FastQCFastQC Report
Sat 1 Apr 2023
C.Las.030.3.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC.Las.030.3.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47141714
Total Bases6.2 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACTAAGCCGTATCTCGTATGCCGT3975610.8433316616362315TruSeq Adapter, Index 3 (96% over 32bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2423220.5140288280566124No Hit
CGAGAATTGTTGAAACTAGCATATTGGAAGATCAATCGGCCAAAATAACC819700.17387997390167018No Hit
CTACGATATTATAAGTTTCTTCCTCTTGACCGAATCTGTAACCTGCATTA819590.17385664000252515No Hit
GGTCAATAAAGTAGGGATCATCAAAACACCAAACCAACCAATGTAAAGGC744510.15793019320426066No Hit
GCGGTCAATAAAGTAGGGATCATCAAAACACCAAACCAACCAATGTAAAG743610.1577392794839831No Hit
CAGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGG721820.1531170461897079No Hit
GTTCTATTGAGGGGGACTATCCAAGTACGAGCGAGCCAACTGGGAACCGA678750.1439807640426481No Hit
AGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGGT663810.14081159628604084No Hit
TCTTGACCGAATCTGTAACCTGCATTAGCAGATTCATTTTCTGTGGTTTC657180.13940519854666295No Hit
GGCGATAATAAATACAGAAGTTGCGGTCAATAAAGTAGGGATCATCAAAA627780.13316868368426316No Hit
GCACTGAATAGGGAGCCGCCGAATACACCAGCTACGCCTAACATGTGGAA603550.12802886208167993No Hit
GCACGATTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATC590220.12520121775801363No Hit
GCCTAACATGTGGAATGGGTGCATAAGGATGTTGTGCTCAGCCTGGAATA574240.1218114385913079No Hit
GGTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATGAAATTGAAA522140.11075965545079673No Hit
GTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATGAAATTGAAAG520410.11039267685515211No Hit
GCCAGGAAGAAATGTAAAGAACGAGAATTGTTGAAACTAGCATATTGGAA519490.11019752060775728No Hit
CTTGACCGAATCTGTAACCTGCATTAGCAGATTCATTTTCTGTGGTTTCC502930.10668470815465046No Hit
GTGGAATGGGTGCATAAGGATGTTGTGCTCAGCCTGGAATACAATCATGA497670.10556892352280615No Hit
GCCGCTTCCCATATCGGGTAAAAATGCAAACCTATAGCTGCAGAAGTAGG488940.10371706043611398No Hit
GCAGCTACGATATTATAAGTTTCTTCCTCTTGACCGAATCTGTAACCTGC487240.10335644563114527No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)