FastQCFastQC Report
Tue 24 Jan 2023
C.h180.3.R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC.h180.3.R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46375935
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATTGAGGGGGACTATCCAAGTACGAGCGAGCCAACTGGGAACCGA1599000.3447908920865962No Hit
GTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGG886230.19109695578105326No Hit
GGCTGATCTTCCCCAAGAGCTCACATCGACGGGAAGGTTTGGCACCTCGA791050.17057338035340958No Hit
GTCTGGAGAAGCGTCCTCAGCGACGGACCGGGCCTAAGTTCCCTGGAAAG746100.16088085339950559No Hit
GTTCTATTGAGGGAGACTATCCAAGTACGAGCGAGCCAACTGGGAACCGA715320.15424379044864542No Hit
GGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATG693420.1495215136902361No Hit
GTCCGAACCGACTGATGTTGAAAAATCAGCGGATGAGTTGTGGTTAGGGG655300.14130173332354376No Hit
CCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGA640740.13816217398096664No Hit
GTCTGACTTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTG630730.13600372693294485No Hit
GTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGGATGGGTCGGCCGGTCC616870.13301510794337623No Hit
GCACGATTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATC614090.13241565911285672No Hit
GGGGCGTAGGCCTCCCAAAAGGTAACGGAGGCGTGCAAAGGTTTCCTCGG602110.12983242278565382No Hit
GCTTGAAATTGTCGGGAGGGAAGCGGATGGGGGCCGGCGATGCGTCCTGG582030.12550259094506666No Hit
GTCGACGCGGGCTCTGCCCGTTGCTCTGATGATTCATGATAACTCGACGG496330.10702317915530975No Hit
GTTCGAGTGTGAGCATGCCTGTCGGGACCCGAAAGATGGTGAACTATGCC494810.10669542296020554No Hit
GCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTG491830.10605284831454072No Hit
GCCGCTTCCCATATCGGGTAAAAATGCAAACCTATAGCTGCAGAAGTAGG489810.10561727758157329No Hit
CCCGCCTACACAAAGACCAAAATCTTACCCATTTGTAGATGGGGCTTCAA485360.10465772819459057No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)