FastQCFastQC Report
Tue 24 Jan 2023
C.Las180.2.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC.Las180.2.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45198793
Sequences flagged as poor quality0
Sequence length20-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCTTTTTGCAGAATTTGTATGCTTGGGAGTCCCTGATGATTAAATAA1030770.22805254998734145No Hit
GTTTTGATGATCCCTACTTTATTGACCGCAACTTCTGTATTTATTATCGC794560.1757923048962834No Hit
GTTTTTCTGATGGTATGCCTCTAGGAATCTCTGGTACTTTCAATTTCATG621340.13746827265940487No Hit
TGATGATTAAATAAACCAAGATTTTACCATGACTGCAATTTTAGAGAGAC618970.1369439223742103No Hit
GTTTGCCGACCCGCAGTAGGGACCTCCCGGTCCCCAGAGGCACGTGTCGT585100.12945035943769562No Hit
CTTTATTGACCGCAACTTCTGTATTTATTATCGCC577130.12768703801448858No Hit
ATTCGGTCAAGAGGAAGAAACTTATAATATCGTAGCTGCTCACGGTTATT559810.12385507728049287No Hit
ATCTGGTTCACTGCTTTAGGTATTAGCACTATGGCTTTCAACTTAAATGG537660.1189545039399614No Hit
GTGGGAACTTAGTTTCCGTCTGGGTATGCGTCCTTGGATTGCTGTTGCAT521480.11537476233048967No Hit
CTTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATTGCTGCTCCT511360.11313576448822428No Hit
TCTCGCTTTTTGCAGAATTTGTATGCTTGGGAGTCCCTGATGATTAAATA508180.11243220587771005No Hit
CTTCCAATATGCTAGTTTCAACAATTCTCGTTCTTTACATTTCTTCCTGG485680.10745419684105283No Hit
GCACAACATCCTTATGCACCCATTCCACATGTTAGGCGTAGCTGGTGTAT462200.1022593678552434No Hit
GAATTTGTATGCTTGGGAGTCCCTGATGATTAAATAAACCAAGATTTTAC459990.10177041674542062No Hit
CTTAGTTTCCGTCTGGGTATGCGTCCTTGGATTGCTGTTGCATATTCAGC455630.10080578921653949No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)