACCGGGAGGATCGCCCTCCCAATTCGAAGTTTTTTCCTGGA669130.15240337038004864No Hit
CGAGAATTGTTGAAACTAGCATATTGGAAGATCAATCGGCCAAAATAACC642490.14633575155123435No Hit
CAATAAAGTAGGGATCATCAAAACACCAAACCAACCAATGTAAAGGCGGT613300.13968733587506735No Hit
GTTCACGAATACCGTCAATATCTACTGGAGGAGCAGCAATGAAGGCGATA601040.13689495573838334No Hit
CAGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGG593850.1352573363923182No Hit
CACGATTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATCA588210.13397275042405574No Hit
GTTCTATTGAGGGAGACTATCCAAGTACGAGCGAGCCAACTGGGAACCGA572900.1304856917052439No Hit
CAGATACCTACTACAGGCCAAGCAGCCAGGAAGAAATGTAAAGAACGAGA572500.13039458631742387No Hit
CCAGAAACAGGTTCACGAATACCGTCAATATCTACTGGAGGAGCAGCAAT560370.12763181543178134No Hit
GCTAGGTCTAGAGGGAAGTTATGAGCATTACGTTCATGCATAACTTCCAT550720.12543389795062304No Hit
CTACTACAGGCCAAGCAGCCAGGAAGAAATGTAAAGAACGAGAATTGTTG549800.12522435555863692No Hit
GCCTACACAAAGACCAAAATCTTACCCATTTGTAGATGGGGCTTCAATAG545020.12413564617418753No Hit
CAGACGGAAACTAAGTTCCCACTCACGACCCATGTAACAAGCTACACCAA524800.11953026881988481No Hit
GTCAATAAAGTAGGGATCATCAAAACACCAAACCAACCAATGTAAAGGCG522780.11907018661139364No Hit
GCGATAATAAATACAGAAGTTGCGGTCAATAAAGTAGGGATCATCAAAAC522610.11903146682157012No Hit
ACCAGAAATAATATTGTTTCCATAAAGTAGAGATCCAGAAACAGGTTCAC518010.11798375486163973No Hit
AGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGGT514460.11717519454473692No Hit
GCACTGAATAGGGAGCCGCCGAATACACCAGCTACGCCTAACATGTGGAA512730.11678116374241526No Hit
AGAAAAACTTCCTTGACCGATTGGGTAGATCAAGAAAACAGCAGTCGCTG502030.11434409461822936No Hit
GTCAGTTCACCGGGAGGATCGCCCTCCCAATTCGAAGTTTTTTCCTGGAA493640.11243315910870413No Hit
CCAGACGGAAACTAAGTTCCCACTCACGACCCATGTAACAAGCTACACCA486070.11070898964421No Hit
GCCAGGAAGAAATGTAAAGAACGAGAATTGTTGAAACTAGCATATTGGAA466010.1061400544450353No Hit
GTTATTTTCAAAAACCCGCCTACACAAAGACCAAAATCTTACCCATTTGT464640.10582801849175166No Hit
GCCTAACATGTGGAATGGGTGCATAAGGATGTTGTGCTCAGCCTGGAATA459470.10465048135417773No Hit
GTTCTATTGAGAGGGACCATCCAGGTATGAGCGGACTGACTGGGAACCGA458850.10450926800305668No Hit
CTGGAAGTTTCCACCTTGTTGACTATGACAACAGTCGCGACTATAAACAG454050.10341600334921627No Hit
TTCACGAATACCGTCAATATCTACTGGAGGAGCAGCAATGAAGGCGATAA443320.10097210132094384No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)