FastQCFastQC Report
Tue 24 Jan 2023
C.h180.2.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC.h180.2.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43820619
Sequences flagged as poor quality0
Sequence length20-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGCCTACACAAAGACCAAAATCTTACCCATTTGTAGATGGGGCTTCAA2290450.5226877329140421No Hit
GGCGATAATAAATACAGAAGTTGCGGTCAATAAAG1443460.32940201050103834No Hit
GTTCTATTGAGGGGGACTATCCAAGTACGAGCGAGCCAACTGGGAACCGA1243380.2837431392742307No Hit
GCACGATTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATC1162030.26517881913078406No Hit
CTCATAAGGACCGCCATTGTATAACCATTCATCAACGGATGCCGCTTCCC1089700.2486728907229722No Hit
CCGCCATTGTATAACCATTCATCAACGGATGCCGCTTCCCATATCGGGTA1083210.24719185276684477No Hit
GGCGATAATAAATACAGAAGTTGCGGTCAATAAAGTAGGGATCATCAAAA1058640.24158490321645162No Hit
CAGAAATAATATTGTTTCCATAAAGTAGAGATCCAGAAACAGGTTCACGA953640.21762358035152357No Hit
CCCTACTCTAATCAAAAGAAAATGCGTTTCGCCTTTTGAAGCGCCAGTCG904910.20650324451144791No Hit
CGCCATTGTATAACCATTCATCAACGGATGCCGCTTCCCATATCGGGTAA891500.20344304127698423No Hit
GGTCAATAAAGTAGGGATCATCAAAACACCAAACCAACCAATGTAAAGGC856580.1954741899013339No Hit
CCCAGACGGAAACTAAGTTCCCACTCACGACCCATGTAACAAGCTACACC818470.18677737071673953No Hit
AGCACGATTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTAT815260.18604483884629744No Hit
CGCCTACACAAAGACCAAAATCTTACCCATTTGTAGATGGGGCTTCAATA798610.18224525764914457No Hit
GTCAATATCTACTGGAGGAGCAGCAATGAAGGCGATAATAAATACAGAAG733520.16739151950363823No Hit
GCGGTCAATAAAGTAGGGATCATCAAAACACCAAACCAACCAATGTAAAG717860.1638178593506404No Hit
CTTGACCGAATCTGTAACCTGCATTAGCAGATTCATTTTCTGTGGTTTCC716410.1634869648920295No Hit
CTACGATATTATAAGTTTCTTCCTCTTGACCGAATCTGTAACCTGCATTA708170.1616065715548199No Hit
CATGTAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG671720.1532885694745663No Hit
GGTCAGTTCACCGGGAGGATCGCCCTCCCAATTCGAAGTTTTTTCCTGGA663170.15133743318413645No Hit
GCCGCTTCCCATATCGGGTAAAAATGCAAACCTATAGCTGCAGAAGTAGG661900.15104761527900826No Hit
CGAGAATTGTTGAAACTAGCATATTGGAAGATCAATCGGCCAAAATAACC631360.14407829337143777No Hit
CACGATTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATCA594370.13563706163073597No Hit
CAATAAAGTAGGGATCATCAAAACACCAAACCAACCAATGTAAAGGCGGT590500.1347539157308572No Hit
CAGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGG589860.1346078657629186No Hit
GTTCACGAATACCGTCAATATCTACTGGAGGAGCAGCAATGAAGGCGATA584220.1333208004204596No Hit
GTTCTATTGAGGGAGACTATCCAAGTACGAGCGAGCCAACTGGGAACCGA569070.12986352383566285No Hit
CAGATACCTACTACAGGCCAAGCAGCCAGGAAGAAATGTAAAGAACGAGA561070.12803789923643022No Hit
CCAGAAACAGGTTCACGAATACCGTCAATATCTACTGGAGGAGCAGCAAT555020.12665727063326054No Hit
GCTAGGTCTAGAGGGAAGTTATGAGCATTACGTTCATGCATAACTTCCAT547600.12496400381747232No Hit
GCCTACACAAAGACCAAAATCTTACCCATTTGTAGATGGGGCTTCAATAG542190.12372942518224127No Hit
CTACTACAGGCCAAGCAGCCAGGAAGAAATGTAAAGAACGAGAATTGTTG537630.12268881916067868No Hit
CAGACGGAAACTAAGTTCCCACTCACGACCCATGTAACAAGCTACACCAA521920.11910374885393564No Hit
AGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGGT506850.11566472851513121No Hit
GCACTGAATAGGGAGCCGCCGAATACACCAGCTACGCCTAACATGTGGAA495220.11301072675399679No Hit
GTCAGTTCACCGGGAGGATCGCCCTCCCAATTCGAAGTTTTTTCCTGGAA494260.11279165180208889No Hit
AGAAAAACTTCCTTGACCGATTGGGTAGATCAAGAAAACAGCAGTCGCTG494050.11274372915635901No Hit
GTCAATAAAGTAGGGATCATCAAAACACCAAACCAACCAATGTAAAGGCG491150.1120819402391372No Hit
CCAGACGGAAACTAAGTTCCCACTCACGACCCATGTAACAAGCTACACCA485720.11084279754240807No Hit
GCGATAATAAATACAGAAGTTGCGGTCAATAAAGTAGGGATCATCAAAAC478940.10929558069455841No Hit
ACCAGAAATAATATTGTTTCCATAAAGTAGAGATCCAGAAACAGGTTCAC476970.10884602063699739No Hit
GTTATTTTCAAAAACCCGCCTACACAAAGACCAAAATCTTACCCATTTGT461470.1053088729759842No Hit
GTTCTATTGAGAGGGACCATCCAGGTATGAGCGGACTGACTGGGAACCGA455720.10399670529528578No Hit
GCCTAACATGTGGAATGGGTGCATAAGGATGTTGTGCTCAGCCTGGAATA454640.10375024597438937No Hit
CTGGAAGTTTCCACCTTGTTGACTATGACAACAGTCGCGACTATAAACAG452450.10325048124034944No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)