FastQCFastQC Report
Mon 12 Jun 2023
M.Las.010.4.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM.Las.010.4.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43599644
Total Bases6.2 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGATTTTCAAGGGCCGCCGGGGGCGCACCGGACACCGCGCGACGTGCG692490.15882927851429246No Hit
GTCCTATTGAGAGAGACCATCCAAGTATGCACGGGCCAACTGGGAACCGA641490.14713193529745336No Hit
CTGCAAAGGATTCTACCCGCCGCTCGGTGGGAATTAAGATTCAAGGCGGC568630.1304207896743377No Hit
CCCTACTCTAAAGAAAATTCGTTTCGCCTTTTGAAGCGCCAGTCGCGTAG506410.11615003094979398No Hit
CCCGACTCGCAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCGCGACGG486480.11157889270839No Hit
CAGAGATTCCTAGAGGCATACCATCAGAAAAACTTCCTTGACCGATTGGG474850.10891143973560885No Hit
GGTCAGTTCACCGGGAGGATCGCCCTCCCAATTCGAAGTTTTTTCCTGGA472320.10833115976818526No Hit
GTTCATATTAGGGAAAGGAGAGTGAGGGGAAGAGTCAACTACGTCGAACC442170.10141596568999509No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)