FastQCFastQC Report
Tue 24 Jan 2023
M.Las10.5.R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM.Las10.5.R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46162266
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTACTCTAAAGAAAATTCGTTTCGCCTTTTGAAGCGCCAGTCGCGTAG784860.17002198288966144No Hit
CCGGATTTTCAAGGGCCGCCGGGGGCGCACCGGACACCGCGCGACGTGCG776820.16828030062475702No Hit
GGTCAGTTCACCGGGAGGATCGCCCTCCCAATTCGAAGTTTTTTCCTGGA703810.1524643525948228No Hit
CTGCAAAGGATTCTACCCGCCGCTCGGTGGGAATTAAGATTCAAGGCGGC622870.13493055128619552No Hit
CCCGACTCGCAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCGCGACGG562470.1218462715846748No Hit
GTCAGTTCACCGGGAGGATCGCCCTCCCAATTCGAAGTTTTTTCCTGGAA520500.11275443020929692No Hit
CCGCCATTGTATAACCATTCATCAACGGATGCCGCTTCCCATATCGGGTA470610.10194690182669976No Hit
CTGGAAGTTTCCACCTTGTTGACTATGACAACAGTCGCGACTATAAACAG463970.100508497568122No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)