FastQCFastQC Report
Tue 24 Jan 2023
M.h10.5.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM.h10.5.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45954496
Sequences flagged as poor quality0
Sequence length20-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAAGTTTACCTACTACTGTACCGGCGTGAATATGATCTCCACCAGACA767650.16704567927368846No Hit
CTACGATATTATAAGTTTCTTCCTCTTGACCGAATCTGTAACCTGCATTA737500.16048484135262847No Hit
CTTGACCGAATCTGTAACCTGCATTAGCAGATTCATTTTCTGTGGTTTCC661920.14403813720424657No Hit
CTCAGCCTGGAATACAATCATGAAGTTGAAAGTACCAGAGATTCCTAGAG636030.13840430324815226No Hit
CTCATAAGGACCGCCATTGTATAACCATTCATCAACGGATGCCGCTTCCC554930.12075641086347678No Hit
CCCAGGTATTAATTACACGACCTTGACTATCAACTACGGATTGGTTGAAA481220.10471663099079576No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)