FastQCFastQC Report
Sat 1 Apr 2023
C.Las.180.2.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC.Las.180.2.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45198793
Total Bases6.5 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGCCTACACAAAGACCAAAATCTTACCCATTTGTAGATGGGGCTTCAA2062610.45634183195998174No Hit
GTTCTATTGAGGGGGACTATCCAAGTACGAGCGAGCCAACTGGGAACCGA1503520.3326460509686619No Hit
GCACGATTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATC1045430.23129599943078125No Hit
CCGCCATTGTATAACCATTCATCAACGGATGCCGCTTCCCATATCGGGTA1039420.2299663179058786No Hit
CCCTACTCTAATCAAAAGAAAATGCGTTTCGCCTTTTGAAGCGCCAGTCG953340.21092156155585834No Hit
GGCGATAATAAATACAGAAGTTGCGGTCAATAAAGTAGGGATCATCAAAA929540.20565593421930536No Hit
CTCATAAGGACCGCCATTGTATAACCATTCATCAACGGATGCCGCTTCCC913630.2021359287182735No Hit
CGCCATTGTATAACCATTCATCAACGGATGCCGCTTCCCATATCGGGTAA824730.18246726190232557No Hit
AGCACGATTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTAT788660.17448696030444882No Hit
CAGAAATAATATTGTTTCCATAAAGTAGAGATCCAGAAACAGGTTCACGA775510.17157759057858027No Hit
GGTCAATAAAGTAGGGATCATCAAAACACCAAACCAACCAATGTAAAGGC764630.16917044665329892No Hit
GCGGTCAATAAAGTAGGGATCATCAAAACACCAAACCAACCAATGTAAAG725710.16055959724411226No Hit
CGCCTACACAAAGACCAAAATCTTACCCATTTGTAGATGGGGCTTCAATA715760.15835821102567937No Hit
CCCAGACGGAAACTAAGTTCCCACTCACGACCCATGTAACAAGCTACACC708060.15665462571091224No Hit
CTTGACCGAATCTGTAACCTGCATTAGCAGATTCATTTTCTGTGGTTTCC658280.14564105727336568No Hit
CTACGATATTATAAGTTTCTTCCTCTTGACCGAATCTGTAACCTGCATTA657390.14544414936036013No Hit
GGTCAGTTCACCGGGAGGATCGCCCTCCCAATTCGAAGTTTTTTCCTGGA647620.14328258721422052No Hit
GTCAATATCTACTGGAGGAGCAGCAATGAAGGCGATAATAAATACAGAAG647270.14320515151809474No Hit
GTTCTATTGAGGGAGACTATCCAAGTACGAGCGAGCCAACTGGGAACCGA647200.1431896643788696No Hit
GGCGATAATAAATACAGAAGTTGCGGTCAATAAAG584140.1292379643854649No Hit
GCCGCTTCCCATATCGGGTAAAAATGCAAACCTATAGCTGCAGAAGTAGG582820.12894592118864767No Hit
CATGTAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG573780.12694586778014183No Hit
CGAGAATTGTTGAAACTAGCATATTGGAAGATCAATCGGCCAAAATAACC557580.12336170127374863No Hit
CACGATTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATCA522900.11568893001191426No Hit
CAGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGG513270.11355834214422494No Hit
CAATAAAGTAGGGATCATCAAAACACCAAACCAACCAATGTAAAGGCGGT500820.11080384381060795No Hit
CTACTACAGGCCAAGCAGCCAGGAAGAAATGTAAAGAACGAGAATTGTTG491910.10883255223209168No Hit
GCTAGGTCTAGAGGGAAGTTATGAGCATTACGTTCATGCATAACTTCCAT477570.10565990113939547No Hit
GTTATTTTCAAAAACCCGCCTACACAAAGACCAAAATCTTACCCATTTGT461240.10204697280301268No Hit
GTTCACGAATACCGTCAATATCTACTGGAGGAGCAGCAATGAAGGCGATA459050.10156244659011138No Hit
GTTCTATTGAGAGGGACCATCCAGGTATGAGCGGACTGACTGGGAACCGA457640.10125049135714752No Hit
CTGGAAGTTTCCACCTTGTTGACTATGACAACAGTCGCGACTATAAACAG457170.1011465062794929No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)