FastQCFastQC Report
Sat 1 Apr 2023
C.h.030.1.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC.h.030.1.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26571312
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1689740.6359264457848375No Hit
CCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGG668720.251669921304601No Hit
CTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT458170.17243032636100167No Hit
ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA400720.1508092637653722No Hit
GTTTGCCGACCCGCAGTAGGGACCTCCCGGTCCCCAGAGGCACGTGTCGT385600.1451189162206217No Hit
GCAGCGACTGCTGTTTTCTTGATCTACCCAATCGGTCAAGGAAGTTTTTC379200.14271030350326697No Hit
GTTAGGCGTAGCTGGTGTATTCGGCGGCTCCCTATTCAGTGCTATGCATG365290.13747533430039133No Hit
GCACAACATCCTTATGCACCCATTCCACATGTTAGGCGTAGCTGGTGTAT337930.12717851493369992No Hit
GCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACCACAGAAAATG336010.12645593111849351No Hit
GCCCAATCCAAGTAATGAGTGATCCACGTGGTCCGGGCGAAGGTCTGGAT333180.1253908726825382No Hit
CGGCGGCTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTT301400.1134306051579237No Hit
CCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGG300060.11292630187022755No Hit
GTTCCACCCGAGGAAGCGGGGGCTGCGGTAGCTGCGGAATCTTCTACTGG299710.11279458086224721No Hit
GTTTGATCAGGGAAACCACAGAAAATGAATCTGCTAATGCAGGTTACAGA280700.10564024840022954No Hit
GTTTTTCTGATGGTATGCCTCTAGGAATCTCTGGTACTTTCAATTTCATG280180.10544454861694448No Hit
CTCGCTTTTTGCAGAATTTGTATGCTTGGGAGTCCCTGATGATTAAATAA265910.10007409494871763No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)