FastQCFastQC Report
Tue 24 Jan 2023
C.h120.1.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC.h120.1.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46106585
Sequences flagged as poor quality0
Sequence length20-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTGATGATCCCTACTTTATTGACCGCAACTTCTGTATTTATTATCGC1069600.23198421657123378No Hit
GTGGGAACTTAGTTTCCGTCTGGGTATGCGTCCTTGGATTGCTGTTGCAT1056400.22912128495311462No Hit
GCACAACATCCTTATGCACCCATTCCACATGTTAGGCGTAGCTGGTGTAT893630.1938183016590797No Hit
CTCGCTTTTTGCAGAATTTGTATGCTTGGGAGTCCCTGATGATTAAATAA859270.1863660039016119No Hit
GTTAGGCGTAGCTGGTGTATTCGGCGGCTCCCTATTCAGTGCTATGCATG788380.17099075977975814No Hit
GCTTGTTACATGGGTCGTGAGTGGGAACTTAGTTTCCGTCTGGGTATGCG730670.15847410950084462No Hit
GGGAACTTAGTTTCCGTCTGGGTATGCGTCCTTGGATTGCTGTTGCATAT694380.15060321643860633No Hit
GTTTTTCTGATGGTATGCCTCTAGGAATCTCTGGTACTTTCAATTTCATG673590.1460940991400686No Hit
GCAGCGACTGCTGTTTTCTTGATCTACCCAATCGGTCAAGGAAGTTTTTC665920.1444305623589342No Hit
GTTGCATATTCAGCTCCTGTTGCAGCAGCGACTGCTGTTTTCTTGATCTA654470.14194718606897475No Hit
GGGAAACCACAGAAAATGAATCTGCTAATGCAGGTTACAGATTCGGTCAA650120.1410037199675491No Hit
GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTACATGGGTCGTG616720.13375963541867175No Hit
GTTGGTTTGGTGTTTTGATGATCCCTACTTTATTGACCGCAACTTCTGTA602630.13070367280508846No Hit
ATTCGGTCAAGAGGAAGAAACTTATAATATCGTAGCTGCTCACGGTTATT602160.13060173508838271No Hit
TGATGATTAAATAAACCAAGATTTTACCATGACTGCAATTTTAGAGAGAC589460.12784724784973772No Hit
ATTCAGCTCCTGTTGCAGCAGCGACTGCTGTTTTCTTGATCTACCCAATC557390.1208916253502618No Hit
GGATCTCTACTTTATGGAAACAATATTATTTCTGGTGCGATTATTCCTAC528110.11454112248825196No Hit
GTTTGATCAGGGAAACCACAGAAAATGAATCTGCTAATGCAGGTTACAGA527030.11430688262858764No Hit
AGAAAATGAATCTGCTAATGCAGGTTACAGATTCGGTCAAGAGGAAGAAA522110.11323978993456141No Hit
GGAAACCACAGAAAATGAATCTGCTAATGCAGGTTACAGATTCGGTCAAG511670.11097547129113987No Hit
CATGACTGCAATTTTAGAGAGACGCGAAAGCGAACGCCTATGGGGTCGCT508630.11031612946393665No Hit
GGGAAGCGGCATCCGTTGATGAATGGTTATACAATGGCGGTCCTTATGAG501900.10885646811621377No Hit
GGAAGTTTTTCTGATGGTATGCCTCTAGGAATCTCTGGTACTTTCAATTT484480.10507826593533223No Hit
GGAAGCGGCATCCGTTGATGAATGGTTATACAATGGCGGTCCTTATGAGC461640.10012452668095025No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)