FastQCFastQC Report
Sat 1 Apr 2023
C.h.180.2.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC.h.180.2.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43820619
Total Bases6.3 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTATTGACCGCAACTTCTGTATTTATTATCGCC1425480.325298919214263No Hit
CTCGCTTTTTGCAGAATTTGTATGCTTGGGAGTCCCTGATGATTAAATAA1318730.30093824096825283No Hit
CTTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATTGCTGCTCCT1110220.2533556178200039No Hit
GTTTTGATGATCCCTACTTTATTGACCGCAACTTCTGTATTTATTATCGC978370.22326704239390135No Hit
TGATGATTAAATAAACCAAGATTTTACCATGACTGCAATTTTAGAGAGAC752130.1716383787276031No Hit
GTTTTTCTGATGGTATGCCTCTAGGAATCTCTGGTACTTTCAATTTCATG723150.16502505361688297No Hit
ATCTGGTTCACTGCTTTAGGTATTAGCACTATGGCTTTCAACTTAAATGG619950.14147449628678224No Hit
ATTCGGTCAAGAGGAAGAAACTTATAATATCGTAGCTGCTCACGGTTATT613580.14002084269964327No Hit
GTTTGCCGACCCGCAGTAGGGACCTCCCGGTCCCCAGAGGCACGTGTCGT591340.1349456063137766No Hit
TCTCGCTTTTTGCAGAATTTGTATGCTTGGGAGTCCCTGATGATTAAATA590480.13474935166935914No Hit
CTTCCAATATGCTAGTTTCAACAATTCTCGTTCTTTACATTTCTTCCTGG586250.13378405266251486No Hit
GTGGGAACTTAGTTTCCGTCTGGGTATGCGTCCTTGGATTGCTGTTGCAT556960.12709998459857447No Hit
GAATTTGTATGCTTGGGAGTCCCTGATGATTAAATAAACCAAGATTTTAC548020.12505984910893203No Hit
CGTGAACCTGTTTCTGGATCTCTACTTTATGGAAACAATATTATTTCTGG534470.1219676974439818No Hit
CTTAGTTTCCGTCTGGGTATGCGTCCTTGGATTGCTGTTGCATATTCAGC528690.12064868367103623No Hit
ATTCCTACTTCTGCAGCTATAGGTTTGCATTTTTACCCGATATGGGAAGC520900.11887098171753348No Hit
GCACAACATCCTTATGCACCCATTCCACATGTTAGGCGTAGCTGGTGTAT511730.1167783595206631No Hit
CTTCTGTAACTGGATAACTAGCACTGAAAACCGCCTTTACATTGGTTGGT483760.11039551951559606No Hit
CTAGCACTGAAAACCGCCTTTACATTGGTTGGTTTGGTGTTTTGATGATC472000.10771185135472414No Hit
GTTAGGCGTAGCTGGTGTATTCGGCGGCTCCCTATTCAGTGCTATGCATG471980.10770728729322604No Hit
CAACATCCTTATGCACCCATTCCACATGTTAGGCGTAGCTGGTGTATTCG471960.10770272323172797No Hit
GTTATTTTGGCCGATTGATCTTCCAATATGCTAGTTTCAACAATTCTCGT466640.10648868287323827No Hit
CTGATGATTAAATAAACCAAGATTTTACCATGACTGCAATTTTAGAGAGA461910.10540928232894199No Hit
CGGTATTCGTGAACCTGTTTCTGGATCTCTACTTTATGGAAACAATATTA461360.10528377063774474No Hit
ATTATTTCTGGTGCGATTATTCCTACTTCTGCAGCTATAGGTTTGCATTT446370.10186300654493265No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)