FastQCFastQC Report
Tue 24 Jan 2023
C.Las180.3.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC.Las180.3.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46179200
Sequences flagged as poor quality0
Sequence length20-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCTTTTTGCAGAATTTGTATGCTTGGGAGTCCCTGATGATTAAATAA1045270.2263508246136789No Hit
GTTTTGATGATCCCTACTTTATTGACCGCAACTTCTGTATTTATTATCGC722320.1564167417365394No Hit
TGATGATTAAATAAACCAAGATTTTACCATGACTGCAATTTTAGAGAGAC602040.13037038320282723No Hit
GTTTTTCTGATGGTATGCCTCTAGGAATCTCTGGTACTTTCAATTTCATG585760.12684498648742293No Hit
GTTTGCCGACCCGCAGTAGGGACCTCCCGGTCCCCAGAGGCACGTGTCGT575070.12453009146975262No Hit
ATTCGGTCAAGAGGAAGAAACTTATAATATCGTAGCTGCTCACGGTTATT528640.11447578130413692No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)