FastQCFastQC Report
Tue 24 Jan 2023
M.h10.4.R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM.h10.4.R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42018424
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGATTTTCAAGGGCCGCCGGGGGCGCACCGGACACCGCGCGACGTGCG885220.21067425089527392No Hit
CTGCAAAGGATTCTACCCGCCGCTCGGTGGGAATTAAGATTCAAGGCGGC494100.11759127377076303No Hit
CCCGACTCGCAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCGCGACGG465410.11076331658702858No Hit
CCCTACTCTAAAGAAAATTCGTTTCGCCTTTTGAAGCGCCAGTCGCGTAG438100.104263786761731No Hit
GTTCATATTAGGGAAAGGAGAGTGAGGGGAAGAGTCAACTACGTCGAACC424340.10098903281094028No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)