FastQCFastQC Report
Tue 24 Jan 2023
C.h180.2.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC.h180.2.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43820619
Sequences flagged as poor quality0
Sequence length20-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTATTGACCGCAACTTCTGTATTTATTATCGCC1425480.325298919214263No Hit
CTCGCTTTTTGCAGAATTTGTATGCTTGGGAGTCCCTGATGATTAAATAA1318400.30086293395353453No Hit
GTTTTGATGATCCCTACTTTATTGACCGCAACTTCTGTATTTATTATCGC921140.21020698041714106No Hit
CTTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATTGCTGCTCCT817510.18655829576483163No Hit
TGATGATTAAATAAACCAAGATTTTACCATGACTGCAATTTTAGAGAGAC751390.17146950845217407No Hit
GTTTTTCTGATGGTATGCCTCTAGGAATCTCTGGTACTTTCAATTTCATG715400.16325647978637636No Hit
ATCTGGTTCACTGCTTTAGGTATTAGCACTATGGCTTTCAACTTAAATGG619900.14146308613303707No Hit
ATTCGGTCAAGAGGAAGAAACTTATAATATCGTAGCTGCTCACGGTTATT612950.13987707476245373No Hit
GTTTGCCGACCCGCAGTAGGGACCTCCCGGTCCCCAGAGGCACGTGTCGT591260.1349273500677843No Hit
TCTCGCTTTTTGCAGAATTTGTATGCTTGGGAGTCCCTGATGATTAAATA590210.134687736839135No Hit
CTTCCAATATGCTAGTTTCAACAATTCTCGTTCTTTACATTTCTTCCTGG565120.12896212168979174No Hit
GTGGGAACTTAGTTTCCGTCTGGGTATGCGTCCTTGGATTGCTGTTGCAT556320.12695393463063587No Hit
GAATTTGTATGCTTGGGAGTCCCTGATGATTAAATAAACCAAGATTTTAC547770.12500279834020603No Hit
CTTAGTTTCCGTCTGGGTATGCGTCCTTGGATTGCTGTTGCATATTCAGC526110.12005991973778371No Hit
ATTCCTACTTCTGCAGCTATAGGTTTGCATTTTTACCCGATATGGGAAGC509360.11623751823314044No Hit
GCACAACATCCTTATGCACCCATTCCACATGTTAGGCGTAGCTGGTGTAT497800.11359949068724931No Hit
CGTGAACCTGTTTCTGGATCTCTACTTTATGGAAACAATATTATTTCTGG485570.11080856708117245No Hit
CTTCTGTAACTGGATAACTAGCACTGAAAACCGCCTTTACATTGGTTGGT483240.11027685391664596No Hit
GTTAGGCGTAGCTGGTGTATTCGGCGGCTCCCTATTCAGTGCTATGCATG471570.10761372403251539No Hit
CTGATGATTAAATAAACCAAGATTTTACCATGACTGCAATTTTAGAGAGA461070.10521759174602256No Hit
CAACATCCTTATGCACCCATTCCACATGTTAGGCGTAGCTGGTGTATTCG458170.10455580282880075No Hit
CTAGCACTGAAAACCGCCTTTACATTGGTTGGTTTGGTGTTTTGATGATC448770.10241069392470242No Hit
CGGTATTCGTGAACCTGTTTCTGGATCTCTACTTTATGGAAACAATATTA446470.10188582685242305No Hit
GTTATTTTGGCCGATTGATCTTCCAATATGCTAGTTTCAACAATTCTCGT445500.1016644698697661No Hit
ATTATTTCTGGTGCGATTATTCCTACTTCTGCAGCTATAGGTTTGCATTT438910.10016061160614824No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)