FastQCFastQC Report
Tue 24 Jan 2023
C.Las180.1.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC.Las180.1.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45700809
Sequences flagged as poor quality0
Sequence length20-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGG1268090.27747648843590494No Hit
GGCTGATCTTCCCCAAGAGCTCACATCGACGGGAAGGTTTGGCACCTCGA1250640.2736581752852559No Hit
CCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGA1177830.2577262910159862No Hit
GTCTGGAGAAGCGTCCTCAGCGACGGACCGGGCCTAAGTTCCCTGGAAAG1138150.2490437313702696No Hit
GTCCGAACCGACTGATGTTGAAAAATCAGCGGATGAGTTGTGGTTAGGGG1036690.22684281146970509No Hit
GGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATG941250.20595915490248762No Hit
GTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGGATGGGTCGGCCGGTCC897880.19646916972520115No Hit
GGGGCGTAGGCCTCCCAAAAGGTAACGGAGGCGTGCAAAGGTTTCCTCGG802420.1755811368678397No Hit
GTCTGACTTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTG795650.1740997626540922No Hit
GTCCCAAGGCTCGCTTCGGCGGGTCGATCCGGGCGGAGGACATTGTCAGG780770.17084380278694847No Hit
GCCCTTTGTACACACCGCCCGTCGCTCCTACCGATTGAATGATCCGGTGA780640.1708153569010124No Hit
GGCCTATCGATCCTTTAGACCTTCGGAATTTGAAGCTAGAGGTGTCAGAA763850.1671414613251157No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCGG726770.15902781939812052No Hit
GGGGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAATGCCGCGTGGA715990.1566689990104989No Hit
GCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTG703740.15398852129729257No Hit
GCCCTATCAACTTTCGATGGTAGGATAGTGGCCTACCATGGTGGTAACGG697370.15259467288642525No Hit
GTTCGAGTGTGAGCATGCCTGTCGGGACCCGAAAGATGGTGAACTATGCC661140.1446670232905505No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGTGTT647680.14172178002363153No Hit
GTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGTGAGCTGGGTT645490.1412425762528624No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAA615190.13461249668468672No Hit
GTCGACGCGGGCTCTGCCCGTTGCTCTGATGATTCATGATAACTCGACGG612430.13400856864481328No Hit
GCTTGAAATTGTCGGGAGGGAAGCGGATGGGGGCCGGCGATGCGTCCTGG598260.13090796707778193No Hit
GTCCGACTTTGTGAAATGACTTGAGAGGTGTAGGATAAGTGGGAGCTTCG593860.12994518324609966No Hit
GTTCTATTGAGGGGGACTATCCAAGTACGAGCGAGCCAACTGGGAACCGA584920.12798898155172703No Hit
GGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGAACCGGAAGCCGG582550.12747039116966177No Hit
GTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGTGTTGGATTGT580810.1270896539271329No Hit
GTCGTTGGCTAAGTCCGTTCGGCGGAAGCGTCGTTCGGACCGCCTTGAAT578930.12667828265359593No Hit
GTTGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGA555260.12149894326815966No Hit
GGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTC553470.12110726530027072No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAA551490.12067401257601369No Hit
CCCAAGGCTCGCTTCGGCGGGTCGATCCGGGCGGAGGACATTGTCAGGTG544900.11923202497356228No Hit
GTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAG538740.1178841276092071No Hit
GGGAGCTTCGGCGCAAGTGAAATACCACTACTTTTAACGTTATTTTACTT536430.1173786660975739No Hit
CCTGCCCTTTGTACACACCGCCCGTCGCTCCTACCGATTGAATGATCCGG534930.11705044433677311No Hit
GGGGAAACTTACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCT534220.11689508603666075No Hit
GGGGAGTTTGGCTGGGGCGGCACATCTGTTAAAAGATAACGCAGGTGTCC529410.11584258825702626No Hit
CCGGGCTAGAAGCGACGCATGCGCCCGCCGCCCGATTGCCGACCCTCAGT516900.11310521877194778No Hit
GGCAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGATTGGC507160.11097396547181473No Hit
GGGATGTCAAAATGCATCGGTAGGGGAGCGTTCCGCCTTAGGGGGAAGCA501230.10967639544411567No Hit
GCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGAACCGGAAGCCGGG495290.1083766372713446No Hit
GGGAGTTTGGCTGGGGCGGCACATCTGTTAAAAGATAACGCAGGTGTCCT491900.10763485609193482No Hit
GTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTCCGTCCAA479630.10495000208858447No Hit
GGCGTGCAAAGGTTTCCTCGGGCCGGACGGAGATTGGCCCTCGAGTGCAA476300.10422134977960676No Hit
GTGTGAATCAGCAAGGACCACCTTGCAAGGCTAAATACTCCTGGGTGACC475910.10413601212179854No Hit
GTAGGATAAGTGGGAGCTTCGGCGCAAGTGAAATACCACTACTTTTAACG475730.10409662551050244No Hit
GGGCGTAGTAAGCGACGAAATGCTTCGGGGAGTTGAAAATAAGCGTAGAT474350.10379466149056574No Hit
GTGGGGAGTTTGGCTGGGGCGGCACATCTGTTAAAAGATAACGCAGGTGT471430.10315572312954023No Hit
GTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGT469210.10266995492355507No Hit
GGCTCATTAAATCAGTTATAGTTTGTTTGATGGTAACTACTACTCGGATA467440.10228265324581017No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC466050.10197850108080142No Hit
GGGGGAAGCAACCGCGCGAGCGGCGGTGGACGAAGCGGAAGCGAGAATGT463950.10151899061568036No Hit
GGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGA461240.10092600330116694No Hit
GCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACG458300.10028268864999743No Hit
GGGGAGTTGAAAATAAGCGTAGATCCGGAGATTCCCGAATAGGTTAACCT457830.10017984583161317No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)