FastQCFastQC Report
Sat 1 Apr 2023
C.h.180.2.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC.h.180.2.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43820619
Total Bases6.3 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGCCTACACAAAGACCAAAATCTTACCCATTTGTAGATGGGGCTTCAA2291710.5229752687884213No Hit
GGCGATAATAAATACAGAAGTTGCGGTCAATAAAG1443460.32940201050103834No Hit
GTTCTATTGAGGGGGACTATCCAAGTACGAGCGAGCCAACTGGGAACCGA1243590.28379106191996056No Hit
GGCGATAATAAATACAGAAGTTGCGGTCAATAAAGTAGGGATCATCAAAA1184290.2702586195781488No Hit
GCACGATTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATC1162660.2653225870679737No Hit
CTCATAAGGACCGCCATTGTATAACCATTCATCAACGGATGCCGCTTCCC1094280.2497180608060329No Hit
CCGCCATTGTATAACCATTCATCAACGGATGCCGCTTCCCATATCGGGTA1089100.24853596887802978No Hit
CAGAAATAATATTGTTTCCATAAAGTAGAGATCCAGAAACAGGTTCACGA972160.22184990129874704No Hit
GTCAATATCTACTGGAGGAGCAGCAATGAAGGCGATAATAAATACAGAAG913910.2085570721855846No Hit
CCCTACTCTAATCAAAAGAAAATGCGTTTCGCCTTTTGAAGCGCCAGTCG905860.20672003743260678No Hit
CGCCATTGTATAACCATTCATCAACGGATGCCGCTTCCCATATCGGGTAA896710.20463197929723448No Hit
GGTCAATAAAGTAGGGATCATCAAAACACCAAACCAACCAATGTAAAGGC864210.197215379362852No Hit
CCCAGACGGAAACTAAGTTCCCACTCACGACCCATGTAACAAGCTACACC822920.18779287440006268No Hit
AGCACGATTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTAT815570.18611558179951773No Hit
CGCCTACACAAAGACCAAAATCTTACCCATTTGTAGATGGGGCTTCAATA798780.18228405217187826No Hit
GCGGTCAATAAAGTAGGGATCATCAAAACACCAAACCAACCAATGTAAAG792690.18089429544571245No Hit
CTTGACCGAATCTGTAACCTGCATTAGCAGATTCATTTTCTGTGGTTTCC717130.16365127110596042No Hit
CTACGATATTATAAGTTTCTTCCTCTTGACCGAATCTGTAACCTGCATTA710630.16216795111908391No Hit
GCCGCTTCCCATATCGGGTAAAAATGCAAACCTATAGCTGCAGAAGTAGG675490.15414889506695467No Hit
CATGTAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG675250.15409412632897768No Hit
GGTCAGTTCACCGGGAGGATCGCCCTCCCAATTCGAAGTTTTTTCCTGGA663400.15138991989136438No Hit
CGAGAATTGTTGAAACTAGCATATTGGAAGATCAATCGGCCAAAATAACC640120.14607735230759747No Hit
CAATAAAGTAGGGATCATCAAAACACCAAACCAACCAATGTAAAGGCGGT611240.13948684750436774No Hit
GTTCACGAATACCGTCAATATCTACTGGAGGAGCAGCAATGAAGGCGATA596600.13614595448777206No Hit
CACGATTAATAATATCAGCCCAGGTATTAATTACACGACCTTGACTATCA594600.13568954833796393No Hit
CAGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGG592750.13526737264939137No Hit
CAGATACCTACTACAGGCCAAGCAGCCAGGAAGAAATGTAAAGAACGAGA570440.13017616204828142No Hit
GTTCTATTGAGGGAGACTATCCAAGTACGAGCGAGCCAACTGGGAACCGA569110.129872651958659No Hit
CCAGAAACAGGTTCACGAATACCGTCAATATCTACTGGAGGAGCAGCAAT559050.127576929025124No Hit
GCTAGGTCTAGAGGGAAGTTATGAGCATTACGTTCATGCATAACTTCCAT548130.1250849514471715No Hit
CTACTACAGGCCAAGCAGCCAGGAAGAAATGTAAAGAACGAGAATTGTTG547780.12500508037095504No Hit
GCCTACACAAAGACCAAAATCTTACCCATTTGTAGATGGGGCTTCAATAG542400.12377734782797112No Hit
GCGATAATAAATACAGAAGTTGCGGTCAATAAAGTAGGGATCATCAAAAC525590.11994125413883361No Hit
GTCAATAAAGTAGGGATCATCAAAACACCAAACCAACCAATGTAAAGGCG524200.11962405186471693No Hit
CAGACGGAAACTAAGTTCCCACTCACGACCCATGTAACAAGCTACACCAA523910.11955787297299474No Hit
GCACTGAATAGGGAGCCGCCGAATACACCAGCTACGCCTAACATGTGGAA510150.11641779866231464No Hit
ACCAGAAATAATATTGTTTCCATAAAGTAGAGATCCAGAAACAGGTTCAC509180.1161964416796577No Hit
AGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGGT509100.11617818543366537No Hit
AGAAAAACTTCCTTGACCGATTGGGTAGATCAAGAAAACAGCAGTCGCTG495120.11298790644650639No Hit
GTCAGTTCACCGGGAGGATCGCCCTCCCAATTCGAAGTTTTTTCCTGGAA494370.11281675414032834No Hit
CCAGACGGAAACTAAGTTCCCACTCACGACCCATGTAACAAGCTACACCA488100.11138592086067976No Hit
GTTATTTTCAAAAACCCGCCTACACAAAGACCAAAATCTTACCCATTTGT461630.10534538546796886No Hit
GCCAGGAAGAAATGTAAAGAACGAGAATTGTTGAAACTAGCATATTGGAA460590.10510805427006863No Hit
GTTCTATTGAGAGGGACCATCCAGGTATGAGCGGACTGACTGGGAACCGA455820.10401952560277618No Hit
GCCTAACATGTGGAATGGGTGCATAAGGATGTTGTGCTCAGCCTGGAATA455770.10400811544903096No Hit
CTGGAAGTTTCCACCTTGTTGACTATGACAACAGTCGCGACTATAAACAG452510.10326417342484367No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)