FastQCFastQC Report
Tue 24 Jan 2023
C.Las180.3.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC.Las180.3.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46179200
Sequences flagged as poor quality0
Sequence length20-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGCCTACACAAAGACCAAAATCTTACCCATTTGTAGATGGGGCTTCAA1421440.30780957660591785No Hit
GTTCTATTGAGGGGGACTATCCAAGTACGAGCGAGCCAACTGGGAACCGA1370900.29686525535305935No Hit
CCCTACTCTAATCAAAAGAAAATGCGTTTCGCCTTTTGAAGCGCCAGTCG991170.21463559351396297No Hit
GCACGATTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATC853590.18484295960085928No Hit
CTCATAAGGACCGCCATTGTATAACCATTCATCAACGGATGCCGCTTCCC827960.17929284179890514No Hit
CCGCCATTGTATAACCATTCATCAACGGATGCCGCTTCCCATATCGGGTA792100.17152744092578476No Hit
GGCGATAATAAATACAGAAGTTGCGGTCAATAAAGTAGGGATCATCAAAA767700.1662436768068741No Hit
CGCCATTGTATAACCATTCATCAACGGATGCCGCTTCCCATATCGGGTAA712840.1543638694477167No Hit
GGTCAATAAAGTAGGGATCATCAAAACACCAAACCAACCAATGTAAAGGC686230.148601534890167No Hit
GCGGTCAATAAAGTAGGGATCATCAAAACACCAAACCAACCAATGTAAAG662920.143553807774929No Hit
CAGAAATAATATTGTTTCCATAAAGTAGAGATCCAGAAACAGGTTCACGA660860.14310771949275863No Hit
GGTCAGTTCACCGGGAGGATCGCCCTCCCAATTCGAAGTTTTTTCCTGGA636540.13784127919063127No Hit
CTTGACCGAATCTGTAACCTGCATTAGCAGATTCATTTTCTGTGGTTTCC616250.13344752615896335No Hit
GTTCTATTGAGGGAGACTATCCAAGTACGAGCGAGCCAACTGGGAACCGA615900.13337173446053635No Hit
AGCACGATTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTAT575560.12463619984755042No Hit
CTACGATATTATAAGTTTCTTCCTCTTGACCGAATCTGTAACCTGCATTA572540.12398222576398031No Hit
GTCAATATCTACTGGAGGAGCAGCAATGAAGGCGATAATAAATACAGAAG560260.12132301988774168No Hit
CGCCTACACAAAGACCAAAATCTTACCCATTTGTAGATGGGGCTTCAATA536980.11628178920379739No Hit
GCCGCTTCCCATATCGGGTAAAAATGCAAACCTATAGCTGCAGAAGTAGG526140.11393441202965837No Hit
CGAGAATTGTTGAAACTAGCATATTGGAAGATCAATCGGCCAAAATAACC525710.11384129651444808No Hit
CCCAGACGGAAACTAAGTTCCCACTCACGACCCATGTAACAAGCTACACC508360.11008419374956689No Hit
GTCAGTTCACCGGGAGGATCGCCCTCCCAATTCGAAGTTTTTTCCTGGAA484330.10488055228327905No Hit
CAATAAAGTAGGGATCATCAAAACACCAAACCAACCAATGTAAAGGCGGT480930.10414429006998821No Hit
CAGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGG475030.10286665858221884No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)