FastQCFastQC Report
Tue 24 Jan 2023
C.Las180.2.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC.Las180.2.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45198793
Sequences flagged as poor quality0
Sequence length20-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGCCTACACAAAGACCAAAATCTTACCCATTTGTAGATGGGGCTTCAA2061710.45614271159851544No Hit
GTTCTATTGAGGGGGACTATCCAAGTACGAGCGAGCCAACTGGGAACCGA1503220.33257967751483986No Hit
GCACGATTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATC1044830.23116325252313707No Hit
CCGCCATTGTATAACCATTCATCAACGGATGCCGCTTCCCATATCGGGTA1038400.22974064816288345No Hit
CCCTACTCTAATCAAAAGAAAATGCGTTTCGCCTTTTGAAGCGCCAGTCG953070.2108618254474185No Hit
CTCATAAGGACCGCCATTGTATAACCATTCATCAACGGATGCCGCTTCCC912820.20195672039295384No Hit
GGCGATAATAAATACAGAAGTTGCGGTCAATAAAGTAGGGATCATCAAAA837990.18540096856126226No Hit
CGCCATTGTATAACCATTCATCAACGGATGCCGCTTCCCATATCGGGTAA823760.18225265440163413No Hit
AGCACGATTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTAT788240.1743940374690979No Hit
GGTCAATAAAGTAGGGATCATCAAAACACCAAACCAACCAATGTAAAGGC761210.168413789279727No Hit
CAGAAATAATATTGTTTCCATAAAGTAGAGATCCAGAAACAGGTTCACGA759130.16795359999989382No Hit
CGCCTACACAAAGACCAAAATCTTACCCATTTGTAGATGGGGCTTCAATA715580.15831838695338613No Hit
CCCAGACGGAAACTAAGTTCCCACTCACGACCCATGTAACAAGCTACACC699420.15474307024083586No Hit
GCGGTCAATAAAGTAGGGATCATCAAAACACCAAACCAACCAATGTAAAG665260.1471853462989598No Hit
CTTGACCGAATCTGTAACCTGCATTAGCAGATTCATTTTCTGTGGTTTCC657430.14545299915420307No Hit
CTACGATATTATAAGTTTCTTCCTCTTGACCGAATCTGTAACCTGCATTA655040.14492422397208704No Hit
GGTCAGTTCACCGGGAGGATCGCCCTCCCAATTCGAAGTTTTTTCCTGGA647440.14324276314192724No Hit
GTTCTATTGAGGGAGACTATCCAAGTACGAGCGAGCCAACTGGGAACCGA647080.14316311499734075No Hit
GTCAATATCTACTGGAGGAGCAGCAATGAAGGCGATAATAAATACAGAAG594330.13149244936695542No Hit
GGCGATAATAAATACAGAAGTTGCGGTCAATAAAG584140.1292379643854649No Hit
GCCGCTTCCCATATCGGGTAAAAATGCAAACCTATAGCTGCAGAAGTAGG580610.1284569700788249No Hit
CATGTAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG573240.12682639556326206No Hit
CGAGAATTGTTGAAACTAGCATATTGGAAGATCAATCGGCCAAAATAACC550550.12180635000585081No Hit
CACGATTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATCA522570.11561591921270996No Hit
CAGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGG512840.11346320686041328No Hit
CTACTACAGGCCAAGCAGCCAGGAAGAAATGTAAAGAACGAGAATTGTTG487380.10783031307937802No Hit
CAATAAAGTAGGGATCATCAAAACACCAAACCAACCAATGTAAAGGCGGT485100.1073258748303301No Hit
GCTAGGTCTAGAGGGAAGTTATGAGCATTACGTTCATGCATAACTTCCAT477130.10556255340712305No Hit
GTTATTTTCAAAAACCCGCCTACACAAAGACCAAAATCTTACCCATTTGT461060.10200714873071942No Hit
GTTCTATTGAGAGGGACCATCCAGGTATGAGCGGACTGACTGGGAACCGA457540.10122836687254015No Hit
GTTCACGAATACCGTCAATATCTACTGGAGGAGCAGCAATGAAGGCGATA457290.10117305566102175No Hit
CTGGAAGTTTCCACCTTGTTGACTATGACAACAGTCGCGACTATAAACAG457070.10112438179488553No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)