FastQCFastQC Report
Sat 1 Apr 2023
C.h.120.1.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC.h.120.1.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46106585
Total Bases6.6 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTGATGATCCCTACTTTATTGACCGCAACTTCTGTATTTATTATCGC1070550.23219026089223482No Hit
GTGGGAACTTAGTTTCCGTCTGGGTATGCGTCCTTGGATTGCTGTTGCAT1056830.22921454711946243No Hit
GCACAACATCCTTATGCACCCATTCCACATGTTAGGCGTAGCTGGTGTAT896030.19433883468055593No Hit
CTCGCTTTTTGCAGAATTTGTATGCTTGGGAGTCCCTGATGATTAAATAA859880.1864983060445704No Hit
GTTAGGCGTAGCTGGTGTATTCGGCGGCTCCCTATTCAGTGCTATGCATG788650.17104931974467422No Hit
GCTTGTTACATGGGTCGTGAGTGGGAACTTAGTTTCCGTCTGGGTATGCG730840.15851098058986585No Hit
GGGAACTTAGTTTCCGTCTGGGTATGCGTCCTTGGATTGCTGTTGCATAT694690.15067045195388035No Hit
GTTTTTCTGATGGTATGCCTCTAGGAATCTCTGGTACTTTCAATTTCATG674250.14623724572097457No Hit
GCAGCGACTGCTGTTTTCTTGATCTACCCAATCGGTCAAGGAAGTTTTTC667440.1447602332725358No Hit
GTTGCATATTCAGCTCCTGTTGCAGCAGCGACTGCTGTTTTCTTGATCTA655470.14216407482792315No Hit
GGGAAACCACAGAAAATGAATCTGCTAATGCAGGTTACAGATTCGGTCAA652030.14141797749714058No Hit
GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTACATGGGTCGTG618640.13417606183585273No Hit
GTTGGTTTGGTGTTTTGATGATCCCTACTTTATTGACCGCAACTTCTGTA603320.13085332604876287No Hit
ATTCGGTCAAGAGGAAGAAACTTATAATATCGTAGCTGCTCACGGTTATT603030.13079042830866783No Hit
TGATGATTAAATAAACCAAGATTTTACCATGACTGCAATTTTAGAGAGAC589920.127947016678854No Hit
ATTCAGCTCCTGTTGCAGCAGCGACTGCTGTTTTCTTGATCTACCCAATC557920.12100657639250445No Hit
GGATCTCTACTTTATGGAAACAATATTATTTCTGGTGCGATTATTCCTAC528350.11459317579039956No Hit
GTTTGATCAGGGAAACCACAGAAAATGAATCTGCTAATGCAGGTTACAGA527430.114393638132167No Hit
AGAAAATGAATCTGCTAATGCAGGTTACAGATTCGGTCAAGAGGAAGAAA522770.11338293651546737No Hit
GGAAACCACAGAAAATGAATCTGCTAATGCAGGTTACAGATTCGGTCAAG512490.11115332007347756No Hit
CATGACTGCAATTTTAGAGAGACGCGAAAGCGAACGCCTATGGGGTCGCT509430.1104896404710954No Hit
GGGAAGCGGCATCCGTTGATGAATGGTTATACAATGGCGGTCCTTATGAG502430.10897141915845643No Hit
GGAAGTTTTTCTGATGGTATGCCTCTAGGAATCTCTGGTACTTTCAATTT485320.1052604524928489No Hit
GGAAGCGGCATCCGTTGATGAATGGTTATACAATGGCGGTCCTTATGAGC461860.10017224220791889No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)