FastQCFastQC Report
Tue 24 Jan 2023
M.Las10.4.R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM.Las10.4.R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43714540
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGATTTTCAAGGGCCGCCGGGGGCGCACCGGACACCGCGCGACGTGCG694610.15889678811672273No Hit
GTCCTATTGAGAGAGACCATCCAAGTATGCACGGGCCAACTGGGAACCGA648520.14835338539533988No Hit
CTGCAAAGGATTCTACCCGCCGCTCGGTGGGAATTAAGATTCAAGGCGGC571180.13066133144715694No Hit
CCCTACTCTAAAGAAAATTCGTTTCGCCTTTTGAAGCGCCAGTCGCGTAG508430.11630683978374244No Hit
CCCGACTCGCAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCGCGACGG486960.11139543044488172No Hit
GGTCAGTTCACCGGGAGGATCGCCCTCCCAATTCGAAGTTTTTTCCTGGA477320.10919021451443844No Hit
CAGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGG475430.10875786408824158No Hit
GTTCATATTAGGGAAAGGAGAGTGAGGGGAAGAGTCAACTACGTCGAACC445150.10183110699552139No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)