FastQCFastQC Report
Tue 24 Jan 2023
C.h120.2.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC.h120.2.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46257600
Sequences flagged as poor quality0
Sequence length20-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGCCTACACAAAGACCAAAATCTTACCCATTTGTAGATGGGGCTTCAA1833620.3963932413268307No Hit
GTTCTATTGAGGGGGACTATCCAAGTACGAGCGAGCCAACTGGGAACCGA1285710.2779456781155962No Hit
GCACGATTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATC1180720.25524886721317147No Hit
GGCGATAATAAATACAGAAGTTGCGGTCAATAAAGTAGGGATCATCAAAA1107960.2395195600290547No Hit
CCCAGACGGAAACTAAGTTCCCACTCACGACCCATGTAACAAGCTACACC1091810.23602824184566426No Hit
CTCATAAGGACCGCCATTGTATAACCATTCATCAACGGATGCCGCTTCCC1044660.22583532219570407No Hit
CCGCCATTGTATAACCATTCATCAACGGATGCCGCTTCCCATATCGGGTA841340.18188146380270484No Hit
AGCACGATTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTAT823800.17808965445678115No Hit
GGTCAATAAAGTAGGGATCATCAAAACACCAAACCAACCAATGTAAAGGC813160.17578949188890042No Hit
GTTCACGAATACCGTCAATATCTACTGGAGGAGCAGCAATGAAGGCGATA735570.15901603196015357No Hit
CCCTACTCTAATCAAAAGAAAATGCGTTTCGCCTTTTGAAGCGCCAGTCG711530.1538190481131749No Hit
CAGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGG701370.1516226522776798No Hit
GCACTGAATAGGGAGCCGCCGAATACACCAGCTACGCCTAACATGTGGAA651780.14090225173809276No Hit
GCCTAACATGTGGAATGGGTGCATAAGGATGTTGTGCTCAGCCTGGAATA646660.13979540659264642No Hit
GCCGCTTCCCATATCGGGTAAAAATGCAAACCTATAGCTGCAGAAGTAGG642060.13880097540728442No Hit
GCCAGGAAGAAATGTAAAGAACGAGAATTGTTGAAACTAGCATATTGGAA637530.13782167687039537No Hit
GTCAATATCTACTGGAGGAGCAGCAATGAAGGCGATAATAAATACAGAAG633190.13688345266507557No Hit
CTACGATATTATAAGTTTCTTCCTCTTGACCGAATCTGTAACCTGCATTA622950.13466976237418285No Hit
GCGGTCAATAAAGTAGGGATCATCAAAACACCAAACCAACCAATGTAAAG612820.1324798519594618No Hit
GGTCAGTTCACCGGGAGGATCGCCCTCCCAATTCGAAGTTTTTTCCTGGA610590.1319977690152537No Hit
GCTAGGTCTAGAGGGAAGTTATGAGCATTACGTTCATGCATAACTTCCAT603810.13053206392030717No Hit
CCAGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGG584090.12626898066479886No Hit
CGAGAATTGTTGAAACTAGCATATTGGAAGATCAATCGGCCAAAATAACC574080.12410501193317422No Hit
CAGAAATAATATTGTTTCCATAAAGTAGAGATCCAGAAACAGGTTCACGA554520.11987651758846114No Hit
CATGTAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG554460.11986354674691294No Hit
CCAGAAACAGGTTCACGAATACCGTCAATATCTACTGGAGGAGCAGCAAT538210.11635061049427553No Hit
AGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGGT533470.11532591401196776No Hit
CCAGACGGAAACTAAGTTCCCACTCACGACCCATGTAACAAGCTACACCA520340.11248746151983674No Hit
AGAAAAACTTCCTTGACCGATTGGGTAGATCAAGAAAACAGCAGTCGCTG495390.10709375324271038No Hit
GCCTACACAAAGACCAAAATCTTACCCATTTGTAGATGGGGCTTCAATAG488980.10570803500397773No Hit
GTTATTTTCAAAAACCCGCCTACACAAAGACCAAAATCTTACCCATTTGT475230.1027355504825153No Hit
GGGTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATGAAATTGAA465020.10052834561239665No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)