FastQCFastQC Report
Tue 24 Jan 2023
M.h10.5.R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM.h10.5.R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46181859
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAAGTTTACCTACTACTGTACCGGCGTGAATATGATCTCCACCAGACA772020.16716953728519243No Hit
CTACGATATTATAAGTTTCTTCCTCTTGACCGAATCTGTAACCTGCATTA742010.16067131468224352No Hit
CTTGACCGAATCTGTAACCTGCATTAGCAGATTCATTTTCTGTGGTTTCC663550.1436819596196853No Hit
CTCAGCCTGGAATACAATCATGAAGTTGAAAGTACCAGAGATTCCTAGAG637600.13806287009797505No Hit
CTCATAAGGACCGCCATTGTATAACCATTCATCAACGGATGCCGCTTCCC558260.12088296402273456No Hit
CCCAGGTATTAATTACACGACCTTGACTATCAACTACGGATTGGTTGAAA482290.10443278171197048No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)