FastQCFastQC Report
Tue 24 Jan 2023
M.Las10.5.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM.Las10.5.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46000870
Sequences flagged as poor quality0
Sequence length20-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTACTCTAAAGAAAATTCGTTTCGCCTTTTGAAGCGCCAGTCGCGTAG781290.16984243993646206No Hit
CCGGATTTTCAAGGGCCGCCGGGGGCGCACCGGACACCGCGCGACGTGCG773990.16825551342833298No Hit
GGTCAGTTCACCGGGAGGATCGCCCTCCCAATTCGAAGTTTTTTCCTGGA695730.1512427917124176No Hit
CTGCAAAGGATTCTACCCGCCGCTCGGTGGGAATTAAGATTCAAGGCGGC619800.1347365821559462No Hit
CCCGACTCGCAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCGCGACGG561970.12216508079086331No Hit
GTCAGTTCACCGGGAGGATCGCCCTCCCAATTCGAAGTTTTTTCCTGGAA521350.11333481301549297No Hit
CCGCCATTGTATAACCATTCATCAACGGATGCCGCTTCCCATATCGGGTA468440.1018328566394505No Hit
CTGGAAGTTTCCACCTTGTTGACTATGACAACAGTCGCGACTATAAACAG462210.10047853442771842No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)