FastQCFastQC Report
Tue 24 Jan 2023
C.Las180.1.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC.Las180.1.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45700809
Sequences flagged as poor quality0
Sequence length20-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGAATCTCAGTGGATCGTGGCAGCAAGGCCACTCTGCCACTTACAATA1150550.251757031259556No Hit
CTCATCTTGGGGTGGGCTTACTACTTAGATGCTTTCAGCAGTTATCCGCT846150.18514989526771836No Hit
CCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTT799890.17502753616462238No Hit
AGAACATCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCTTGGC704620.15418107806362902No Hit
CGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAGTGG694310.1519251004943917No Hit
GGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAA661240.14468890474127055No Hit
GTTTAGTGGACTTCTCGCGACGTCGCGGGCGGCGAACCACCCACGTCGCC654460.14320534238245103No Hit
CTTAGGCCCTGACTCACCCTCCGTGGACGAACCTTGCGGAGGAACCCTTG651300.1425138885396974No Hit
CCTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTA620800.1358400460700816No Hit
GTCCTCTCAATGCTCTAACGCCCACACCGGATATGGACCGAACTGTCTCA551770.12073528063802984No Hit
ATCGACTACGCCTTTCGGCCTGATCTTAGGCCCTGACTCACCCTCCGTGG521090.11402205155711795No Hit
GTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGGATCCCCT520340.11385794067671755No Hit
GTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAGTGGACTTCTCGCG469830.10280561991801938No Hit
GTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTC469450.1027224704052832No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)